H0PPF0 · H0PPF0_9ADEN

  • Protein
    Packaging protein 1
  • Gene
    IVa2
  • Status
    UniProtKB unreviewed (TrEMBL)
  • Amino acids
  • Protein existence
    Evidence at transcript level
  • Annotation score
    3/5

Function

function

Component of the packaging machinery which encapsidates the viral DNA into preformed capsids and transcriptional activator of the viral major late promoter (MLP). Binds, along with packaging proteins 2 and 3, to the specific packaging sequence on the left end of viral genomic DNA and displays ATPase activity thereby providing the power stroke of the packaging machinery. The activity of packaging protein IVa2 is stimulated by protein 33K which acts as a terminase. May be the protein that pumps DNA into the capsid powered by ATP hydrolysis. Specifically binds to the 5'-CG-3' nucleotides of the repeats making up the packaging sequence. Component of the DEF-A and DEF-B transcription factors that bind downstream elements of the major late promoter (MLP), and stimulate transcription from the MLP after initiation of viral DNA replication. DEF-A is a heterodimer packaging proteins 1 and 2 and DEF-B is a homodimer of packaging protein 1.

Features

Showing features for binding site.

144850100150200250300350400
TypeIDPosition(s)Description
Binding site171-178ATP (UniProtKB | ChEBI)

GO annotations

AspectTerm
Cellular Componenthost cell nucleolus
Cellular Componenthost cell nucleoplasm
Cellular Componentvirion component
Molecular FunctionATP binding
Molecular FunctionATP hydrolysis activity
Molecular FunctionDNA binding
Biological ProcessDNA-templated transcription
Biological Processnuclear capsid assembly
Biological Processregulation of DNA-templated transcription
Biological Processviral DNA genome packaging via site-specific sequence recognition
Biological Processviral release from host cell
Biological Processviral transcription

Keywords

Names & Taxonomy

Protein names

  • Recommended name
    Packaging protein 1
  • Alternative names
    • Packaging protein IVa2

Gene names

    • Name
      IVa2

Organism names

  • Taxonomic identifier
  • Organism
  • Strain
    • BGD/2004/B172/[P58/H10/F9]
  • Taxonomic lineage
    Viruses > Varidnaviria > Bamfordvirae > Preplasmiviricota > Tectiliviricetes > Rowavirales > Adenoviridae > Mastadenovirus > Human mastadenovirus D

Accessions

  • Primary accession
    H0PPF0

Proteomes

Subcellular Location

Virion
Host nucleus, host nucleoplasm
Host nucleus, host nucleolus
Note: Located at a unique vertex of the capsid. Present in about 6-8 copies per virion.

Keywords

PTM/Processing

Keywords

Expression

Induction

Expressed in the intermediate phase of the viral replicative cycle.

Interaction

Subunit

Homodimer. Part of a genome packaging complex composed of packaging proteins 1, 2 and 3; this complex specifically binds to the packaging sequence on the left end of viral genomic DNA and performs packaging of the viral genome. Interacts with protein 33K.

Family & Domains

Features

Showing features for region, compositional bias, domain.

Type
IDPosition(s)Description
Region1-77Disordered
Compositional bias32-50Basic and acidic residues
Compositional bias60-69Polar residues
Domain163-347AAA+ ATPase
Region440-448DNA-binding

Sequence similarities

Belongs to the adenoviridae packaging protein 1 family.

Family and domain databases

Sequence

  • Sequence status
    Complete
  • Length
    448
  • Mass (Da)
    50,622
  • Last updated
    2012-02-22 v1
  • MD5 Checksum
    82E85BA9E633D410FC18172E7215C969
METRGRRPCPFQHQQDESQAHPCKRPARGPPLHRDGDHAHADPETLEGHDAGSAGRPSSRALQSQSSQPPKRGSLLDRDAVEHVTELWDRLELLSQTLAKMPMADGLKPLKNFASLQELLSLGGDRLLGELVRENLQVRDMLNEVAPLLRDDGSCISLNYHLQPVIGVIYGPTGCGKSQLLRNLLSSQLITPAPETVFFIAPQVDMIPPSEMKAWEMQICEGNFAPGPEGTIVPQSGTLRPKFIKMSYDDLTQEHNYDVSDPRNVFAKAAAHGPIAIIMDECMENLGGHKGVSKFFHAFPSKLHDKFPKCTGYTVLVVLHNMNPRRDLGGNIANLKIQAKLHIISPRMHPSQLNRFANTYTKGLPVAISLLLKDIIQHHAQRPCYDWIIYNTTPEHEAMQWCYLHPRDGLMPMYLNIQSHLYRVLEKIHRTLNDRERWTRAYRARKNK

Features

Showing features for compositional bias.

TypeIDPosition(s)Description
Compositional bias32-50Basic and acidic residues
Compositional bias60-69Polar residues

Sequence databases

Nucleotide SequenceProtein SequenceMolecule TypeStatus
AP012285
EMBL· GenBank· DDBJ
BAL41711.1
EMBL· GenBank· DDBJ
Genomic DNA

Similar Proteins

Disclaimer

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