G5EFY7 · PQM1_CAEEL
- ProteinZinc finger transcription factor pqm-1
- Genepqm-1
- StatusUniProtKB reviewed (Swiss-Prot)
- Organism
- Amino acids295 (go to sequence)
- Protein existenceEvidence at protein level
- Annotation score5/5
Function
function
Zinc finger transcription factor which acts as both a transcriptional activator and repressor (PubMed:23911329, PubMed:30956009).
Binds to the promoters of genes that contain the 5'-CTTATCA-3' DNA consensus sequence in their regulatory region (PubMed:23911329, PubMed:31532389).
Functions downstream of the Insulin/IGF-1-like signaling (IIS) mediated pathway (PubMed:23911329, PubMed:27401555).
Involved in normal development, lifespan, stress response, lipid metabolism, innate immunity and exit from the developmentally arrested larval state known as dauer (PubMed:23911329, PubMed:27401555, PubMed:29949773, PubMed:31532389, PubMed:33009389).
Required for stress-induced expression of hsp-90 and resistance to heat stress, perhaps as part of a systemic stress signaling pathway (PubMed:29949773).
Involved in maintenance of proteostasis (PubMed:29949773).
Under hypoxic stress increases lipid levels by positively regulating fatty acid synthesis via fat-7 expression (PubMed:33009389).
Associates with homeobox protein ceh-60 at the promoters of some stress-responsive genes to regulate expression; may require phosphorylation for transcriptional repression activity (PubMed:30956009).
Acts downstream of nhr-14 to activate transcription of intestinal metal transporter smf-3, modulating innate immunity and iron uptake (PubMed:31532389).
May act downstream of the mTORC2 signaling mediated pathway (PubMed:27401555).
May act in a mutually exclusive manner with the FOXO transcription factor daf-16 (PubMed:23911329, PubMed:27401555).
Binds to the promoters of genes that contain the 5'-CTTATCA-3' DNA consensus sequence in their regulatory region (PubMed:23911329, PubMed:31532389).
Functions downstream of the Insulin/IGF-1-like signaling (IIS) mediated pathway (PubMed:23911329, PubMed:27401555).
Involved in normal development, lifespan, stress response, lipid metabolism, innate immunity and exit from the developmentally arrested larval state known as dauer (PubMed:23911329, PubMed:27401555, PubMed:29949773, PubMed:31532389, PubMed:33009389).
Required for stress-induced expression of hsp-90 and resistance to heat stress, perhaps as part of a systemic stress signaling pathway (PubMed:29949773).
Involved in maintenance of proteostasis (PubMed:29949773).
Under hypoxic stress increases lipid levels by positively regulating fatty acid synthesis via fat-7 expression (PubMed:33009389).
Associates with homeobox protein ceh-60 at the promoters of some stress-responsive genes to regulate expression; may require phosphorylation for transcriptional repression activity (PubMed:30956009).
Acts downstream of nhr-14 to activate transcription of intestinal metal transporter smf-3, modulating innate immunity and iron uptake (PubMed:31532389).
May act downstream of the mTORC2 signaling mediated pathway (PubMed:27401555).
May act in a mutually exclusive manner with the FOXO transcription factor daf-16 (PubMed:23911329, PubMed:27401555).
GO annotations
Aspect | Term | |
---|---|---|
Cellular Component | chromosome | |
Cellular Component | cytoplasm | |
Cellular Component | nucleus | |
Molecular Function | chromatin insulator sequence binding | |
Molecular Function | metal ion binding | |
Biological Process | cellular lipid metabolic process | |
Biological Process | cellular response to heat | |
Biological Process | defense response to Gram-negative bacterium | |
Biological Process | determination of adult lifespan | |
Biological Process | glycogen metabolic process | |
Biological Process | innate immune response | |
Biological Process | intracellular oxygen homeostasis | |
Biological Process | negative regulation of vitellogenesis | |
Biological Process | positive regulation of dauer entry | |
Biological Process | positive regulation of DNA-templated transcription | |
Biological Process | positive regulation of gene expression | |
Biological Process | positive regulation of vitellogenesis | |
Biological Process | protein stabilization | |
Biological Process | regulation of gene expression | |
Biological Process | regulation of transcription by RNA polymerase II | |
Biological Process | response to hypoxia |
Keywords
- Molecular function
- Biological process
- Ligand
Names & Taxonomy
Protein names
- Recommended nameZinc finger transcription factor pqm-1
- Alternative names
Gene names
Organism names
- Organism
- Strain
- Taxonomic lineageEukaryota > Metazoa > Ecdysozoa > Nematoda > Chromadorea > Rhabditida > Rhabditina > Rhabditomorpha > Rhabditoidea > Rhabditidae > Peloderinae > Caenorhabditis
Accessions
- Primary accessionG5EFY7
Proteomes
Organism-specific databases
Subcellular Location
UniProt Annotation
GO Annotation
Note: Nuclear localization under normal conditions, cytoplasmic as a result of heat-stress (PubMed:23911329).
Nuclear localization declines over normal lifespan (PubMed:23911329).
Exposure to Gram-negative bacterium P.aeruginosa enhances nuclear localization (PubMed:31532389).
Hypoxic stress enhances nuclear localization (PubMed:33009389).
Nuclear localization declines over normal lifespan (PubMed:23911329).
Exposure to Gram-negative bacterium P.aeruginosa enhances nuclear localization (PubMed:31532389).
Hypoxic stress enhances nuclear localization (PubMed:33009389).
Keywords
- Cellular component
Phenotypes & Variants
Disruption phenotype
RNAi-mediated knockdown on a daf-2 mutant background shortens lifespan substantially, by up to 45%, but does not alter lifespan on a daf-16 mutant or wild-type background (PubMed:23911329).
Knockdown activates vitellogenesis on either lin-29, rict-1 or sgk-1 mutant backgrounds, but not on daf-2 mutants (PubMed:27401555).
Reduced expression of metal transporter smf-3 on an nhr-14 mutant background (PubMed:27401555).
Enhanced sensitivity to P.aeruginosa infection on an nhr-14 mutant background (PubMed:31532389).
Knockdown activates vitellogenesis on either lin-29, rict-1 or sgk-1 mutant backgrounds, but not on daf-2 mutants (PubMed:27401555).
Reduced expression of metal transporter smf-3 on an nhr-14 mutant background (PubMed:27401555).
Enhanced sensitivity to P.aeruginosa infection on an nhr-14 mutant background (PubMed:31532389).
Features
Showing features for mutagenesis.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Mutagenesis | 163 | In mg477; suppresses defect in vitellogenesis on sgk-1 mutant background. | ||||
Sequence: C → Y | ||||||
Mutagenesis | 266-268 | Up-regulates expression of stress-response genes which may also be ceh-60 targets. | ||||
Sequence: TST → AAA |
PTM/Processing
Features
Showing features for chain.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Chain | PRO_0000452746 | 1-295 | Zinc finger transcription factor pqm-1 | |||
Sequence: MSFLNNDFGSPPATSSPPTTMPKLPTIQDMLNNIGASTVNLMQPNPYLMQNQIPLPVPNLPLNPFLHLNPAISQEIIQQFIAMSFNTPNVLASIANMGDDEGPSCNPKMRRGDLLKSVSMDSTEDPPSITLDNNGDMIVPNNDKEGWCRNKKYIEQTENGYMCTVCRKVYGRYNSVSYHVTIYHRNPPIKCNVPNCQFTTREARYIHFHKNYRHGIPLPESIDQGSRKCPHCRHVSKSPAMLEKHIRRHQIKDGLSNINEAIRERTSTICDEAMEIEPAETEVDPIETKPRSCTL |
Proteomic databases
Structure
Family & Domains
Features
Showing features for region, zinc finger.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Region | 1-23 | Disordered | ||||
Sequence: MSFLNNDFGSPPATSSPPTTMPK | ||||||
Zinc finger | 161-183 | C2H2-type 1; degenerate | ||||
Sequence: YMCTVCRKVYGRYNSVSYHVTIY | ||||||
Zinc finger | 227-249 | C2H2-type 2 | ||||
Sequence: RKCPHCRHVSKSPAMLEKHIRRH |
Sequence similarities
Belongs to the krueppel C2H2-type zinc-finger protein family.
Keywords
- Domain
Phylogenomic databases
Family and domain databases
Sequence
- Sequence statusComplete
- Length295
- Mass (Da)33,346
- Last updated2011-12-14 v1
- ChecksumD504D4DB0687DB15
Keywords
- Technical term
Sequence databases
Nucleotide Sequence | Protein Sequence | Molecule Type | Status | |
---|---|---|---|---|
AF008590 EMBL· GenBank· DDBJ | AAB63299.1 EMBL· GenBank· DDBJ | mRNA | ||
BX284602 EMBL· GenBank· DDBJ | CAA93267.1 EMBL· GenBank· DDBJ | Genomic DNA |