G7JVE7 · G7JVE7_MEDTR
- ProteinNADPH--cytochrome P450 reductase
- Gene11424724
- StatusUniProtKB unreviewed (TrEMBL)
- Amino acids701 (go to sequence)
- Protein existenceEvidence at transcript level
- Annotation score3/5
Function
function
This enzyme is required for electron transfer from NADP to cytochrome P450 in microsomes. It can also provide electron transfer to heme oxygenase and cytochrome B5.
Catalytic activity
- NADPH + 2 oxidized [cytochrome P450] = H+ + NADP+ + 2 reduced [cytochrome P450]
Cofactor
Protein has several cofactor binding sites:
Note: Binds 1 FAD per monomer.
Note: Binds 1 FMN per monomer.
Features
Showing features for binding site.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Binding site | 100-105 | FMN (UniProtKB | ChEBI) | ||||
Sequence: TQTGTA | ||||||
Binding site | 155-158 | FMN (UniProtKB | ChEBI) | ||||
Sequence: ATYG | ||||||
Binding site | 193-202 | FMN (UniProtKB | ChEBI) | ||||
Sequence: LGNRQYEHFN | ||||||
Binding site | 228 | FMN (UniProtKB | ChEBI) | ||||
Sequence: D | ||||||
Binding site | 319 | NADP+ (UniProtKB | ChEBI) | ||||
Sequence: R | ||||||
Binding site | 479-482 | FAD (UniProtKB | ChEBI) | ||||
Sequence: RYYS | ||||||
Binding site | 497-499 | FAD (UniProtKB | ChEBI) | ||||
Sequence: TCA | ||||||
Binding site | 513-516 | FAD (UniProtKB | ChEBI) | ||||
Sequence: GVCS | ||||||
Binding site | 560 | NADP+ (UniProtKB | ChEBI) | ||||
Sequence: T | ||||||
Binding site | 621-622 | NADP+ (UniProtKB | ChEBI) | ||||
Sequence: SR | ||||||
Binding site | 627-631 | NADP+ (UniProtKB | ChEBI) | ||||
Sequence: KEYVQ | ||||||
Binding site | 663 | NADP+ (UniProtKB | ChEBI) | ||||
Sequence: D | ||||||
Binding site | 701 | FAD (UniProtKB | ChEBI) | ||||
Sequence: W |
GO annotations
Aspect | Term | |
---|---|---|
Cellular Component | cytosol | |
Cellular Component | endoplasmic reticulum membrane | |
Molecular Function | flavin adenine dinucleotide binding | |
Molecular Function | FMN binding | |
Molecular Function | NADP binding | |
Molecular Function | NADPH-hemoprotein reductase activity |
Keywords
- Molecular function
- Ligand
Enzyme and pathway databases
Names & Taxonomy
Protein names
- Recommended nameNADPH--cytochrome P450 reductase
- EC number
- Short namesCPR ; P450R
Gene names
Organism names
- Strains
- Taxonomic lineageEukaryota > Viridiplantae > Streptophyta > Embryophyta > Tracheophyta > Spermatophyta > Magnoliopsida > eudicotyledons > Gunneridae > Pentapetalae > rosids > fabids > Fabales > Fabaceae > Papilionoideae > 50 kb inversion clade > NPAAA clade > Hologalegina > IRL clade > Trifolieae > Medicago
Accessions
- Primary accessionG7JVE7
Proteomes
Genome annotation databases
Subcellular Location
UniProt Annotation
GO Annotation
Endoplasmic reticulum membrane ; Single-pass membrane protein
Features
Showing features for transmembrane.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Transmembrane | 41-61 | Helical | ||||
Sequence: FVMILTTSIAVLIGCVVVLIW |
Keywords
- Cellular component
PTM/Processing
Proteomic databases
Interaction
Protein-protein interaction databases
Structure
Family & Domains
Features
Showing features for domain.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Domain | 94-244 | Flavodoxin-like | ||||
Sequence: VTVFFGTQTGTAEGFAKAIAEEAKARYEKAKFKVVDMDDYAADDDEYEEKLKKETMALFFLATYGDGEPTDNAARFYKWFEEFEGEEDSFKNLQYGVFGLGNRQYEHFNKVAKIVDDKLLEKGGNRLVPVGLGDDDQCIEDDFTAWKEELW | ||||||
Domain | 299-546 | FAD-binding FR-type | ||||
Sequence: HHPVRANVAVRKELHTPASDRSCTHLEFDISGTGVVYETGDHVGVYCENLSDTVEEAERILGLSPDTYFSVHTDDEDGKPLGGSSLPPPFPPCTLRTALAKYADVLSSPKKSALLALAAHASDPSEADRLRHLASPAGKDEYAEWVIASQRSLLEVMAEFSSAKPPIGVFFASVAPRLQPRYYSISSSPRVAPSRIHVTCALVHDKMPTGRIHQGVCSTWMKNSAPLEKSQDCSWAPIFVRQSNFRLP |
Sequence similarities
Belongs to the NADPH--cytochrome P450 reductase family.
In the C-terminal section; belongs to the flavoprotein pyridine nucleotide cytochrome reductase family.
In the N-terminal section; belongs to the flavodoxin family.
Keywords
- Domain
Phylogenomic databases
Family and domain databases
Sequence
- Sequence statusComplete
- Length701
- Mass (Da)77,974
- Last updated2012-01-25 v1
- Checksum9C42A03CDE7A1E4E
Keywords
- Technical term
Sequence databases
Nucleotide Sequence | Protein Sequence | Molecule Type | Status | |
---|---|---|---|---|
CM001220 EMBL· GenBank· DDBJ | AES92306.1 EMBL· GenBank· DDBJ | Genomic DNA | ||
KY798118 EMBL· GenBank· DDBJ | AUG98242.1 EMBL· GenBank· DDBJ | mRNA | ||
PSQE01000004 EMBL· GenBank· DDBJ | RHN64738.1 EMBL· GenBank· DDBJ | Genomic DNA |