G5EFF4 · SEM4_CAEEL

  • Protein
    Spalt-like protein sem-4
  • Gene
    sem-4
  • Status
    UniProtKB reviewed (Swiss-Prot)
  • Amino acids
  • Protein existence
    Evidence at protein level
  • Annotation score
    5/5

Function

function

Transcription factor, involved in positive and negative modulation of transcription (PubMed:12835398, PubMed:28614700).
Binds to multiple DNA sequence motifs in the regulatory elements of target genes, including homeobox selector egl-5 and LIM homeobox mec-3 (PubMed:12835398).
Involved in cell-fate regulation in multiple lineages, including neuronal, mesodermal and vulval (PubMed:10926783, PubMed:12835398, PubMed:28614700, PubMed:8756352).
Required to regulate the fate of PLM touch receptor neurons, acting via negative modulation of transcription of egl-5 and mec-3 (PubMed:12835398).
May modulate gene expression by interacting with different transcription factors during neuronal and mesodermal cell development (PubMed:8756352).
Promotes the proliferative sex myoblast (SM) fate, in a cell autonomous manner, acting via the SoxC transcription factor sem-2 (PubMed:28614700).
Involved in vulval cell-fate determination, acting by regulating expression of homeobox protein lin-39, and may link lin-39 to incoming signaling pathways (PubMed:10926783).
Plays a role in detoxification of reactive oxygen species (ROS), by regulating expression of transcription factor skn-1 and the phase II detoxification genes (PubMed:32718932).

GO annotations

all annotationsall molecular functionvirus receptor activitydna bindingrna bindingcytoskeletal motor activitycatalytic activitygtpase activitystructural molecule activitytransporter activitycytoskeletal protein bindinglipid bindingcyclase activityantioxidant activityoxidoreductase activitytransferase activityhydrolase activitylyase activityisomerase activityligase activityprotein tag activitycargo receptor activityhistone bindingprotein folding chaperonetranslation regulator activitynutrient reservoir activityreceptor ligand activitymolecular transducer activitymolecular adaptor activitytoxin activitycell adhesion mediator activitymolecular function regulator activityvirus coreceptor activitycatalytic activity, acting on a proteincatalytic activity, acting on dnacatalytic activity, acting on rnamolecular carrier activitytranscription regulator activitygeneral transcription initiation factor activitymolecular sensor activitymolecular sequestering activityatp-dependent activityother molecular functionall biological processmitotic cell cyclecytokinesiscytoplasmic translationimmune system processmuscle system processcirculatory system processrenal system processrespiratory system processcarbohydrate metabolic processgeneration of precursor metabolites and energydna replicationdna repairdna recombinationchromatin organizationdna-templated transcriptionregulation of dna-templated transcriptiontrna metabolic processprotein foldingprotein glycosylationamino acid metabolic processmodified amino acid metabolic processlipid metabolic processvitamin metabolic processsulfur compound metabolic processintracellular protein transportnucleocytoplasmic transportautophagyinflammatory responsemitochondrion organizationcytoskeleton organizationmicrotubule-based movementperoxisome organizationlysosome organizationchromosome segregationcell adhesionestablishment or maintenance of cell polarityprogrammed cell deathphotosynthesismrna metabolic processsnrna metabolic processvesicle-mediated transportreproductive processdigestive system processsignalingcell differentiationprotein catabolic processextracellular matrix organizationregulatory ncrna-mediated gene silencingtelomere organizationcell junction organizationwound healingribosome biogenesiscilium organizationanatomical structure developmentcell motilitynervous system processendocrine processprotein maturationtransmembrane transportnucleobase-containing small molecule metabolic processhepaticobiliary system processmembrane organizationprotein-containing complex assemblycell wall organization or biogenesisnitrogen cycle metabolic processprotein localization to plasma membranedefense response to other organismdetoxificationmeiotic nuclear divisionmitotic nuclear divisionmitochondrial gene expressioncarbohydrate derivative metabolic processother biological processall cellular componentnuclear chromosomeextracellular regionextracellular spacecell wallnucleusnuclear envelopenucleoplasmchromosomenucleolusmitochondrionlysosomeendosomevacuoleperoxisomeendoplasmic reticulumgolgi apparatuslipid dropletmicrotubule organizing centercytosolribosomecytoskeletonplasma membraneciliumplastidthylakoidexternal encapsulating structureextracellular matrixcytoplasmic vesicleorganelleother cellular component
Cell color indicative of number of GO terms
AspectTerm
Cellular Componentnucleus
Molecular FunctionDNA-binding transcription factor activity, RNA polymerase II-specific
Molecular Functionmetal ion binding
Molecular FunctionRNA polymerase II cis-regulatory region sequence-specific DNA binding
Molecular FunctionRNA polymerase II transcription regulatory region sequence-specific DNA binding
Biological Processmesodermal cell differentiation
Biological Processmuscle cell differentiation
Biological Processmyoblast fate specification
Biological Processnegative regulation of transcription by RNA polymerase II
Biological Processneuroblast differentiation
Biological Processpositive regulation of gene expression
Biological Processpositive regulation of transcription by RNA polymerase II
Biological Processregulation of transcription by RNA polymerase II
Biological Processresponse to oxidative stress
Biological Processtransdifferentiation
Biological Processvulval cell fate determination

Keywords

Names & Taxonomy

Protein names

  • Recommended name
    Spalt-like protein sem-4
  • Alternative names
    • Sex muscle abnormal protein sem-4
    • Transcription factor sem-4

Gene names

    • Name
      sem-4
    • ORF names
      F15C11.1

Organism names

  • Taxonomic identifier
  • Organism
  • Strain
    • Bristol N2
  • Taxonomic lineage
    Eukaryota > Metazoa > Ecdysozoa > Nematoda > Chromadorea > Rhabditida > Rhabditina > Rhabditomorpha > Rhabditoidea > Rhabditidae > Peloderinae > Caenorhabditis

Accessions

  • Primary accession
    G5EFF4
  • Secondary accessions
    • Q7JNU7

Proteomes

Organism-specific databases

Subcellular Location

Keywords

Phenotypes & Variants

Features

Showing features for mutagenesis.

TypeIDPosition(s)Description
Mutagenesis275-744In h769; sex myoblasts (SM) are transformed into body-wall muscles (BWM), and coelomocytes transformed into their ventral counterparts, the SM progenitors, which subsequently give rise to two BWMs. Expression of sem-2 is abolished in the M mesoblast-derived lineage.
Mutagenesis323In n2654; severe pleiotropic abnormalities, including defects in the development of the sex myoblasts, a mesodermal cell type, and of two classes of neurons, the DVB and AVLGABAergic motor neurons. Absence of sex myoblasts in 7% of L3 larvae, defects in differentiation of hermaphrodite-specific neurons (HSN), and M mesoblast-derived coelomocytes (CC) absent in 2% of L3 larvae. Distended intestine in 84% of adults. Vulval precursor cells (VPC), P5.p and P7.p, appear to adopt normal cell fates. Reduced touch-response.
Mutagenesis383-744In ku200; disrupts function in the vulva and mesoderm. Vulval precursor cells (VPC), P5.p and P7.p, adopt abnormal cell fates. Expression of lin-39 in vulval cells reduced drastically.
Mutagenesis569-744In n1378; severe pleiotropic abnormalities, including defects in the development of the sex myoblasts, a mesodermal cell type, and of two classes of neurons, the DVB and AVLGABAergic motor neurons. Absence of sex myoblasts in 98% of L3 larvae, defects in differentiation of hermaphrodite-specific neurons (HSN), and M mesoblast-derived coelomocytes (CC) absent in 30% of L3 larvae. Sex myoblasts (SM) are transformed into body-wall muscles (BWM), and coelomocytes transformed into their ventral counterparts, the SM progenitors, which subsequently give rise to two BWMs. Distended intestine in only 2% of adults. Vulval precursor cells (VPC), P5.p and P7.p, adopt abnormal cell fates. Reduced touch-response. Reduced gst-4 expression.

PTM/Processing

Features

Showing features for chain.

TypeIDPosition(s)Description
ChainPRO_00004545901-744Spalt-like protein sem-4

Proteomic databases

Expression

Developmental stage

Expressed throughout the mesodermal (M) lineage from the 1-M stage to the 18-M stage, and in the sex myoblast (SM) sub-lineage from the 2-SM stage to the 16-SM stage, before the cells differentiate (PubMed:28614700).
Expression declines significantly in the differentiated coelomocytes (CC) and vulval muscles, but remains in the differentiated body-wall muscles (BWM) (PubMed:28614700).
Expressed in the vulval precursor cells (VPC) during larval development (PubMed:10926783).

Gene expression databases

    • WBGene00004773Expressed in pharyngeal muscle cell (C elegans) and 3 other cell types or tissues

Interaction

Protein-protein interaction databases

Family & Domains

Features

Showing features for region, zinc finger, compositional bias.

TypeIDPosition(s)Description
Region6-32Disordered
Zinc finger99-124C2H2-type 1
Zinc finger305-327C2H2-type 2
Zinc finger333-355C2H2-type 3
Zinc finger411-433C2H2-type 4
Region487-530Disordered
Compositional bias511-530Polar residues
Region542-562Disordered
Zinc finger589-611C2H2-type 5
Zinc finger617-639C2H2-type 6
Zinc finger701-723C2H2-type 7
Region725-744Disordered

Sequence similarities

Keywords

Phylogenomic databases

Family and domain databases

Sequence

  • Sequence status
    Complete
  • Length
    744
  • Mass (Da)
    81,694
  • Last updated
    2011-12-14 v1
  • Checksum
    BCF468C639A30182
MNELLAEMAAVSSRRKQSKPRRMSGEGDAMMSPIDLSTKSFDENNCEKGAGGALPLEDRSNILPHFSVPFANPQQFLSLCAQLGNSSSRNVSSTASTTSSCPIQSCSQSFSSPAALTWHVLDAHEDEQEIFSCDVCTTTFSNGQDIREHKCQKTLASRSTSVPPSTIPSSVCFLSTPTTPCLQFSINESIGTSEIREEDEEEDMDVEDGEHVANQLFGHLLQKSDDKSKMASLFNHAFPPFAAFPNMPPPFLMRQPFDPRADVFAAGRHDNDDDWEALMEISTSDEAEKIRALVGDKAVPTTDPNQCILCRRVLSCKSALQMHYRTHTGERPFKCKICQRAFTTKGNLKTHMGVHRSKHSFRGLPISLPPQLAAMHQHQHQIAPPQRIHIHNPPTSAASAAAAVAQIQASQQCPICQQRFLNAGELAVHITEHRNSLTQPPRVMPTPTTTRVQTFPFVPFFTTPPSLNATDMSTQFNLANILSAQLKNDSSPNTDTSSVEEKITRDDPPKMASLSPSNSSDSSSSVRQDILESSEFEEKLKKLEEPPILEQQVSTTPNPKNENPLLAMQKMWAETEPPPPRQMPVLSKHQCGVCFKHFSSSSALQIHMRTHTGDKPFKCDMCGRAFTTRGNLKVHMGTHSWQQSPSRRGRRIFDVASSVTEKPMLQSPILPTSGAPGASPLAMLGPNGLSGLEMMMMLWRTVCSVCQKVCQSPNELEQHLKEHLNNGSSAAPTPLASAATPPPS

Sequence caution

The sequence AAB03334.1 differs from that shown. Reason: Erroneous initiation Truncated N-terminus.

Features

Showing features for compositional bias.

TypeIDPosition(s)Description
Compositional bias511-530Polar residues

Keywords

Sequence databases

Nucleotide SequenceProtein SequenceMolecule TypeStatus
U60112
EMBL· GenBank· DDBJ
AAB03333.1
EMBL· GenBank· DDBJ
mRNA
U60113
EMBL· GenBank· DDBJ
AAB03334.1
EMBL· GenBank· DDBJ
mRNA Different initiation
BX284601
EMBL· GenBank· DDBJ
CAA95798.1
EMBL· GenBank· DDBJ
Genomic DNA

Genome annotation databases

Similar Proteins

Disclaimer

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