G7LJ94 · G7LJ94_MEDTR
- ProteinATP-dependent 6-phosphofructokinase
- Gene11423706
- StatusUniProtKB unreviewed (TrEMBL)
- Amino acids529 (go to sequence)
- Protein existenceInferred from homology
- Annotation score3/5
Function
function
Catalyzes the phosphorylation of D-fructose 6-phosphate to fructose 1,6-bisphosphate by ATP, the first committing step of glycolysis.
Catalytic activity
- beta-D-fructose 6-phosphate + ATP = beta-D-fructose 1,6-bisphosphate + ADP + H+
Cofactor
Activity regulation
Allosterically activated by AMP.
Pathway
Carbohydrate degradation; glycolysis; D-glyceraldehyde 3-phosphate and glycerone phosphate from D-glucose: step 3/4.
Features
Showing features for binding site, site, active site.
Type | ID | Position(s) | Description | ||
---|---|---|---|---|---|
Binding site | 181 | ATP (UniProtKB | ChEBI) | |||
Binding site | 245-246 | ATP (UniProtKB | ChEBI) | |||
Binding site | 270-273 | ATP (UniProtKB | ChEBI) | |||
Binding site | 271 | Mg2+ (UniProtKB | ChEBI); catalytic | |||
Site | 272 | Important for substrate specificity; cannot use PPi as phosphoryl donor | |||
Binding site | 299-301 | substrate | |||
Active site | 301 | Proton acceptor | |||
Binding site | 344-346 | substrate | |||
Binding site | 400 | substrate | |||
Binding site | 452-455 | substrate | |||
GO annotations
Aspect | Term | |
---|---|---|
Cellular Component | cytoplasm | |
Molecular Function | 6-phosphofructokinase activity | |
Molecular Function | ATP binding | |
Molecular Function | metal ion binding | |
Biological Process | fructose 6-phosphate metabolic process |
Keywords
- Molecular function
- Biological process
- Ligand
Enzyme and pathway databases
Names & Taxonomy
Protein names
- Recommended nameATP-dependent 6-phosphofructokinase
- EC number
- Short namesATP-PFK ; Phosphofructokinase
- Alternative names
Gene names
Organism names
- Strains
- Taxonomic lineageEukaryota > Viridiplantae > Streptophyta > Embryophyta > Tracheophyta > Spermatophyta > Magnoliopsida > eudicotyledons > Gunneridae > Pentapetalae > rosids > fabids > Fabales > Fabaceae > Papilionoideae > 50 kb inversion clade > NPAAA clade > Hologalegina > IRL clade > Trifolieae > Medicago
Accessions
- Primary accessionG7LJ94
Proteomes
Genome annotation databases
Subcellular Location
PTM/Processing
Proteomic databases
Interaction
Structure
Family & Domains
Features
Showing features for domain.
Type | ID | Position(s) | Description | ||
---|---|---|---|---|---|
Domain | 174-476 | Phosphofructokinase | |||
Sequence similarities
Belongs to the phosphofructokinase type A (PFKA) family. PPi-dependent PFK group II subfamily. Atypical ATP-dependent clade 'X' sub-subfamily.
Phylogenomic databases
Family and domain databases
Sequence
- Sequence statusComplete
- Length529
- Mass (Da)58,138
- Last updated2012-01-25 v1
- ChecksumA3419391CD3C4DF8
Keywords
- Technical term
Sequence databases
Nucleotide Sequence | Protein Sequence | Molecule Type | Status | |
---|---|---|---|---|
CM001224 EMBL· GenBank· DDBJ | AET03390.1 EMBL· GenBank· DDBJ | Genomic DNA | ||
PSQE01000008 EMBL· GenBank· DDBJ | RHN41591.1 EMBL· GenBank· DDBJ | Genomic DNA |