G5EEM5 · ZYG9_CAEEL
- ProteinZygote defective protein 9
- Genezyg-9
- StatusUniProtKB reviewed (Swiss-Prot)
- Organism
- Amino acids1415 (go to sequence)
- Protein existenceEvidence at protein level
- Annotation score5/5
Function
function
Plays a major role in organizing microtubules and spindle poles during mitosis and meiosis in one-cell stage embryos (PubMed:16971515).
Required for default nucleus positioning in oocytes (PubMed:16971515).
Required for default nucleus positioning in oocytes (PubMed:16971515).
GO annotations
all annotations | all molecular function | virus receptor activity | dna binding | rna binding | cytoskeletal motor activity | catalytic activity | gtpase activity | structural molecule activity | transporter activity | cytoskeletal protein binding | lipid binding | cyclase activity | antioxidant activity | oxidoreductase activity | transferase activity | hydrolase activity | lyase activity | isomerase activity | ligase activity | protein tag activity | cargo receptor activity | histone binding | protein folding chaperone | translation regulator activity | nutrient reservoir activity | receptor ligand activity | molecular transducer activity | molecular adaptor activity | toxin activity | cell adhesion mediator activity | molecular function regulator activity | virus coreceptor activity | catalytic activity, acting on a protein | catalytic activity, acting on dna | catalytic activity, acting on rna | molecular carrier activity | transcription regulator activity | general transcription initiation factor activity | molecular sensor activity | molecular sequestering activity | atp-dependent activity | other molecular function | all biological process | mitotic cell cycle | cytokinesis | cytoplasmic translation | immune system process | muscle system process | circulatory system process | renal system process | respiratory system process | carbohydrate metabolic process | generation of precursor metabolites and energy | dna replication | dna repair | dna recombination | chromatin organization | dna-templated transcription | regulation of dna-templated transcription | trna metabolic process | protein folding | protein glycosylation | amino acid metabolic process | modified amino acid metabolic process | lipid metabolic process | vitamin metabolic process | sulfur compound metabolic process | intracellular protein transport | nucleocytoplasmic transport | autophagy | inflammatory response | mitochondrion organization | cytoskeleton organization | microtubule-based movement | peroxisome organization | lysosome organization | chromosome segregation | cell adhesion | establishment or maintenance of cell polarity | programmed cell death | photosynthesis | mrna metabolic process | snrna metabolic process | vesicle-mediated transport | reproductive process | digestive system process | signaling | cell differentiation | protein catabolic process | extracellular matrix organization | regulatory ncrna-mediated gene silencing | telomere organization | cell junction organization | wound healing | ribosome biogenesis | cilium organization | anatomical structure development | cell motility | nervous system process | endocrine process | protein maturation | transmembrane transport | nucleobase-containing small molecule metabolic process | hepaticobiliary system process | membrane organization | protein-containing complex assembly | cell wall organization or biogenesis | nitrogen cycle metabolic process | protein localization to plasma membrane | defense response to other organism | detoxification | meiotic nuclear division | mitotic nuclear division | mitochondrial gene expression | carbohydrate derivative metabolic process | other biological process | all cellular component | nuclear chromosome | extracellular region | extracellular space | cell wall | nucleus | nuclear envelope | nucleoplasm | chromosome | nucleolus | mitochondrion | lysosome | endosome | vacuole | peroxisome | endoplasmic reticulum | golgi apparatus | lipid droplet | microtubule organizing center | cytosol | ribosome | cytoskeleton | plasma membrane | cilium | plastid | thylakoid | external encapsulating structure | extracellular matrix | cytoplasmic vesicle | organelle | other cellular component | |||
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Aspect | Term | |
---|---|---|
Cellular Component | centrosome | |
Cellular Component | cytoplasm | |
Cellular Component | kinetochore | |
Cellular Component | meiotic spindle | |
Cellular Component | mitotic spindle astral microtubule | |
Cellular Component | mitotic spindle microtubule | |
Cellular Component | spindle pole | |
Molecular Function | microtubule binding | |
Molecular Function | microtubule plus end polymerase | |
Molecular Function | microtubule plus-end binding | |
Biological Process | cell division | |
Biological Process | centrosome duplication | |
Biological Process | embryo development ending in birth or egg hatching | |
Biological Process | establishment or maintenance of microtubule cytoskeleton polarity | |
Biological Process | meiotic spindle organization | |
Biological Process | microtubule polymerization | |
Biological Process | microtubule-based process | |
Biological Process | mitotic spindle organization | |
Biological Process | spindle elongation | |
Biological Process | spindle organization |
Keywords
- Biological process
Names & Taxonomy
Protein names
- Recommended nameZygote defective protein 9
Gene names
Organism names
- Organism
- Strain
- Taxonomic lineageEukaryota > Metazoa > Ecdysozoa > Nematoda > Chromadorea > Rhabditida > Rhabditina > Rhabditomorpha > Rhabditoidea > Rhabditidae > Peloderinae > Caenorhabditis
Accessions
- Primary accessionG5EEM5
Proteomes
Organism-specific databases
Subcellular Location
UniProt Annotation
GO Annotation
Note: Cycles between cytoplasm and centrosome during mitosis in a cell cycle- dependent manner. Centrosomal recruitment requires tbg-1.
Keywords
- Cellular component
Phenotypes & Variants
Disruption phenotype
Maternal-effect embryonic lethal with meiotic and mitotic spindle positioning defects in one-cell stage embryos. RNAi-mediated knockdown results in meiosis and mitosis defects in embryos whereby embryos have one or both polar bodies absent, more than one female pronucleus and display irregular female pronuclear migration, which leads to delayed association with the male pronucleus (PubMed:16971515).
RNAi-mediated knockdown results in defective mitotic spindle positioning and rotation in which spindles are established in the posterior side of the embryo and on the wrong axis (PubMed:16971515).
RNAi-mediated knockdown results in a decrease in length of astral microtubules in embryos, with fewer microtubules extending from their centrosomes during cold conditions (PubMed:16971515).
RNAi-mediated knockdown in a let-711 heteroallelic s2587 and it150 mutant background results in inviable embryos which do not hatch, however the astral microtubule length, polar body, female pronuclear migration and mitotic spindle defects of the single zyg-9 RNAi mutant are partially suppressed (PubMed:16971515).
RNAi-mediated knockdown results in defective mitotic spindle positioning and rotation in which spindles are established in the posterior side of the embryo and on the wrong axis (PubMed:16971515).
RNAi-mediated knockdown results in a decrease in length of astral microtubules in embryos, with fewer microtubules extending from their centrosomes during cold conditions (PubMed:16971515).
RNAi-mediated knockdown in a let-711 heteroallelic s2587 and it150 mutant background results in inviable embryos which do not hatch, however the astral microtubule length, polar body, female pronuclear migration and mitotic spindle defects of the single zyg-9 RNAi mutant are partially suppressed (PubMed:16971515).
Features
Showing features for mutagenesis.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Mutagenesis | 203 | In or628; decrease in abundance at centromeres. | ||||
Sequence: G → E | ||||||
Mutagenesis | 488 | In or593; decrease in abundance at centromeres. | ||||
Sequence: G → E | ||||||
Mutagenesis | 862 | In or635; reduction in tac-1 binding. | ||||
Sequence: I → K | ||||||
Mutagenesis | 1317 | In or643; decrease in abundance at centromeres and in the cytoplasm and reduction in tac-1 binding. | ||||
Sequence: E → K |
PTM/Processing
Features
Showing features for chain.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Chain | PRO_0000416050 | 1-1415 | Zygote defective protein 9 | |||
Sequence: MSNWDYLDEVDILPKLPPNFDELRESKKWQERKEALEALLKVLTDNERLSTKASYAELIGHLQMVLAKDANINCQALAAKCIGKFATGLRAKFSSFAGPLLPVIFEKMKEKKPMLREPLVDCSNEVGRTMQSLETGQEDILAALAKPNPQIKQQTALFVARQLDLVVPAKQPKGFIKAVVPVFGKLTGDADQDVREASLQGLGAVQRIIGDKNVKNLLGDASSDEGKMKKIGEYAEKSTASFAEEQAKNAPPVAPTSSTPSASAASGDPSGGTATAVVSSGAPVAEADPWDFLDAFDVLSKMPDGFDTNIESKKWQERKEALEGLLQLITANPKLDPKANYGALVERLQKVLEKDANINVAALAANCITGIANGLRTKFQPFAVSVTPIIFEKFKEKKPTLRDPLVACIDAVVATTNLEAVGEIVLAALGKPNPSIKTQTDLFLQRCFMKLNSQTMPKKTLKTLIPSLIKHSGDSDSEVREASYAAMGAMMRAIGEKPSLQLLADIASDNLKMSKIKEFHQKALDEAGPAEIAEMVKSIHKADAPPAAAPPKKTAPPKKQPEDEEVVEEEDEPLKPPPGDKKKKVPVKENEENEPPVVAPKAELLLSDNEDKKQRIKEEKQLKLVKWNFQAPTDEHISQLQTLLGNQAKVSLMSQLFHKDFKQHLAALDSLVRLADTSPRSLLSNSDLLLKWCTLRFFETNPAALIKVLELCKVIVELIRDTETPMSQEEVSAFVPYLLLKTGEAKDNMRTSVRDIVNVLSDVVGPLKMTPMLLDALKSKNARQRSECLLVIEYYITNAGISPLKSLSVEKTVAPFVGDKDVNVRNAAINVLVACFKFEGDQMWKAAGRMADKDKSLVEERIKRTGVKPGSGVVTSPPTGGPKILVPQQQGSVVRRPASRSRTREPEPEEVQSDTFTIRQDTMPPKTSSRYALRDDVFSSAMGRLDGTQVITPPQPVNGWSNNTFQMKRTNSSSSISSIDTSDQIQRSINNISSSLADVAQDAMFQVTYVLNQPEQRHLVDRRADLVFRASAAQLDLVIEEFNAGRDVSGTMDACTQMLFILMGGVETEHGLEPLNASPDTVKAIISSVLRCIIQIGNTESGYGMARSLNRLAMRLIYRVELSNLLCGLILAMTESLQMNTGITELVSKLSSKWCDELEKRRAQLRASDIVDSFNAFYVCALTELKMDISDSHILIVDNYLERVILQQGDVVLDAARRLSRPHMHLTSMINKILQMMRERKIDPIMPGTLEARMPQEDEAVVVRSGVQVSIDNILRDTSMAVKHIEQLNILIASSDRSWNEYMEYLKNNPMGELIKELVGECSRKKRIDFNLSHVVKSSMAVFKAMAATGPVQEEGRITPTDINRMDTMIVGTPLSRGDATITRARGNMIRPKRTTLSRDQMANIRHTLDRVKNH |
Proteomic databases
Expression
Developmental stage
Expressed maternally during oogenesis. Observed during mitotic cell cycle from interphase through to early anaphase.
Gene expression databases
Interaction
Subunit
Interacts with tac-1 to form a heterodimer.
Binary interactions
Type | Entry 1 | Entry 2 | Number of experiments | Intact | |
---|---|---|---|---|---|
BINARY | G5EEM5 | tac-1 G5ECG0 | 13 | EBI-320102, EBI-320612 |
Protein-protein interaction databases
Structure
Family & Domains
Features
Showing features for region, coiled coil, repeat, compositional bias.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Region | 1-250 | TOG 1 | ||||
Sequence: MSNWDYLDEVDILPKLPPNFDELRESKKWQERKEALEALLKVLTDNERLSTKASYAELIGHLQMVLAKDANINCQALAAKCIGKFATGLRAKFSSFAGPLLPVIFEKMKEKKPMLREPLVDCSNEVGRTMQSLETGQEDILAALAKPNPQIKQQTALFVARQLDLVVPAKQPKGFIKAVVPVFGKLTGDADQDVREASLQGLGAVQRIIGDKNVKNLLGDASSDEGKMKKIGEYAEKSTASFAEEQAKNA | ||||||
Coiled coil | 21-48 | |||||
Sequence: DELRESKKWQERKEALEALLKVLTDNER | ||||||
Repeat | 30-68 | HEAT 1 | ||||
Sequence: QERKEALEALLKVLTDNERLSTKASYAELIGHLQMVLAK | ||||||
Repeat | 95-132 | HEAT 2 | ||||
Sequence: SFAGPLLPVIFEKMKEKKPMLREPLVDCSNEVGRTMQS | ||||||
Repeat | 135-172 | HEAT 3 | ||||
Sequence: TGQEDILAALAKPNPQIKQQTALFVARQLDLVVPAKQP | ||||||
Repeat | 179-217 | HEAT 4 | ||||
Sequence: VVPVFGKLTGDADQDVREASLQGLGAVQRIIGDKNVKNL | ||||||
Region | 243-278 | Disordered | ||||
Sequence: AEEQAKNAPPVAPTSSTPSASAASGDPSGGTATAVV | ||||||
Compositional bias | 251-277 | Polar residues | ||||
Sequence: PPVAPTSSTPSASAASGDPSGGTATAV | ||||||
Region | 251-530 | TOG 2 | ||||
Sequence: PPVAPTSSTPSASAASGDPSGGTATAVVSSGAPVAEADPWDFLDAFDVLSKMPDGFDTNIESKKWQERKEALEGLLQLITANPKLDPKANYGALVERLQKVLEKDANINVAALAANCITGIANGLRTKFQPFAVSVTPIIFEKFKEKKPTLRDPLVACIDAVVATTNLEAVGEIVLAALGKPNPSIKTQTDLFLQRCFMKLNSQTMPKKTLKTLIPSLIKHSGDSDSEVREASYAAMGAMMRAIGEKPSLQLLADIASDNLKMSKIKEFHQKALDEAGPA | ||||||
Repeat | 339-377 | HEAT 5 | ||||
Sequence: ANYGALVERLQKVLEKDANINVAALAANCITGIANGLRT | ||||||
Repeat | 381-418 | HEAT 6 | ||||
Sequence: PFAVSVTPIIFEKFKEKKPTLRDPLVACIDAVVATTNL | ||||||
Repeat | 420-457 | HEAT 7 | ||||
Sequence: AVGEIVLAALGKPNPSIKTQTDLFLQRCFMKLNSQTMP | ||||||
Repeat | 464-502 | HEAT 8 | ||||
Sequence: LIPSLIKHSGDSDSEVREASYAAMGAMMRAIGEKPSLQL | ||||||
Region | 544-603 | Disordered | ||||
Sequence: APPAAAPPKKTAPPKKQPEDEEVVEEEDEPLKPPPGDKKKKVPVKENEENEPPVVAPKAE | ||||||
Compositional bias | 572-596 | Basic and acidic residues | ||||
Sequence: EPLKPPPGDKKKKVPVKENEENEPP | ||||||
Region | 602-867 | TOG 3 | ||||
Sequence: AELLLSDNEDKKQRIKEEKQLKLVKWNFQAPTDEHISQLQTLLGNQAKVSLMSQLFHKDFKQHLAALDSLVRLADTSPRSLLSNSDLLLKWCTLRFFETNPAALIKVLELCKVIVELIRDTETPMSQEEVSAFVPYLLLKTGEAKDNMRTSVRDIVNVLSDVVGPLKMTPMLLDALKSKNARQRSECLLVIEYYITNAGISPLKSLSVEKTVAPFVGDKDVNVRNAAINVLVACFKFEGDQMWKAAGRMADKDKSLVEERIKRTGV | ||||||
Repeat | 706-743 | HEAT 9 | ||||
Sequence: IKVLELCKVIVELIRDTETPMSQEEVSAFVPYLLLKTG | ||||||
Repeat | 764-801 | HEAT 10 | ||||
Sequence: VGPLKMTPMLLDALKSKNARQRSECLLVIEYYITNAGI | ||||||
Repeat | 804-841 | HEAT 11 | ||||
Sequence: LKSLSVEKTVAPFVGDKDVNVRNAAINVLVACFKFEGD | ||||||
Region | 867-914 | Disordered | ||||
Sequence: VKPGSGVVTSPPTGGPKILVPQQQGSVVRRPASRSRTREPEPEEVQSD | ||||||
Compositional bias | 882-897 | Polar residues | ||||
Sequence: PKILVPQQQGSVVRRP | ||||||
Compositional bias | 898-914 | Basic and acidic residues | ||||
Sequence: ASRSRTREPEPEEVQSD |
Domain
The TOG (tumor overexpressed gene) domains are arranged in the N-terminal region with each domain composed of six (for the most part non-canonical) HEAT repeats and forming a oblong paddle-like structure. 3D-structure analysis shows the presence of additional HEAT repeats that are not detected by sequence-based prediction programs. Intra-HEAT loops are positioned along a face of the TOG domain and bind to a single alpha/beta-tubulin heterodimer. The TOG domains seem to be structurally and functionally polarized. Differential functions may range from microtubule (MT) lattice binding and/or free tubulin heterodimer binding to potentiating stable incorporation of tubulin into the MT lattice.
Sequence similarities
Belongs to the TOG/XMAP215 family.
Keywords
- Domain
Phylogenomic databases
Family and domain databases
Sequence
- Sequence statusComplete
- Length1,415
- Mass (Da)156,189
- Last updated2011-12-14 v1
- Checksum9518FFA233ABB3A6
Features
Showing features for compositional bias.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Compositional bias | 251-277 | Polar residues | ||||
Sequence: PPVAPTSSTPSASAASGDPSGGTATAV | ||||||
Compositional bias | 572-596 | Basic and acidic residues | ||||
Sequence: EPLKPPPGDKKKKVPVKENEENEPP | ||||||
Compositional bias | 882-897 | Polar residues | ||||
Sequence: PKILVPQQQGSVVRRP | ||||||
Compositional bias | 898-914 | Basic and acidic residues | ||||
Sequence: ASRSRTREPEPEEVQSD |
Keywords
- Technical term
Sequence databases
Nucleotide Sequence | Protein Sequence | Molecule Type | Status | |
---|---|---|---|---|
AF035197 EMBL· GenBank· DDBJ | AAC17865.1 EMBL· GenBank· DDBJ | mRNA | ||
Z50044 EMBL· GenBank· DDBJ | CAA90359.1 EMBL· GenBank· DDBJ | Genomic DNA |