G7KDA1 · PHT18_MEDTR
- ProteinLow affinity inorganic phosphate transporter 8
- GenePT8
- StatusUniProtKB reviewed (Swiss-Prot)
- Amino acids557 (go to sequence)
- Protein existenceEvidence at transcript level
- Annotation score5/5
Function
function
Low-affinity transporter for external inorganic phosphate (Pi) that may be involved in the acquisition of phosphate released by arbuscular mycorrhizal (AM) fungi (e.g. Glomus versiforme and G.intraradices) during AM symbiosis; not required for mycorrhizal arbuscule development.
Miscellaneous
Although related to the sugar transporter family, it does not transport sugars.
Catalytic activity
- H+(in) + phosphate(in) = H+(out) + phosphate(out)This reaction proceeds in the backward direction.H+ (in)CHEBI:15378
+ phosphate (in)CHEBI:43474= H+ (out)CHEBI:15378+ phosphate (out)CHEBI:43474
GO annotations
all annotations | all molecular function | nucleotide binding | molecular_function | nucleic acid binding | dna binding | chromatin binding | dna-binding transcription factor activity | rna binding | cytoskeletal motor activity | catalytic activity | nuclease activity | signaling receptor binding | structural molecule activity | transporter activity | binding | protein binding | translation factor activity, rna binding | lipid binding | kinase activity | transferase activity | hydrolase activity | oxygen binding | enzyme regulator activity | carbohydrate binding | signaling receptor activity | translation regulator activity | transcription regulator activity | other molecular function | all biological process | carbohydrate metabolic process | generation of precursor metabolites and energy | nucleobase-containing compound metabolic process | dna metabolic process | translation | lipid metabolic process | transport | response to stress | cell cycle | cell communication | signal transduction | cell-cell signaling | multicellular organism development | circadian rhythm | biological_process | metabolic process | catabolic process | biosynthetic process | response to light stimulus | response to external stimulus | tropism | response to biotic stimulus | response to abiotic stimulus | response to endogenous stimulus | embryo development | post-embryonic development | fruit ripening | abscission | pollination | flower development | cellular process | programmed cell death | photosynthesis | cellular component organization | cell growth | protein metabolic process | cellular homeostasis | secondary metabolic process | reproductive process | cell differentiation | protein modification process | growth | epigenetic regulation of gene expression | response to chemical | anatomical structure development | regulation of molecular function | other biological process | all cellular component | cellular_component | extracellular region | cell wall | intracellular anatomical structure | nucleus | nuclear envelope | nucleoplasm | nucleolus | cytoplasm | mitochondrion | lysosome | endosome | vacuole | peroxisome | endoplasmic reticulum | golgi apparatus | cytosol | ribosome | cytoskeleton | plasma membrane | chloroplast | plastid | thylakoid | membrane | external encapsulating structure | other cellular component | |||
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Aspect | Term | |
---|---|---|
Cellular Component | periarbuscular membrane | |
Cellular Component | plasma membrane | |
Molecular Function | hydrolase activity | |
Molecular Function | symporter activity | |
Biological Process | arbuscular mycorrhizal association | |
Biological Process | cellular response to nitrogen levels | |
Biological Process | phosphate ion transport | |
Biological Process | response to symbiotic fungus | |
Biological Process | transmembrane transport |
Keywords
- Molecular function
- Biological process
Names & Taxonomy
Protein names
- Recommended nameLow affinity inorganic phosphate transporter 8
- Short namesMtPT8 ; MtPht1;8
- Alternative names
Gene names
Organism names
- Strain
- Taxonomic lineageEukaryota > Viridiplantae > Streptophyta > Embryophyta > Tracheophyta > Spermatophyta > Magnoliopsida > eudicotyledons > Gunneridae > Pentapetalae > rosids > fabids > Fabales > Fabaceae > Papilionoideae > 50 kb inversion clade > NPAAA clade > Hologalegina > IRL clade > Trifolieae > Medicago
Accessions
- Primary accessionG7KDA1
Proteomes
Genome annotation databases
Subcellular Location
UniProt Annotation
GO Annotation
Cell membrane ; Multi-pass membrane protein
Note: Present on the periarbuscular membrane in cells containing arbuscules during arbuscular mycorrhizal (AM) symbiosis with AM fungi.
Features
Showing features for topological domain, transmembrane.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Topological domain | 1-20 | Cytoplasmic | ||||
Sequence: MATSHGVLRSLDNAKTQSYH | ||||||
Transmembrane | 21-41 | Helical | ||||
Sequence: YLAIVIAGMGFFTDAYDLFCI | ||||||
Topological domain | 42-70 | Extracellular | ||||
Sequence: TAVTKLIGRLYYSDPTNHSPGILPTNVNN | ||||||
Transmembrane | 71-91 | Helical | ||||
Sequence: AITGVALCGTLAGQLFFGWLG | ||||||
Topological domain | 92-98 | Cytoplasmic | ||||
Sequence: DKLGRKK | ||||||
Transmembrane | 99-119 | Helical | ||||
Sequence: VYGITLTTMVGFALLSGLSFG | ||||||
Topological domain | 120-130 | Extracellular | ||||
Sequence: STPKTVVTSLC | ||||||
Transmembrane | 131-151 | Helical | ||||
Sequence: FFRFWLGFGIGGDYPLSAVIM | ||||||
Topological domain | 152-162 | Cytoplasmic | ||||
Sequence: SEYANQKTRGS | ||||||
Transmembrane | 163-183 | Helical | ||||
Sequence: FIAAVFAMQGVGILVAGGVAM | ||||||
Topological domain | 184-210 | Extracellular | ||||
Sequence: FVSKLFLLYFPAPDFETDAVLSTQPEG | ||||||
Transmembrane | 211-231 | Helical | ||||
Sequence: DFVWRIVLMFGAVPAALTYYW | ||||||
Topological domain | 232-294 | Cytoplasmic | ||||
Sequence: RMKMPETARYTALVEGDHKKAVEDMAKVLDRNILSEESNTRIAIRPLESHSYGLFSSEFLNRH | ||||||
Transmembrane | 295-315 | Helical | ||||
Sequence: GLHLLGTTSTWFLLDIAFYSL | ||||||
Topological domain | 316-346 | Extracellular | ||||
Sequence: QLTQKDIYPTSGLVYKASKMNAIEEVFQLSR | ||||||
Transmembrane | 347-367 | Helical | ||||
Sequence: AMFAVALIATVPGYWCTVFLI | ||||||
Topological domain | 368-369 | Cytoplasmic | ||||
Sequence: EK | ||||||
Transmembrane | 370-390 | Helical | ||||
Sequence: IGRFRIQLIGFLVMSVCMWFL | ||||||
Topological domain | 391-414 | Extracellular | ||||
Sequence: GHNYRSFRGEESACKNGSKYSFCN | ||||||
Transmembrane | 415-435 | Helical | ||||
Sequence: GNPVMFAILFGLTLFFANFGP | ||||||
Topological domain | 436-457 | Cytoplasmic | ||||
Sequence: NSTTFIVPAELFPARLRSTCHG | ||||||
Transmembrane | 458-478 | Helical | ||||
Sequence: ISAAAGKSGAIVGAFGVQSYI | ||||||
Topological domain | 479-490 | Extracellular | ||||
Sequence: GNSHDKSKGTKQ | ||||||
Transmembrane | 491-511 | Helical | ||||
Sequence: AIMALAVVNLLGFFFTFLVPE | ||||||
Topological domain | 512-557 | Cytoplasmic | ||||
Sequence: TQGRSLEEISGEEKDFQGNNADEEISGERNGTRNASVDKSPETSMV |
Keywords
- Cellular component
Phenotypes & Variants
Disruption phenotype
Normal arbuscular mycorrhizal (AM) symbiosis with AM fungi (PubMed:25841038).
Plants missing both PT4 and PT8 fail to establish AM symbiosis with AM fungi in high nitrogen conditions, leading to premature arbuscule degeneration (PAD); these phenotypes are suppressed in nitrogen-deprived conditions in an AMT2-3-dependent manner (PubMed:25841038).
Plants missing both PT4 and PT8 fail to establish AM symbiosis with AM fungi in high nitrogen conditions, leading to premature arbuscule degeneration (PAD); these phenotypes are suppressed in nitrogen-deprived conditions in an AMT2-3-dependent manner (PubMed:25841038).
PTM/Processing
Features
Showing features for chain, glycosylation.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Chain | PRO_0000450033 | 1-557 | Low affinity inorganic phosphate transporter 8 | |||
Sequence: MATSHGVLRSLDNAKTQSYHYLAIVIAGMGFFTDAYDLFCITAVTKLIGRLYYSDPTNHSPGILPTNVNNAITGVALCGTLAGQLFFGWLGDKLGRKKVYGITLTTMVGFALLSGLSFGSTPKTVVTSLCFFRFWLGFGIGGDYPLSAVIMSEYANQKTRGSFIAAVFAMQGVGILVAGGVAMFVSKLFLLYFPAPDFETDAVLSTQPEGDFVWRIVLMFGAVPAALTYYWRMKMPETARYTALVEGDHKKAVEDMAKVLDRNILSEESNTRIAIRPLESHSYGLFSSEFLNRHGLHLLGTTSTWFLLDIAFYSLQLTQKDIYPTSGLVYKASKMNAIEEVFQLSRAMFAVALIATVPGYWCTVFLIEKIGRFRIQLIGFLVMSVCMWFLGHNYRSFRGEESACKNGSKYSFCNGNPVMFAILFGLTLFFANFGPNSTTFIVPAELFPARLRSTCHGISAAAGKSGAIVGAFGVQSYIGNSHDKSKGTKQAIMALAVVNLLGFFFTFLVPETQGRSLEEISGEEKDFQGNNADEEISGERNGTRNASVDKSPETSMV | ||||||
Glycosylation | 406 | N-linked (GlcNAc...) asparagine | ||||
Sequence: N |
Keywords
- PTM
Proteomic databases
PTM databases
Expression
Induction
Accumulates in roots during colonization by arbuscular mycorrhizal (AM) fungi (e.g. Glomus versiforme).
Developmental stage
During arbuscular mycorrhizal (AM) symbiosis with AM fungi, accumulates exclusively in cortical cells harboring arbuscules.
Interaction
Protein-protein interaction databases
Structure
Family & Domains
Features
Showing features for compositional bias, region.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Compositional bias | 519-540 | Basic and acidic residues | ||||
Sequence: EISGEEKDFQGNNADEEISGER | ||||||
Region | 519-557 | Disordered | ||||
Sequence: EISGEEKDFQGNNADEEISGERNGTRNASVDKSPETSMV | ||||||
Compositional bias | 541-557 | Polar residues | ||||
Sequence: NGTRNASVDKSPETSMV |
Sequence similarities
Keywords
- Domain
Phylogenomic databases
Family and domain databases
Sequence
- Sequence statusComplete
- Length557
- Mass (Da)61,025
- Last updated2012-01-25 v1
- Checksum14BE9379B97298F3
Features
Showing features for compositional bias.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Compositional bias | 519-540 | Basic and acidic residues | ||||
Sequence: EISGEEKDFQGNNADEEISGER | ||||||
Compositional bias | 541-557 | Polar residues | ||||
Sequence: NGTRNASVDKSPETSMV |
Keywords
- Technical term
Sequence databases
Nucleotide Sequence | Protein Sequence | Molecule Type | Status | |
---|---|---|---|---|
CM001221 EMBL· GenBank· DDBJ | AES98403.1 EMBL· GenBank· DDBJ | Genomic DNA | ||
PSQE01000005 EMBL· GenBank· DDBJ | RHN56345.1 EMBL· GenBank· DDBJ | Genomic DNA |