G7X8S6 · NIT2_ASPKW
- ProteinArylacetonitrilase
- Genenit2
- StatusUniProtKB reviewed (Swiss-Prot)
- Amino acids328 (go to sequence)
- Protein existenceEvidence at protein level
- Annotation score3/5
Function
function
Nitrilase that hydrolyzes preferentially phenylacetonitrile and (R,S)-mandelonitrile. Also acts on dinitriles like phenylenediacetonitriles (PDAs) 1,2-PDA, 1,3-PDA, and 1,4-PDA, and cyanophenyl acetonitriles (CPAs) 2-CPA and 4-CPA.
Catalytic activity
- a nitrile + 2 H2O = a carboxylate + NH4+
Features
Showing features for active site.
Type | ID | Position(s) | Description | ||
---|---|---|---|---|---|
Active site | 45 | Proton acceptor | |||
Active site | 125 | ||||
Active site | 160 | Nucleophile | |||
GO annotations
Aspect | Term | |
---|---|---|
Molecular Function | arylacetonitrilase activity | |
Molecular Function | indole-3-acetonitrile nitrilase activity |
Keywords
- Molecular function
Enzyme and pathway databases
Names & Taxonomy
Protein names
- Recommended nameArylacetonitrilase
- EC number
- Alternative names
Gene names
Organism names
- Strain
- Taxonomic lineageEukaryota > Fungi > Dikarya > Ascomycota > Pezizomycotina > Eurotiomycetes > Eurotiomycetidae > Eurotiales > Aspergillaceae > Aspergillus > Aspergillus subgen. Circumdati
Accessions
- Primary accessionG7X8S6
- Secondary accessions
Organism-specific databases
PTM/Processing
Features
Showing features for chain.
Type | ID | Position(s) | Description | ||
---|---|---|---|---|---|
Chain | PRO_0000451135 | 1-328 | Arylacetonitrilase | ||
Interaction
Protein-protein interaction databases
Structure
Family & Domains
Features
Showing features for domain.
Type | ID | Position(s) | Description | ||
---|---|---|---|---|---|
Domain | 5-278 | CN hydrolase | |||
Sequence similarities
Belongs to the carbon-nitrogen hydrolase superfamily. Nitrilase family.
Phylogenomic databases
Family and domain databases
Sequence
- Sequence statusComplete
- Length328
- Mass (Da)35,821
- Last updated2012-01-25 v1
- Checksum57E78101FBAE586C
Sequence databases
Nucleotide Sequence | Protein Sequence | Molecule Type | Status | |
---|---|---|---|---|
LN875496 EMBL· GenBank· DDBJ | CTQ87320.1 EMBL· GenBank· DDBJ | Genomic DNA | ||
DF126449 EMBL· GenBank· DDBJ | GAA83217.1 EMBL· GenBank· DDBJ | Genomic DNA |