G8H5M7 · TPS9_SOLHA

Function

function

Sesquiterpene synthase involved in the biosynthesis of volatile compounds (PubMed:21818683).
Mediates the conversion of (2E,6E)-farnesyl diphosphate (FPP) into (1E,4E)-germacrene B, but also smaller amounts of germacrene A and C, and of (2Z,6Z)-farnesyl diphosphate ((ZZ)-FPP) into alpha-humulene, germacrene A and germacrene B (PubMed:21818683).
Can act with a low efficiency as a monoterpene synthase with geranyl diphosphate (GPP) as substrate, thus producing beta-myrcene, limonene and terpinolene (PubMed:21818683).

Catalytic activity

  • (2E,6E)-farnesyl diphosphate = (1E,4E)-germacrene B + diphosphate
    This reaction proceeds in the forward direction.
    EC:4.2.3.71 (UniProtKB | ENZYME | Rhea)
  • (2E)-geranyl diphosphate = diphosphate + terpinolene
    This reaction proceeds in the forward direction.
    EC:4.2.3.113 (UniProtKB | ENZYME | Rhea)
  • (2E)-geranyl diphosphate = diphosphate + limonene
    This reaction proceeds in the forward direction.
  • (2E)-geranyl diphosphate = beta-myrcene + diphosphate
    This reaction proceeds in the forward direction.
    EC:4.2.3.15 (UniProtKB | ENZYME | Rhea)
  • (2Z,6Z)-farnesyl diphosphate = diphosphate + germacrene A
    This reaction proceeds in the forward direction.
  • (2Z,6Z)-farnesyl diphosphate = alpha-humulene + diphosphate
    This reaction proceeds in the forward direction.

Cofactor

Mg2+ (UniProtKB | Rhea| CHEBI:18420 )

Mn2+ (UniProtKB | Rhea| CHEBI:29035 )

Note: Binds 3 Mg2+ or Mn2+ ions per subunit.

Pathway

Secondary metabolite biosynthesis; terpenoid biosynthesis.

Features

Showing features for binding site.

TypeIDPosition(s)Description
Binding site296Mg2+ 1 (UniProtKB | ChEBI)
Binding site296Mg2+ 2 (UniProtKB | ChEBI)
Binding site300Mg2+ 1 (UniProtKB | ChEBI)
Binding site300Mg2+ 2 (UniProtKB | ChEBI)
Binding site449Mg2+ 3 (UniProtKB | ChEBI)

GO annotations

AspectTerm
Molecular Functionmagnesium ion binding
Molecular Functionterpene synthase activity
Biological Processditerpenoid biosynthetic process
Biological Processterpenoid biosynthetic process

Keywords

Enzyme and pathway databases

Names & Taxonomy

Protein names

  • Recommended name
    Terpene synthase 9
  • Short names
    ShTPS9
  • Alternative names
    • (1E,4E)-germacrene B synthase TPS9
      (EC:4.2.3.71
      ) . EC:4.2.3.71 (UniProtKB | ENZYME | Rhea)
    • Alpha-humulene synthase TPS9
      (EC:4.2.3.-
      ) . EC:4.2.3.- (UniProtKB | ENZYME | Rhea)
    • Beta-myrcene synthase TPS9
      (EC:4.2.3.15
      ) . EC:4.2.3.15 (UniProtKB | ENZYME | Rhea)
    • Germacrene A synthase TPS9
      (EC:4.2.3.-
      ) . EC:4.2.3.- (UniProtKB | ENZYME | Rhea)
    • Limonene synthase TPS9
      (EC:4.2.3.-
      ) . EC:4.2.3.- (UniProtKB | ENZYME | Rhea)
    • Terpinolene synthase TPS9
      (EC:4.2.3.113
      ) . EC:4.2.3.113 (UniProtKB | ENZYME | Rhea)

Gene names

    • Name
      TPS9

Organism names

  • Taxonomic identifier
  • Strain
    • cv. PI127826
  • Taxonomic lineage
    Eukaryota > Viridiplantae > Streptophyta > Embryophyta > Tracheophyta > Spermatophyta > Magnoliopsida > eudicotyledons > Gunneridae > Pentapetalae > asterids > lamiids > Solanales > Solanaceae > Solanoideae > Solaneae > Solanum > Solanum subgen. Lycopersicon

Accessions

  • Primary accession
    G8H5M7

PTM/Processing

Features

Showing features for chain.

TypeIDPosition(s)Description
ChainPRO_00004546821-544Terpene synthase 9

Structure

Family & Domains

Features

Showing features for motif.

TypeIDPosition(s)Description
Motif296-300DDXXD motif

Domain

The Asp-Asp-Xaa-Xaa-Asp/Glu (DDXXD/E) motif is important for the catalytic activity, presumably through binding to Mg2+.

Sequence similarities

Belongs to the terpene synthase family. Tpsa subfamily.

Family and domain databases

Sequence

  • Sequence status
    Complete
  • Length
    544
  • Mass (Da)
    63,794
  • Last updated
    2012-01-25 v1
  • Checksum
    832652FCE8B05477
MAASSANKSRPLANFHPTVWGYHFLSYTHEITNQEKVEVDEYKETIRKMLVEAPEGSEQKLVLIDAMQRLGVAYHFHNEIETSIQNIFDAPKQNNNLHIVSLHFRLVRQQGHYMSSDVFKQFTNQDGKFKERLTNDVQGLLSLYEASYLRVRDEEILEEALAFTTTHLKSIVSNMSNNNNSLKVEVSEALTQPIRMTLPRMEARRYISIYENNDAHNHLLLKFAKLDFNMLQKLHQRELSDLTRWWKDLDFANKYPYARDRLVECYFWILGVYFEPKYSRARKMMTKVLKMTSIIDDTFDAYATFDELEPFNDAIQRWDANAIDSIQPYMRPAYQAFLDIYSEMEQVLSKEGKLDRVYYAKNEMKKLVRAYFKETQWLNDCDHIPKYEEHMENSLVSGGYMMIPTTCLVGMEEFISIETFEWLMNDPLIVRASSLIARAMNDIVGHEVEQERGHVASLIECYMKDYGASKQEAYAKFKKDVTNAWKDINKEFFRPTEVPMFVLERVLNLTRAADTLYKEKDAYTNAKGKLKNMINSILIESVKI

Sequence databases

Nucleotide SequenceProtein SequenceMolecule TypeStatus
JN402388
EMBL· GenBank· DDBJ
AEM23825.1
EMBL· GenBank· DDBJ
mRNA

Similar Proteins

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