G7XMT1 · CYPE5_ASPKW

Function

function

Self-sufficient cytochrome P450 monooxygenase that catalyzes the regioselective in-chain hydroxylation of alkanes, fatty alcohols, and fatty acids at the omega-7 position (PubMed:36607403).
Performs hydroxylation of C10-C16 n-alkanes and C12 and C14 fatty alcohols; and thereby enables the one step biocatalytic synthesis of rare alcohols such as 5-dodecanol and 7-tetradecanol (PubMed:36607403).
Converts 1-dodecanol into 1,5-dodecanediol as major product with very little sub-terminally hydroxylated products with the 1,4-dodecanediol and 1,6-dodecanediol more abundant (PubMed:36607403).
Converts dodecanoic acid to 5-hydroxydodecanoic acid which can be further converted into delta-dodecalactone by lactonization of the 5-hydroxy acid at low pH (PubMed:36607403).
Also gives sub-terminal hydroxylation of dodecanoic acid with 9-hydroxydodecanoic acid being the second most abundant product (PubMed:36607403).

Catalytic activity

  • NADPH + 2 oxidized [cytochrome P450] = H+ + NADP+ + 2 reduced [cytochrome P450]
    EC:1.6.2.4 (UniProtKB | ENZYME | Rhea)
  • an organic molecule + O2 + reduced [NADPH--hemoprotein reductase] = an alcohol + H+ + H2O + oxidized [NADPH--hemoprotein reductase]
    EC:1.14.14.1 (UniProtKB | ENZYME | Rhea)
  • dodecanoate + O2 + reduced [NADPH--hemoprotein reductase] = 5-hydroxydodecanoate + H+ + H2O + oxidized [NADPH--hemoprotein reductase]
    This reaction proceeds in the forward direction.
  • O2 + reduced [NADPH--hemoprotein reductase] + tetradecanoate = 7-hydroxytetradecanoate + H+ + H2O + oxidized [NADPH--hemoprotein reductase]
    This reaction proceeds in the forward direction.
  • dodecan-1-ol + O2 + reduced [NADPH--hemoprotein reductase] = 1,5-dodecanediol + H+ + H2O + oxidized [NADPH--hemoprotein reductase]
    This reaction proceeds in the forward direction.
  • dodecan-1-ol + O2 + reduced [NADPH--hemoprotein reductase] = 1,4-dodecanediol + H+ + H2O + oxidized [NADPH--hemoprotein reductase]
    This reaction proceeds in the forward direction.
  • dodecan-1-ol + O2 + reduced [NADPH--hemoprotein reductase] = 1,6-dodecanediol + H+ + H2O + oxidized [NADPH--hemoprotein reductase]
    This reaction proceeds in the forward direction.

Cofactor

Protein has several cofactor binding sites:
FAD (UniProtKB | Rhea| CHEBI:57692 )

Note: Binds 1 FAD.
FMN (UniProtKB | Rhea| CHEBI:58210 )

Note: Binds 1 FMN.
heme (UniProtKB | Rhea| CHEBI:30413 )

Features

Showing features for binding site.

TypeIDPosition(s)Description
Binding site405Fe (UniProtKB | ChEBI) of heme (UniProtKB | ChEBI); axial binding residue
Binding site506-510FMN (UniProtKB | ChEBI)
Binding site585-617FMN (UniProtKB | ChEBI)

GO annotations

AspectTerm
Cellular Componentcytosol
Molecular Functionaromatase activity
Molecular Functionflavin adenine dinucleotide binding
Molecular FunctionFMN binding
Molecular Functionheme binding
Molecular Functioniron ion binding
Molecular FunctionNADPH-hemoprotein reductase activity

Keywords

Names & Taxonomy

Protein names

  • Recommended name
    Self-sufficient cytochrome P450 monooxygenase CYP505E5
  • Alternative names
    • Bifunctional cytochrome P450/NADPH--P450 reductase CYP505E5

Including 2 domains:

  • Recommended name
    Cytochrome P450 monooxygenase
  • EC number
  • Recommended name
    NADPH--cytochrome P450 reductase
  • EC number

Gene names

    • Name
      CYP505E5
    • ORF names
      AKAW_06479

Organism names

Accessions

  • Primary accession
    G7XMT1

Organism-specific databases

Subcellular Location

PTM/Processing

Features

Showing features for chain.

TypeIDPosition(s)Description
ChainPRO_00004590371-1050Self-sufficient cytochrome P450 monooxygenase CYP505E5

Interaction

Protein-protein interaction databases

Structure

Family & Domains

Features

Showing features for region, domain.

TypeIDPosition(s)Description
Region461-495Disordered
Domain500-641Flavodoxin-like
Domain679-907FAD-binding FR-type

Sequence similarities

In the N-terminal section; belongs to the cytochrome P450 family.

Phylogenomic databases

Family and domain databases

Sequence

  • Sequence status
    Complete
  • Length
    1,050
  • Mass (Da)
    116,590
  • Last updated
    2012-01-25 v1
  • Checksum
    96B287877AB891C4
MKDAERIPGPKPLPVVGNLFDIDPEHSLESIVAFAEKFGPLFQITINGEKQIFATSQALVDELCDELRFHKAVVTGLEILRLLAHDGLFTAYHGERGWGIAHRILVPAFGPLRIRNMLDDMSDVAQQLCLKWARQGGSTSINITEDFTRLTLDTIALCTMGFRLNSFYNNETMHPFVQSMLYVLREADIQANLPGIANSIRVSAQRRMHKNIEAMRTMARGIIQERRKNKNPVDDILNTLLNGRDPVTGEGMSDDSIIDNVITFLIAGHETTSGLLSFTFYFLIQHPHILKKAQEEVDETVGLAQISAQHLAELPYIDAILKESLRLMPTAPGFTVTPKKTEVLGGRWMINAGQPVNVLLPACLRDQSVFGPDADEFRPERMLAENFSKLPPNSWKPFGNGERGCIGRAFAWQEAQLVVAMILQTFDLVPDDPSYQLRIKETLTIKPDGFRIRATLRRGQTATGLSRRSMLVARDGSSEESSNHPAEARGDHAPARGQPVSFFYGSNSGTCKALAHQLASNMMSRGYTTQKLAPLDNAVDNLPRDQPVIILTTTYDGQPTDNAKKFVAWLETGNVLSLQGISYAVFGCGHHDWTQTFYRIPILIDDLMYKAGATRLAPRGAANAAVSDLFSDLEAWEETSLLPALRENFLPSNSTDFDPLNPHQIQLSLSKPRRVDLHKGLIEAKVTAVRVLTSPDTPEKRHLEFCFQGDLSLRPGDHLNILPVNPPSTVSRVLAQFNLAPDYNITVNSFNTLGLPQATPVSASELFSSYVELCQPATRNNLKSLIAATQSDTVKQELNRLYDSYEFIVRDKRVSVLDLLEQFPSISLPIAAFISMLPALRVRTYSLSMAPAFKPSHSSLTFSVINEPAWRGSGQHLGVASNYLASLTSGSIFYFSPRPAKETFHLPKDPSRTPIIMICAGSGLAPFLSFIQDRMVLKQQNKPLAKAFLFFGCRGRSLDDLYHEELSEYEAAGVVEVRRAYSKTPEFDIAKGCRYVQHRLVTEGQAILSLWAQNAIIYVCGSTSMAKGAEAVLQNMLGPLPKERYVTEIF

Sequence databases

Nucleotide SequenceProtein SequenceMolecule TypeStatus
DF126462
EMBL· GenBank· DDBJ
GAA88365.1
EMBL· GenBank· DDBJ
Genomic DNA

Similar Proteins

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