G6EI76 · G6EI76_9SPHN

Function

function

APS kinase catalyzes the synthesis of activated sulfate.
Catalyzes the synthesis of activated sulfate.
Proposed to provide activated sulfate for transfer to Nod factor. ATP sulfurylase may be the GTPase, regulating ATP sulfurylase activity.
With CysD forms the ATP sulfurylase (ATPS) that catalyzes the adenylation of sulfate producing adenosine 5'-phosphosulfate (APS) and diphosphate, the first enzymatic step in sulfur assimilation pathway. APS synthesis involves the formation of a high-energy phosphoric-sulfuric acid anhydride bond driven by GTP hydrolysis by CysN coupled to ATP hydrolysis by CysD.

Caution

The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.

Catalytic activity

Pathway

Sulfur metabolism; hydrogen sulfide biosynthesis; sulfite from sulfate: step 1/3.
Sulfur metabolism; hydrogen sulfide biosynthesis; sulfite from sulfate: step 2/3.

Features

Showing features for binding site, active site.

Type
IDPosition(s)Description
Binding site41-48GTP (UniProtKB | ChEBI)
Binding site120-124GTP (UniProtKB | ChEBI)
Binding site175-178GTP (UniProtKB | ChEBI)
Binding site478-485ATP (UniProtKB | ChEBI)
Active site552Phosphoserine intermediate

GO annotations

AspectTerm
Molecular Functionadenylylsulfate kinase activity
Molecular FunctionATP binding
Molecular FunctionGTP binding
Molecular FunctionGTPase activity
Molecular Functionsulfate adenylyltransferase (ATP) activity
Biological Processhydrogen sulfide biosynthetic process
Biological Processphosphorylation
Biological Processsulfate assimilation

Keywords

Enzyme and pathway databases

Names & Taxonomy

Protein names

  • Recommended name
    Multifunctional fusion protein

Including 2 domains:

  • Recommended name
    Sulfate adenylyltransferase subunit 1
  • EC number
  • Alternative names
    • ATP-sulfurylase large subunit
    • Sulfate adenylate transferase
      (SAT
      )
  • Recommended name
    Adenylyl-sulfate kinase
  • EC number
  • Alternative names
    • APS kinase
    • ATP adenosine-5'-phosphosulfate 3'-phosphotransferase
    • Adenosine-5'-phosphosulfate kinase

Gene names

    • Name
      cysN
    • Synonyms
      cysC
    • ORF names
      NSU_4047

Organism names

Accessions

  • Primary accession
    G6EI76

Proteomes

PTM/Processing

Keywords

Interaction

Subunit

Heterodimer composed of CysD, the smaller subunit, and CysN.
Sulfate-activating enzymes, NodP and NodQ, may be physically associated.

Protein-protein interaction databases

Family & Domains

Features

Showing features for domain.

TypeIDPosition(s)Description
Domain32-247Tr-type G

Sequence similarities

Belongs to the APS kinase family.
In the C-terminal section; belongs to the APS kinase family.

Phylogenomic databases

Family and domain databases

Sequence

  • Sequence status
    Complete
  • Length
    641
  • Mass (Da)
    70,927
  • Last updated
    2012-01-25 v1
  • Checksum
    52631E2ADA1379B5
MTDTKTHNDPVYVVDELISKDIDAYLEQHEHKTMLRFITCGSVDDGKSTLIGRLLYDSKMIFEDQLDALTSDSKKVGTQGQEIDFALLVDGLAAEREQGITIDVAYRFFNTEKRKFIVADCPGHEQYTRNMVTGASTADLAVILIDARKGVLVQTRRHSYLCHLIGIKNIVLAVNKMDLVDYSQEVFDKIVADYSAFANSIGIESFTAMPISGFKGDNITTLSANTPWYKGPTLVEHLETVEVISSVDADKPLRMPVQWVNRPNLDFRGFSGLIATGAVRPGDKIRVLPSGKTSTVSKVVTFDGEIEEAVAGESVTVCFEDEIDCSRGSVISIADNPPQTADQFESTIVWMADDALVPGRAYWLKLATQTVSVTVHEPKYTVNVNTMEHMAAKTLELNEIGVAEIVTDKQIVFEPYADNRALGGFILVDKMTNATVAAGMINFSLRRSQNVHWQALDIGRKQHAALKNQTPAVLWFTGLSGSGKSTIANMVEKKLHRMNRHTFLLDGDNVRHGLNKDLGFTETDRIENIRRVGEVSKLMADAGLIVITAFISPFQADREMVRSMLPEGEFFEVFIDTPLKVAEARDVKGLYKKARSGELKNFTGIDSPYEAPRSPEIRIDTTKMSPEEAANLIVDTLLGDA

Keywords

Sequence databases

Nucleotide SequenceProtein SequenceMolecule TypeStatus
AGFM01000064
EMBL· GenBank· DDBJ
EHJ58818.1
EMBL· GenBank· DDBJ
Genomic DNA

Genome annotation databases

Similar Proteins

Disclaimer

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