G6EZT0 · G6EZT0_9PROT

Function

function

DNA ligase that catalyzes the formation of phosphodiester linkages between 5'-phosphoryl and 3'-hydroxyl groups in double-stranded DNA using NAD as a coenzyme and as the energy source for the reaction. It is essential for DNA replication and repair of damaged DNA.

Caution

Lacks conserved residue(s) required for the propagation of feature annotation.
The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.

Catalytic activity

  • NAD+ + (deoxyribonucleotide)n-3'-hydroxyl + 5'-phospho-(deoxyribonucleotide)m = (deoxyribonucleotide)n+m + AMP + beta-nicotinamide D-nucleotide.
    EC:6.5.1.2 (UniProtKB | ENZYME | Rhea)

Cofactor

Mg2+ (UniProtKB | Rhea| CHEBI:18420 )

Mn2+ (UniProtKB | Rhea| CHEBI:29035 )

Features

Showing features for binding site, active site.

TypeIDPosition(s)Description
Binding site49-53NAD+ (UniProtKB | ChEBI)
Binding site98-99NAD+ (UniProtKB | ChEBI)
Binding site134NAD+ (UniProtKB | ChEBI)
Active site136N6-AMP-lysine intermediate
Binding site157NAD+ (UniProtKB | ChEBI)
Binding site193NAD+ (UniProtKB | ChEBI)
Binding site309NAD+ (UniProtKB | ChEBI)
Binding site333NAD+ (UniProtKB | ChEBI)
Binding site428Zn2+ (UniProtKB | ChEBI)
Binding site431Zn2+ (UniProtKB | ChEBI)
Binding site452Zn2+ (UniProtKB | ChEBI)

GO annotations

AspectTerm
Molecular FunctionDNA ligase (NAD+) activity
Molecular Functionmetal ion binding
Biological ProcessDNA repair
Biological ProcessDNA replication

Keywords

Enzyme and pathway databases

Names & Taxonomy

Protein names

  • Recommended name
    DNA ligase
  • EC number
  • Alternative names
    • Polydeoxyribonucleotide synthase [NAD(+)]

Gene names

    • Name
      ligA
    • ORF names
      CIN_00850

Organism names

Accessions

  • Primary accession
    G6EZT0

Proteomes

Interaction

Protein-protein interaction databases

Family & Domains

Features

Showing features for domain.

TypeIDPosition(s)Description
Domain620-692BRCT

Sequence similarities

Belongs to the NAD-dependent DNA ligase family. LigA subfamily.

Phylogenomic databases

Family and domain databases

Sequence

  • Sequence status
    Complete
  • Length
    704
  • Mass (Da)
    78,730
  • Last updated
    2012-01-25 v1
  • Checksum
    AFB77A2085229932
MNSFQTPFSTIAFQDLTLDQAKEELSTLSDYLHQCDDAYYNQDNPVADDATYDQLRRRHKEIEAHFPELIEQNSPSQTVGSQPTSGLNKIKHRVPMLSLDNVFNGEEFQDFINRIQRYLDLKLHDLEHFTFVAEPKIDGLSINLTYEKGILTQASTRGDGTVGEDVTANIKTIASLPQQLTGNVPDVMEIRGEVYISKENFLKLNQEQEQANKRIFANPRNAAAGSLRQLDPEVTRARPLSLFVYAQGYSSQSIADTHDHFLKKLEAWGFDVNPLYQVVKNADEAEKFQQDLMTKRSGLPYDIDGIVYKVNTIALQNRLGFIGRSPRWATAWKFPAEQAITRLKKIDIQVGRTGALTPVALLEPINVGGVIVTRATLHNEDEIIRKDVRENDLVILQRAGDVIPQIVGVDPSTLETPRSEPYQFPTHCPVCGAHAEKPANEVVRRCTGGLTCNAQIEERLIHFCSKDAFDIEGMGNKTVSEFFHEGLIHQPADIFHLQNHQEKILHAEGWAELSLKNLLAAIENKKTISLDRFIYALGIRRIGIATAKVLAKHYTSYTNWGEQMLAARQIGSDERLTLGSIEGIGPAIADELVAFFSESHNQQALKDLHDVLNIQDVETTQEGILVGKILVFTGTLSTMTRSEAKDIAERLGAKVTSSVSAKTDYVIEGVDGGSKARKAKELNIKCLDEKEWRALIGYPEINEV

Sequence databases

Nucleotide SequenceProtein SequenceMolecule TypeStatus
AGFR01000003
EMBL· GenBank· DDBJ
EHD14153.1
EMBL· GenBank· DDBJ
Genomic DNA

Genome annotation databases

Similar Proteins

Disclaimer

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