H1KE76 · H1KE76_METEX
- ProteinNADPH-dependent 7-cyano-7-deazaguanine reductase
- GenequeF
- StatusUniProtKB unreviewed (TrEMBL)
- Amino acids153 (go to sequence)
- Protein existenceInferred from homology
- Annotation score3/5
Function
function
Catalyzes the NADPH-dependent reduction of 7-cyano-7-deazaguanine (preQ0) to 7-aminomethyl-7-deazaguanine (preQ1).
Catalytic activity
- 7-aminomethyl-7-carbaguanine + 2 NADP+ = 7-cyano-7-deazaguanine + 2 NADPH + 3 H+
Pathway
tRNA modification; tRNA-queuosine biosynthesis.
Features
Showing features for active site, binding site.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Active site | 51 | Thioimide intermediate | ||||
Sequence: C | ||||||
Active site | 58 | Proton donor | ||||
Sequence: D | ||||||
Binding site | 73-75 | substrate | ||||
Sequence: VES | ||||||
Binding site | 92-93 | substrate | ||||
Sequence: HE |
GO annotations
Aspect | Term | |
---|---|---|
Cellular Component | cytoplasm | |
Molecular Function | preQ1 synthase activity | |
Biological Process | queuosine biosynthetic process | |
Biological Process | tRNA modification |
Keywords
- Molecular function
- Biological process
- Ligand
Enzyme and pathway databases
Names & Taxonomy
Protein names
- Recommended nameNADPH-dependent 7-cyano-7-deazaguanine reductase
- EC number
- Alternative names
Gene names
Organism names
- Strain
- Taxonomic lineageBacteria > Pseudomonadota > Alphaproteobacteria > Hyphomicrobiales > Methylobacteriaceae > Methylorubrum
Accessions
- Primary accessionH1KE76
Proteomes
Subcellular Location
Structure
Family & Domains
Features
Showing features for compositional bias, region.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Compositional bias | 1-20 | Polar residues | ||||
Sequence: MDSIETHAKQLGQQTPLPAS | ||||||
Region | 1-30 | Disordered | ||||
Sequence: MDSIETHAKQLGQQTPLPASPEAAQLDRVP |
Sequence similarities
Belongs to the GTP cyclohydrolase I family. QueF type 1 subfamily.
Family and domain databases
Sequence
- Sequence statusComplete
- Length153
- Mass (Da)17,119
- Last updated2012-03-21 v1
- Checksum81380DD3012D5344
Features
Showing features for compositional bias.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Compositional bias | 1-20 | Polar residues | ||||
Sequence: MDSIETHAKQLGQQTPLPAS |
Sequence databases
Nucleotide Sequence | Protein Sequence | Molecule Type | Status | |
---|---|---|---|---|
AGJK01000014 EMBL· GenBank· DDBJ | EHP94193.1 EMBL· GenBank· DDBJ | Genomic DNA |