G5EFY7 · PQM1_CAEEL

  • Protein
    Zinc finger transcription factor pqm-1
  • Gene
    pqm-1
  • Status
    UniProtKB reviewed (Swiss-Prot)
  • Amino acids
  • Protein existence
    Evidence at protein level
  • Annotation score
    5/5

Function

function

Zinc finger transcription factor which acts as both a transcriptional activator and repressor (PubMed:23911329, PubMed:30956009).
Binds to the promoters of genes that contain the 5'-CTTATCA-3' DNA consensus sequence in their regulatory region (PubMed:23911329, PubMed:31532389).
Functions downstream of the Insulin/IGF-1-like signaling (IIS) mediated pathway (PubMed:23911329, PubMed:27401555).
Involved in normal development, lifespan, stress response, lipid metabolism, innate immunity and exit from the developmentally arrested larval state known as dauer (PubMed:23911329, PubMed:27401555, PubMed:29949773, PubMed:31532389, PubMed:33009389).
Required for stress-induced expression of hsp-90 and resistance to heat stress, perhaps as part of a systemic stress signaling pathway (PubMed:29949773).
Involved in maintenance of proteostasis (PubMed:29949773).
Under hypoxic stress increases lipid levels by positively regulating fatty acid synthesis via fat-7 expression (PubMed:33009389).
Associates with homeobox protein ceh-60 at the promoters of some stress-responsive genes to regulate expression; may require phosphorylation for transcriptional repression activity (PubMed:30956009).
Acts downstream of nhr-14 to activate transcription of intestinal metal transporter smf-3, modulating innate immunity and iron uptake (PubMed:31532389).
May act downstream of the mTORC2 signaling mediated pathway (PubMed:27401555).
May act in a mutually exclusive manner with the FOXO transcription factor daf-16 (PubMed:23911329, PubMed:27401555).

GO annotations

AspectTerm
Cellular Componentchromosome
Cellular Componentcytoplasm
Cellular Componentnucleus
Molecular Functionchromatin insulator sequence binding
Molecular Functionmetal ion binding
Biological Processcellular lipid metabolic process
Biological Processcellular response to heat
Biological Processdefense response to Gram-negative bacterium
Biological Processdetermination of adult lifespan
Biological Processglycogen metabolic process
Biological Processinnate immune response
Biological Processintracellular oxygen homeostasis
Biological Processnegative regulation of vitellogenesis
Biological Processpositive regulation of dauer entry
Biological Processpositive regulation of DNA-templated transcription
Biological Processpositive regulation of gene expression
Biological Processpositive regulation of vitellogenesis
Biological Processprotein stabilization
Biological Processregulation of gene expression
Biological Processregulation of transcription by RNA polymerase II
Biological Processresponse to hypoxia

Keywords

Names & Taxonomy

Protein names

  • Recommended name
    Zinc finger transcription factor pqm-1
  • Alternative names
    • CePqM132
    • Paraquat responsive protein

Gene names

    • Name
      pqm-1
    • ORF names
      F40F8.7

Organism names

  • Taxonomic identifier
  • Organism
  • Strain
    • Bristol N2
  • Taxonomic lineage
    Eukaryota > Metazoa > Ecdysozoa > Nematoda > Chromadorea > Rhabditida > Rhabditina > Rhabditomorpha > Rhabditoidea > Rhabditidae > Peloderinae > Caenorhabditis

Accessions

  • Primary accession
    G5EFY7

Proteomes

Organism-specific databases

Subcellular Location

Chromosome
Nucleus
Cytoplasm
Note: Nuclear localization under normal conditions, cytoplasmic as a result of heat-stress (PubMed:23911329).
Nuclear localization declines over normal lifespan (PubMed:23911329).
Exposure to Gram-negative bacterium P.aeruginosa enhances nuclear localization (PubMed:31532389).
Hypoxic stress enhances nuclear localization (PubMed:33009389).

Keywords

Phenotypes & Variants

Disruption phenotype

RNAi-mediated knockdown on a daf-2 mutant background shortens lifespan substantially, by up to 45%, but does not alter lifespan on a daf-16 mutant or wild-type background (PubMed:23911329).
Knockdown activates vitellogenesis on either lin-29, rict-1 or sgk-1 mutant backgrounds, but not on daf-2 mutants (PubMed:27401555).
Reduced expression of metal transporter smf-3 on an nhr-14 mutant background (PubMed:27401555).
Enhanced sensitivity to P.aeruginosa infection on an nhr-14 mutant background (PubMed:31532389).

Features

Showing features for mutagenesis.

TypeIDPosition(s)Description
Mutagenesis163In mg477; suppresses defect in vitellogenesis on sgk-1 mutant background.
Mutagenesis266-268Up-regulates expression of stress-response genes which may also be ceh-60 targets.

PTM/Processing

Features

Showing features for chain.

TypeIDPosition(s)Description
ChainPRO_00004527461-295Zinc finger transcription factor pqm-1

Proteomic databases

Expression

Induction

Induced by paraquat, a chemical causing production of reactive oxygen species (ROS).

Developmental stage

Expressed in intestinal cells from larval through to adult stages (PubMed:23911329).
Expressed in neurons during L1 larval stage (PubMed:29949773).

Gene expression databases

Interaction

Subunit

Interacts with ceh-60.

Protein-protein interaction databases

Family & Domains

Features

Showing features for region, zinc finger.

TypeIDPosition(s)Description
Region1-23Disordered
Zinc finger161-183C2H2-type 1; degenerate
Zinc finger227-249C2H2-type 2

Sequence similarities

Keywords

Phylogenomic databases

Family and domain databases

Sequence

  • Sequence status
    Complete
  • Length
    295
  • Mass (Da)
    33,346
  • Last updated
    2011-12-14 v1
  • Checksum
    D504D4DB0687DB15
MSFLNNDFGSPPATSSPPTTMPKLPTIQDMLNNIGASTVNLMQPNPYLMQNQIPLPVPNLPLNPFLHLNPAISQEIIQQFIAMSFNTPNVLASIANMGDDEGPSCNPKMRRGDLLKSVSMDSTEDPPSITLDNNGDMIVPNNDKEGWCRNKKYIEQTENGYMCTVCRKVYGRYNSVSYHVTIYHRNPPIKCNVPNCQFTTREARYIHFHKNYRHGIPLPESIDQGSRKCPHCRHVSKSPAMLEKHIRRHQIKDGLSNINEAIRERTSTICDEAMEIEPAETEVDPIETKPRSCTL

Keywords

Sequence databases

Nucleotide SequenceProtein SequenceMolecule TypeStatus
AF008590
EMBL· GenBank· DDBJ
AAB63299.1
EMBL· GenBank· DDBJ
mRNA
BX284602
EMBL· GenBank· DDBJ
CAA93267.1
EMBL· GenBank· DDBJ
Genomic DNA

Genome annotation databases

Similar Proteins

Disclaimer

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