G7W3K5 · G7W3K5_PAETH

Function

function

Specifically methylates position 2 of adenine 2503 in 23S rRNA and position 2 of adenine 37 in tRNAs.

Miscellaneous

Reaction proceeds by a ping-pong mechanism involving intermediate methylation of a conserved cysteine residue.

Caution

Lacks conserved residue(s) required for the propagation of feature annotation.

Catalytic activity

Cofactor

[4Fe-4S] cluster (UniProtKB | Rhea| CHEBI:49883 )

Note: Binds 1 [4Fe-4S] cluster. The cluster is coordinated with 3 cysteines and an exchangeable S-adenosyl-L-methionine.

Features

Showing features for active site, binding site.

Type
IDPosition(s)Description
Active site92Proton acceptor
Binding site112[4Fe-4S] cluster (UniProtKB | ChEBI); 4Fe-4S-S-AdoMet
Binding site116[4Fe-4S] cluster (UniProtKB | ChEBI); 4Fe-4S-S-AdoMet
Binding site119[4Fe-4S] cluster (UniProtKB | ChEBI); 4Fe-4S-S-AdoMet
Binding site164-165S-adenosyl-L-methionine (UniProtKB | ChEBI)
Binding site196S-adenosyl-L-methionine (UniProtKB | ChEBI)
Binding site219-221S-adenosyl-L-methionine (UniProtKB | ChEBI)
Binding site297S-adenosyl-L-methionine (UniProtKB | ChEBI)
Active site341S-methylcysteine intermediate

GO annotations

AspectTerm
Cellular Componentcytoplasm
Molecular Function4 iron, 4 sulfur cluster binding
Molecular Functionmetal ion binding
Molecular FunctionrRNA (adenine(2503)-C2-)-methyltransferase activity
Molecular FunctionrRNA binding
Molecular FunctiontRNA (adenine(37)-C2)-methyltransferase activity
Molecular FunctiontRNA binding
Biological ProcessrRNA base methylation
Biological ProcesstRNA methylation

Keywords

Enzyme and pathway databases

Names & Taxonomy

Protein names

  • Recommended name
    Probable dual-specificity RNA methyltransferase RlmN
  • EC number
  • Alternative names
    • 23S rRNA (adenine(2503)-C(2))-methyltransferase
    • 23S rRNA m2A2503 methyltransferase
    • Ribosomal RNA large subunit methyltransferase N
    • tRNA (adenine(37)-C(2))-methyltransferase
    • tRNA m2A37 methyltransferase

Gene names

    • Name
      rlmN
    • Ordered locus names
      HPL003_06645

Organism names

Accessions

  • Primary accession
    G7W3K5

Proteomes

Subcellular Location

Keywords

PTM/Processing

Keywords

Interaction

Protein-protein interaction databases

Family & Domains

Features

Showing features for domain.

TypeIDPosition(s)Description
Domain98-330Radical SAM core

Sequence similarities

Belongs to the radical SAM superfamily. RlmN family.

Phylogenomic databases

Family and domain databases

Sequence

  • Sequence status
    Complete
  • Length
    365
  • Mass (Da)
    41,342
  • Last updated
    2012-01-25 v1
  • MD5 Checksum
    CA2ADBDD70058CF9ADD3B17818F7F0AD
MKKESIYGLTLDQLTAWLIEHGHKKSRALQVWDALYRKRITDFAAMTEVHENCTRLLAEHFPIETLEEHVKQQSADGTVKFLFRLQDGNLIETVLMRHKFGLSVCVTTQVGCNIGCSFCASGLLKKSRDLSSGEIVEQIMKVQLYLDQERPGDRVSHVVVMGIGEPFDNFVNLSDFIRVIKDHKGLAIGPRHITVSTSGLADKIIEFADSDLHVNLAISLHAPNNETRTRIMKINRAIPIEELMQAIDYYLDKTNRRITLEYILLKDINDSKEHALELAELVGHRRNLANVNLIPYNPVDEHSQYQRSESESITGFYDILKKQGISCSVRLEHGVDIDAACGQLRSKQIRKDAKGSRNPEREAIG

Sequence databases

Nucleotide SequenceProtein SequenceMolecule TypeStatus
CP003107
EMBL· GenBank· DDBJ
AET58092.1
EMBL· GenBank· DDBJ
Genomic DNA

Genome annotation databases

Similar Proteins

Disclaimer

Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care. Our staff consists of biologists and biochemists that are not trained to give medical advice.
We'd like to inform you that we have updated our Privacy Notice to comply with Europe’s new General Data Protection Regulation (GDPR) that applies since 25 May 2018.
Help