G5EFF4 · SEM4_CAEEL
- ProteinSpalt-like protein sem-4
- Genesem-4
- StatusUniProtKB reviewed (Swiss-Prot)
- Organism
- Amino acids744 (go to sequence)
- Protein existenceEvidence at protein level
- Annotation score5/5
Function
function
Transcription factor, involved in positive and negative modulation of transcription (PubMed:12835398, PubMed:28614700).
Binds to multiple DNA sequence motifs in the regulatory elements of target genes, including homeobox selector egl-5 and LIM homeobox mec-3 (PubMed:12835398).
Involved in cell-fate regulation in multiple lineages, including neuronal, mesodermal and vulval (PubMed:10926783, PubMed:12835398, PubMed:28614700, PubMed:8756352).
Required to regulate the fate of PLM touch receptor neurons, acting via negative modulation of transcription of egl-5 and mec-3 (PubMed:12835398).
May modulate gene expression by interacting with different transcription factors during neuronal and mesodermal cell development (PubMed:8756352).
Promotes the proliferative sex myoblast (SM) fate, in a cell autonomous manner, acting via the SoxC transcription factor sem-2 (PubMed:28614700).
Involved in vulval cell-fate determination, acting by regulating expression of homeobox protein lin-39, and may link lin-39 to incoming signaling pathways (PubMed:10926783).
Plays a role in detoxification of reactive oxygen species (ROS), by regulating expression of transcription factor skn-1 and the phase II detoxification genes (PubMed:32718932).
Binds to multiple DNA sequence motifs in the regulatory elements of target genes, including homeobox selector egl-5 and LIM homeobox mec-3 (PubMed:12835398).
Involved in cell-fate regulation in multiple lineages, including neuronal, mesodermal and vulval (PubMed:10926783, PubMed:12835398, PubMed:28614700, PubMed:8756352).
Required to regulate the fate of PLM touch receptor neurons, acting via negative modulation of transcription of egl-5 and mec-3 (PubMed:12835398).
May modulate gene expression by interacting with different transcription factors during neuronal and mesodermal cell development (PubMed:8756352).
Promotes the proliferative sex myoblast (SM) fate, in a cell autonomous manner, acting via the SoxC transcription factor sem-2 (PubMed:28614700).
Involved in vulval cell-fate determination, acting by regulating expression of homeobox protein lin-39, and may link lin-39 to incoming signaling pathways (PubMed:10926783).
Plays a role in detoxification of reactive oxygen species (ROS), by regulating expression of transcription factor skn-1 and the phase II detoxification genes (PubMed:32718932).
GO annotations
all annotations | all molecular function | virus receptor activity | dna binding | rna binding | cytoskeletal motor activity | catalytic activity | gtpase activity | structural molecule activity | transporter activity | cytoskeletal protein binding | lipid binding | cyclase activity | antioxidant activity | oxidoreductase activity | transferase activity | hydrolase activity | lyase activity | isomerase activity | ligase activity | protein tag activity | cargo receptor activity | histone binding | protein folding chaperone | translation regulator activity | nutrient reservoir activity | receptor ligand activity | molecular transducer activity | molecular adaptor activity | toxin activity | cell adhesion mediator activity | molecular function regulator activity | virus coreceptor activity | catalytic activity, acting on a protein | catalytic activity, acting on dna | catalytic activity, acting on rna | molecular carrier activity | transcription regulator activity | general transcription initiation factor activity | molecular sensor activity | molecular sequestering activity | atp-dependent activity | other molecular function | all biological process | mitotic cell cycle | cytokinesis | cytoplasmic translation | immune system process | muscle system process | circulatory system process | renal system process | respiratory system process | carbohydrate metabolic process | generation of precursor metabolites and energy | dna replication | dna repair | dna recombination | chromatin organization | dna-templated transcription | regulation of dna-templated transcription | trna metabolic process | protein folding | protein glycosylation | amino acid metabolic process | modified amino acid metabolic process | lipid metabolic process | vitamin metabolic process | sulfur compound metabolic process | intracellular protein transport | nucleocytoplasmic transport | autophagy | inflammatory response | mitochondrion organization | cytoskeleton organization | microtubule-based movement | peroxisome organization | lysosome organization | chromosome segregation | cell adhesion | establishment or maintenance of cell polarity | programmed cell death | photosynthesis | mrna metabolic process | snrna metabolic process | vesicle-mediated transport | reproductive process | digestive system process | signaling | cell differentiation | protein catabolic process | extracellular matrix organization | regulatory ncrna-mediated gene silencing | telomere organization | cell junction organization | wound healing | ribosome biogenesis | cilium organization | anatomical structure development | cell motility | nervous system process | endocrine process | protein maturation | transmembrane transport | nucleobase-containing small molecule metabolic process | hepaticobiliary system process | membrane organization | protein-containing complex assembly | cell wall organization or biogenesis | nitrogen cycle metabolic process | protein localization to plasma membrane | defense response to other organism | detoxification | meiotic nuclear division | mitotic nuclear division | mitochondrial gene expression | carbohydrate derivative metabolic process | other biological process | all cellular component | nuclear chromosome | extracellular region | extracellular space | cell wall | nucleus | nuclear envelope | nucleoplasm | chromosome | nucleolus | mitochondrion | lysosome | endosome | vacuole | peroxisome | endoplasmic reticulum | golgi apparatus | lipid droplet | microtubule organizing center | cytosol | ribosome | cytoskeleton | plasma membrane | cilium | plastid | thylakoid | external encapsulating structure | extracellular matrix | cytoplasmic vesicle | organelle | other cellular component | |||
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Aspect | Term | |
---|---|---|
Cellular Component | nucleus | |
Molecular Function | DNA-binding transcription factor activity, RNA polymerase II-specific | |
Molecular Function | metal ion binding | |
Molecular Function | RNA polymerase II cis-regulatory region sequence-specific DNA binding | |
Molecular Function | RNA polymerase II transcription regulatory region sequence-specific DNA binding | |
Biological Process | mesodermal cell differentiation | |
Biological Process | muscle cell differentiation | |
Biological Process | myoblast fate specification | |
Biological Process | negative regulation of transcription by RNA polymerase II | |
Biological Process | neuroblast differentiation | |
Biological Process | positive regulation of gene expression | |
Biological Process | positive regulation of transcription by RNA polymerase II | |
Biological Process | regulation of transcription by RNA polymerase II | |
Biological Process | response to oxidative stress | |
Biological Process | transdifferentiation | |
Biological Process | vulval cell fate determination |
Keywords
- Biological process
- Ligand
Names & Taxonomy
Protein names
- Recommended nameSpalt-like protein sem-4
- Alternative names
Gene names
Organism names
- Organism
- Strain
- Taxonomic lineageEukaryota > Metazoa > Ecdysozoa > Nematoda > Chromadorea > Rhabditida > Rhabditina > Rhabditomorpha > Rhabditoidea > Rhabditidae > Peloderinae > Caenorhabditis
Accessions
- Primary accessionG5EFF4
- Secondary accessions
Proteomes
Organism-specific databases
Subcellular Location
Phenotypes & Variants
Features
Showing features for mutagenesis.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Mutagenesis | 275-744 | In h769; sex myoblasts (SM) are transformed into body-wall muscles (BWM), and coelomocytes transformed into their ventral counterparts, the SM progenitors, which subsequently give rise to two BWMs. Expression of sem-2 is abolished in the M mesoblast-derived lineage. | ||||
Sequence: Missing | ||||||
Mutagenesis | 323 | In n2654; severe pleiotropic abnormalities, including defects in the development of the sex myoblasts, a mesodermal cell type, and of two classes of neurons, the DVB and AVLGABAergic motor neurons. Absence of sex myoblasts in 7% of L3 larvae, defects in differentiation of hermaphrodite-specific neurons (HSN), and M mesoblast-derived coelomocytes (CC) absent in 2% of L3 larvae. Distended intestine in 84% of adults. Vulval precursor cells (VPC), P5.p and P7.p, appear to adopt normal cell fates. Reduced touch-response. | ||||
Sequence: H → Y | ||||||
Mutagenesis | 383-744 | In ku200; disrupts function in the vulva and mesoderm. Vulval precursor cells (VPC), P5.p and P7.p, adopt abnormal cell fates. Expression of lin-39 in vulval cells reduced drastically. | ||||
Sequence: Missing | ||||||
Mutagenesis | 569-744 | In n1378; severe pleiotropic abnormalities, including defects in the development of the sex myoblasts, a mesodermal cell type, and of two classes of neurons, the DVB and AVLGABAergic motor neurons. Absence of sex myoblasts in 98% of L3 larvae, defects in differentiation of hermaphrodite-specific neurons (HSN), and M mesoblast-derived coelomocytes (CC) absent in 30% of L3 larvae. Sex myoblasts (SM) are transformed into body-wall muscles (BWM), and coelomocytes transformed into their ventral counterparts, the SM progenitors, which subsequently give rise to two BWMs. Distended intestine in only 2% of adults. Vulval precursor cells (VPC), P5.p and P7.p, adopt abnormal cell fates. Reduced touch-response. Reduced gst-4 expression. | ||||
Sequence: Missing |
PTM/Processing
Features
Showing features for chain.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Chain | PRO_0000454590 | 1-744 | Spalt-like protein sem-4 | |||
Sequence: MNELLAEMAAVSSRRKQSKPRRMSGEGDAMMSPIDLSTKSFDENNCEKGAGGALPLEDRSNILPHFSVPFANPQQFLSLCAQLGNSSSRNVSSTASTTSSCPIQSCSQSFSSPAALTWHVLDAHEDEQEIFSCDVCTTTFSNGQDIREHKCQKTLASRSTSVPPSTIPSSVCFLSTPTTPCLQFSINESIGTSEIREEDEEEDMDVEDGEHVANQLFGHLLQKSDDKSKMASLFNHAFPPFAAFPNMPPPFLMRQPFDPRADVFAAGRHDNDDDWEALMEISTSDEAEKIRALVGDKAVPTTDPNQCILCRRVLSCKSALQMHYRTHTGERPFKCKICQRAFTTKGNLKTHMGVHRSKHSFRGLPISLPPQLAAMHQHQHQIAPPQRIHIHNPPTSAASAAAAVAQIQASQQCPICQQRFLNAGELAVHITEHRNSLTQPPRVMPTPTTTRVQTFPFVPFFTTPPSLNATDMSTQFNLANILSAQLKNDSSPNTDTSSVEEKITRDDPPKMASLSPSNSSDSSSSVRQDILESSEFEEKLKKLEEPPILEQQVSTTPNPKNENPLLAMQKMWAETEPPPPRQMPVLSKHQCGVCFKHFSSSSALQIHMRTHTGDKPFKCDMCGRAFTTRGNLKVHMGTHSWQQSPSRRGRRIFDVASSVTEKPMLQSPILPTSGAPGASPLAMLGPNGLSGLEMMMMLWRTVCSVCQKVCQSPNELEQHLKEHLNNGSSAAPTPLASAATPPPS |
Proteomic databases
Expression
Developmental stage
Expressed throughout the mesodermal (M) lineage from the 1-M stage to the 18-M stage, and in the sex myoblast (SM) sub-lineage from the 2-SM stage to the 16-SM stage, before the cells differentiate (PubMed:28614700).
Expression declines significantly in the differentiated coelomocytes (CC) and vulval muscles, but remains in the differentiated body-wall muscles (BWM) (PubMed:28614700).
Expressed in the vulval precursor cells (VPC) during larval development (PubMed:10926783).
Expression declines significantly in the differentiated coelomocytes (CC) and vulval muscles, but remains in the differentiated body-wall muscles (BWM) (PubMed:28614700).
Expressed in the vulval precursor cells (VPC) during larval development (PubMed:10926783).
Gene expression databases
Structure
Family & Domains
Features
Showing features for region, zinc finger, compositional bias.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Region | 6-32 | Disordered | ||||
Sequence: AEMAAVSSRRKQSKPRRMSGEGDAMMS | ||||||
Zinc finger | 99-124 | C2H2-type 1 | ||||
Sequence: SSCPIQSCSQSFSSPAALTWHVLDAH | ||||||
Zinc finger | 305-327 | C2H2-type 2 | ||||
Sequence: NQCILCRRVLSCKSALQMHYRTH | ||||||
Zinc finger | 333-355 | C2H2-type 3 | ||||
Sequence: FKCKICQRAFTTKGNLKTHMGVH | ||||||
Zinc finger | 411-433 | C2H2-type 4 | ||||
Sequence: QQCPICQQRFLNAGELAVHITEH | ||||||
Region | 487-530 | Disordered | ||||
Sequence: KNDSSPNTDTSSVEEKITRDDPPKMASLSPSNSSDSSSSVRQDI | ||||||
Compositional bias | 511-530 | Polar residues | ||||
Sequence: MASLSPSNSSDSSSSVRQDI | ||||||
Region | 542-562 | Disordered | ||||
Sequence: KLEEPPILEQQVSTTPNPKNE | ||||||
Zinc finger | 589-611 | C2H2-type 5 | ||||
Sequence: HQCGVCFKHFSSSSALQIHMRTH | ||||||
Zinc finger | 617-639 | C2H2-type 6 | ||||
Sequence: FKCDMCGRAFTTRGNLKVHMGTH | ||||||
Zinc finger | 701-723 | C2H2-type 7 | ||||
Sequence: TVCSVCQKVCQSPNELEQHLKEH | ||||||
Region | 725-744 | Disordered | ||||
Sequence: NNGSSAAPTPLASAATPPPS |
Sequence similarities
Belongs to the sal C2H2-type zinc-finger protein family.
Keywords
- Domain
Phylogenomic databases
Family and domain databases
Sequence
- Sequence statusComplete
- Length744
- Mass (Da)81,694
- Last updated2011-12-14 v1
- ChecksumBCF468C639A30182
Sequence caution
Features
Showing features for compositional bias.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Compositional bias | 511-530 | Polar residues | ||||
Sequence: MASLSPSNSSDSSSSVRQDI |
Keywords
- Technical term
Sequence databases
Nucleotide Sequence | Protein Sequence | Molecule Type | Status | |
---|---|---|---|---|
U60112 EMBL· GenBank· DDBJ | AAB03333.1 EMBL· GenBank· DDBJ | mRNA | ||
U60113 EMBL· GenBank· DDBJ | AAB03334.1 EMBL· GenBank· DDBJ | mRNA | Different initiation | |
BX284601 EMBL· GenBank· DDBJ | CAA95798.1 EMBL· GenBank· DDBJ | Genomic DNA |