H0PPF0 · H0PPF0_9ADEN
- ProteinPackaging protein 1
- GeneIVa2
- StatusUniProtKB unreviewed (TrEMBL)
- Organism
- Amino acids448 (go to sequence)
- Protein existenceEvidence at transcript level
- Annotation score3/5
Function
function
Component of the packaging machinery which encapsidates the viral DNA into preformed capsids and transcriptional activator of the viral major late promoter (MLP). Binds, along with packaging proteins 2 and 3, to the specific packaging sequence on the left end of viral genomic DNA and displays ATPase activity thereby providing the power stroke of the packaging machinery. The activity of packaging protein IVa2 is stimulated by protein 33K which acts as a terminase. May be the protein that pumps DNA into the capsid powered by ATP hydrolysis. Specifically binds to the 5'-CG-3' nucleotides of the repeats making up the packaging sequence. Component of the DEF-A and DEF-B transcription factors that bind downstream elements of the major late promoter (MLP), and stimulate transcription from the MLP after initiation of viral DNA replication. DEF-A is a heterodimer packaging proteins 1 and 2 and DEF-B is a homodimer of packaging protein 1.
Features
Showing features for binding site.
Type | ID | Position(s) | Description | ||
---|---|---|---|---|---|
Binding site | 171-178 | ATP (UniProtKB | ChEBI) | |||
GO annotations
Aspect | Term | |
---|---|---|
Cellular Component | host cell nucleolus | |
Cellular Component | host cell nucleoplasm | |
Cellular Component | virion component | |
Molecular Function | ATP binding | |
Molecular Function | ATP hydrolysis activity | |
Molecular Function | DNA binding | |
Biological Process | DNA-templated transcription | |
Biological Process | nuclear capsid assembly | |
Biological Process | regulation of DNA-templated transcription | |
Biological Process | viral DNA genome packaging via site-specific sequence recognition | |
Biological Process | viral release from host cell | |
Biological Process | viral transcription |
Keywords
- Molecular function
- Biological process
- Ligand
Names & Taxonomy
Protein names
- Recommended namePackaging protein 1
- Alternative names
Gene names
Organism names
- Organism
- Strain
- Taxonomic lineageViruses > Varidnaviria > Bamfordvirae > Preplasmiviricota > Tectiliviricetes > Rowavirales > Adenoviridae > Mastadenovirus > Human mastadenovirus D
Accessions
- Primary accessionH0PPF0
Proteomes
Subcellular Location
UniProt Annotation
GO Annotation
Note: Located at a unique vertex of the capsid. Present in about 6-8 copies per virion.
Keywords
- Cellular component
PTM/Processing
Keywords
- PTM
Expression
Induction
Expressed in the intermediate phase of the viral replicative cycle.
Interaction
Subunit
Homodimer. Part of a genome packaging complex composed of packaging proteins 1, 2 and 3; this complex specifically binds to the packaging sequence on the left end of viral genomic DNA and performs packaging of the viral genome. Interacts with protein 33K.
Structure
Family & Domains
Features
Showing features for region, compositional bias, domain.
Type | ID | Position(s) | Description | ||
---|---|---|---|---|---|
Region | 1-77 | Disordered | |||
Compositional bias | 32-50 | Basic and acidic residues | |||
Compositional bias | 60-69 | Polar residues | |||
Domain | 163-347 | AAA+ ATPase | |||
Region | 440-448 | DNA-binding | |||
Sequence similarities
Belongs to the adenoviridae packaging protein 1 family.
Family and domain databases
Sequence
- Sequence statusComplete
- Length448
- Mass (Da)50,622
- Last updated2012-02-22 v1
- MD5 Checksum82E85BA9E633D410FC18172E7215C969
Features
Showing features for compositional bias.
Type | ID | Position(s) | Description | ||
---|---|---|---|---|---|
Compositional bias | 32-50 | Basic and acidic residues | |||
Compositional bias | 60-69 | Polar residues | |||
Sequence databases
Nucleotide Sequence | Protein Sequence | Molecule Type | Status | |
---|---|---|---|---|
AP012285 EMBL· GenBank· DDBJ | BAL41711.1 EMBL· GenBank· DDBJ | Genomic DNA |