G8H5M7 · TPS9_SOLHA
- ProteinTerpene synthase 9
- GeneTPS9
- StatusUniProtKB reviewed (Swiss-Prot)
- Amino acids544 (go to sequence)
- Protein existenceEvidence at protein level
- Annotation score5/5
Function
function
Sesquiterpene synthase involved in the biosynthesis of volatile compounds (PubMed:21818683).
Mediates the conversion of (2E,6E)-farnesyl diphosphate (FPP) into (1E,4E)-germacrene B, but also smaller amounts of germacrene A and C, and of (2Z,6Z)-farnesyl diphosphate ((ZZ)-FPP) into alpha-humulene, germacrene A and germacrene B (PubMed:21818683).
Can act with a low efficiency as a monoterpene synthase with geranyl diphosphate (GPP) as substrate, thus producing beta-myrcene, limonene and terpinolene (PubMed:21818683).
Mediates the conversion of (2E,6E)-farnesyl diphosphate (FPP) into (1E,4E)-germacrene B, but also smaller amounts of germacrene A and C, and of (2Z,6Z)-farnesyl diphosphate ((ZZ)-FPP) into alpha-humulene, germacrene A and germacrene B (PubMed:21818683).
Can act with a low efficiency as a monoterpene synthase with geranyl diphosphate (GPP) as substrate, thus producing beta-myrcene, limonene and terpinolene (PubMed:21818683).
Catalytic activity
- (2E,6E)-farnesyl diphosphate = (1E,4E)-germacrene B + diphosphateThis reaction proceeds in the forward direction.
- (2E)-geranyl diphosphate = diphosphate + limoneneThis reaction proceeds in the forward direction.
- (2Z,6Z)-farnesyl diphosphate = diphosphate + germacrene AThis reaction proceeds in the forward direction.
- (2Z,6Z)-farnesyl diphosphate = alpha-humulene + diphosphateThis reaction proceeds in the forward direction.
Cofactor
Mn2+ (UniProtKB | Rhea| CHEBI:29035 )
Note: Binds 3 Mg2+ or Mn2+ ions per subunit.
Pathway
Secondary metabolite biosynthesis; terpenoid biosynthesis.
Features
Showing features for binding site.
GO annotations
Aspect | Term | |
---|---|---|
Molecular Function | magnesium ion binding | |
Molecular Function | terpene synthase activity | |
Biological Process | diterpenoid biosynthetic process | |
Biological Process | terpenoid biosynthetic process |
Keywords
- Molecular function
- Ligand
Enzyme and pathway databases
Names & Taxonomy
Protein names
- Recommended nameTerpene synthase 9
- Short namesShTPS9
- Alternative names
Gene names
Organism names
- Strain
- Taxonomic lineageEukaryota > Viridiplantae > Streptophyta > Embryophyta > Tracheophyta > Spermatophyta > Magnoliopsida > eudicotyledons > Gunneridae > Pentapetalae > asterids > lamiids > Solanales > Solanaceae > Solanoideae > Solaneae > Solanum > Solanum subgen. Lycopersicon
Accessions
- Primary accessionG8H5M7
PTM/Processing
Features
Showing features for chain.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Chain | PRO_0000454682 | 1-544 | Terpene synthase 9 | |||
Sequence: MAASSANKSRPLANFHPTVWGYHFLSYTHEITNQEKVEVDEYKETIRKMLVEAPEGSEQKLVLIDAMQRLGVAYHFHNEIETSIQNIFDAPKQNNNLHIVSLHFRLVRQQGHYMSSDVFKQFTNQDGKFKERLTNDVQGLLSLYEASYLRVRDEEILEEALAFTTTHLKSIVSNMSNNNNSLKVEVSEALTQPIRMTLPRMEARRYISIYENNDAHNHLLLKFAKLDFNMLQKLHQRELSDLTRWWKDLDFANKYPYARDRLVECYFWILGVYFEPKYSRARKMMTKVLKMTSIIDDTFDAYATFDELEPFNDAIQRWDANAIDSIQPYMRPAYQAFLDIYSEMEQVLSKEGKLDRVYYAKNEMKKLVRAYFKETQWLNDCDHIPKYEEHMENSLVSGGYMMIPTTCLVGMEEFISIETFEWLMNDPLIVRASSLIARAMNDIVGHEVEQERGHVASLIECYMKDYGASKQEAYAKFKKDVTNAWKDINKEFFRPTEVPMFVLERVLNLTRAADTLYKEKDAYTNAKGKLKNMINSILIESVKI |
Structure
Family & Domains
Features
Showing features for motif.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Motif | 296-300 | DDXXD motif | ||||
Sequence: DDTFD |
Domain
The Asp-Asp-Xaa-Xaa-Asp/Glu (DDXXD/E) motif is important for the catalytic activity, presumably through binding to Mg2+.
Sequence similarities
Belongs to the terpene synthase family. Tpsa subfamily.
Family and domain databases
Sequence
- Sequence statusComplete
- Length544
- Mass (Da)63,794
- Last updated2012-01-25 v1
- Checksum832652FCE8B05477