G7QDV5 · G7QDV5_9BACT

Function

function

Bifunctional enzyme that catalyzes the epimerization of the S- and R-forms of NAD(P)HX and the dehydration of the S-form of NAD(P)HX at the expense of ADP, which is converted to AMP. This allows the repair of both epimers of NAD(P)HX, a damaged form of NAD(P)H that is a result of enzymatic or heat-dependent hydration.
Catalyzes the dehydration of the S-form of NAD(P)HX at the expense of ADP, which is converted to AMP. Together with NAD(P)HX epimerase, which catalyzes the epimerization of the S- and R-forms, the enzyme allows the repair of both epimers of NAD(P)HX, a damaged form of NAD(P)H that is a result of enzymatic or heat-dependent hydration.
Catalyzes the epimerization of the S- and R-forms of NAD(P)HX, a damaged form of NAD(P)H that is a result of enzymatic or heat-dependent hydration. This is a prerequisite for the S-specific NAD(P)H-hydrate dehydratase to allow the repair of both epimers of NAD(P)HX.

Caution

Lacks conserved residue(s) required for the propagation of feature annotation.

Catalytic activity

Cofactor

Protein has several cofactor binding sites:
K+ (UniProtKB | Rhea| CHEBI:29103 )

Note: Binds 1 potassium ion per subunit.
Mg2+ (UniProtKB | Rhea| CHEBI:18420 )

Features

Showing features for binding site.

TypeIDPosition(s)Description
Binding site67-71(6S)-NADPHX (UniProtKB | ChEBI)
Binding site68K+ (UniProtKB | ChEBI)
Binding site134K+ (UniProtKB | ChEBI)
Binding site138-144(6S)-NADPHX (UniProtKB | ChEBI)
Binding site169(6S)-NADPHX (UniProtKB | ChEBI)
Binding site172K+ (UniProtKB | ChEBI)
Binding site270(6S)-NADPHX (UniProtKB | ChEBI)
Binding site339(6S)-NADPHX (UniProtKB | ChEBI)
Binding site389(6S)-NADPHX (UniProtKB | ChEBI)
Binding site426-430AMP (UniProtKB | ChEBI)
Binding site455AMP (UniProtKB | ChEBI)
Binding site456(6S)-NADPHX (UniProtKB | ChEBI)

GO annotations

AspectTerm
Molecular FunctionADP-dependent NAD(P)H-hydrate dehydratase activity
Molecular FunctionATP binding
Molecular Functionmetal ion binding
Molecular FunctionNADHX epimerase activity
Molecular FunctionNADPHX epimerase activity
Biological Processmetabolite repair
Biological Processnicotinamide nucleotide metabolic process

Keywords

Names & Taxonomy

Protein names

  • Recommended name
    Bifunctional NAD(P)H-hydrate repair enzyme
  • Alternative names
    • Nicotinamide nucleotide repair protein

Including 2 domains:

  • Recommended name
    ADP-dependent (S)-NAD(P)H-hydrate dehydratase
  • EC number
  • Alternative names
    • ADP-dependent NAD(P)HX dehydratase
  • Recommended name
    NAD(P)H-hydrate epimerase
  • EC number

Gene names

    • Name
      nnrE
    • Synonyms
      nnrD
    • ORF names
      DFW101_0594

Organism names

Accessions

  • Primary accession
    G7QDV5

Proteomes

Interaction

Subunit

Homotetramer.

Protein-protein interaction databases

Family & Domains

Features

Showing features for domain.

TypeIDPosition(s)Description
Domain17-226YjeF N-terminal
Domain235-514YjeF C-terminal

Sequence similarities

Belongs to the NnrD/CARKD family.
Belongs to the NnrE/AIBP family.
In the C-terminal section; belongs to the NnrD/CARKD family.
In the N-terminal section; belongs to the NnrE/AIBP family.

Phylogenomic databases

Family and domain databases

Sequence

  • Sequence status
    Complete
  • Length
    514
  • Mass (Da)
    52,549
  • Last updated
    2012-01-25 v1
  • Checksum
    E7C1896CDABC6820
MLQDERYFDPLPTPAEMTAWDAASVREGGLSFLALMENASREAMHALVGEVGDVRGKSVLLLAGPGNNGGDAVALARHLDGRGARVTIGLYRPRGRYKGAAGYNARVATRLGLAMAPLGKVDFSRADPPDILVDGLLGTGFAGELRPDMAAVVEKINTLRDRTFVFSLDIPSGLSGETGRPQPVAVTAHATVTFEAAKTGLVTAEAAPYVGKLLVRDIGIPRLVQEKHPCRAWRIAPGIKDALPAVSPTLHKGSAGRVVVVGGSRGLTGAPLLAALGALRSGAGLVSVACPGATEVALKAGYPDVMTMPMGEGGHFGPGDAAAVRQFVHGADAVALGPGLGRHPDTAGFLDALLPLPCRLVVDADGLYFLAQNPDLIPKLGPETVITPHPGEAARLLGIDIPAVEADRLASARALATRYGCVAVLKGPATVVAASDGQVAVSPVVAPNLAVGGSGDVLSGLTANLLSRPLSPLLAACLAVYWHGLGGARLAALYPIRGNLASEIAAMLPGALTE

Keywords

Sequence databases

Nucleotide SequenceProtein SequenceMolecule TypeStatus
CM001368
EMBL· GenBank· DDBJ
EHJ46611.1
EMBL· GenBank· DDBJ
Genomic DNA

Similar Proteins

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