G5EEI8 · CHRD1_CAEEL
- ProteinCysteine and histidine-rich domain-containing protein 1
- Genechp-1
- StatusUniProtKB reviewed (Swiss-Prot)
- Organism
- Amino acids321 (go to sequence)
- Protein existenceEvidence at transcript level
- Annotation score3/5
Function
function
Regulates centrosome duplication (By similarity).
Controls the secretion of the tyrosine kinase receptor let-23/EGFR from the endoplasmic reticulum and is required for the localization of let-23/EGFR to the plasma membrane of vulval precursor cells (PubMed:32053105).
It thus plays a role in positively regulating let/EGFR signaling, and anchor cell and vulval precursor cell alignment (PubMed:32053105).
Plays a role in vulval development and morphogenesis (PubMed:10571178, PubMed:32053105).
Controls the secretion of the tyrosine kinase receptor let-23/EGFR from the endoplasmic reticulum and is required for the localization of let-23/EGFR to the plasma membrane of vulval precursor cells (PubMed:32053105).
It thus plays a role in positively regulating let/EGFR signaling, and anchor cell and vulval precursor cell alignment (PubMed:32053105).
Plays a role in vulval development and morphogenesis (PubMed:10571178, PubMed:32053105).
Features
Showing features for binding site.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Binding site | 9 | Zn2+ 1 (UniProtKB | ChEBI) | ||||
Sequence: C | ||||||
Binding site | 14 | Zn2+ 1 (UniProtKB | ChEBI) | ||||
Sequence: C | ||||||
Binding site | 28 | Zn2+ 1 (UniProtKB | ChEBI) | ||||
Sequence: C | ||||||
Binding site | 31 | Zn2+ 2 (UniProtKB | ChEBI) | ||||
Sequence: H | ||||||
Binding site | 46 | Zn2+ 2 (UniProtKB | ChEBI) | ||||
Sequence: C | ||||||
Binding site | 47 | Zn2+ 2 (UniProtKB | ChEBI) | ||||
Sequence: C | ||||||
Binding site | 63 | Zn2+ 2 (UniProtKB | ChEBI) | ||||
Sequence: C | ||||||
Binding site | 68 | Zn2+ 1 (UniProtKB | ChEBI) | ||||
Sequence: H | ||||||
Binding site | 152 | Zn2+ 3 (UniProtKB | ChEBI) | ||||
Sequence: C | ||||||
Binding site | 157 | Zn2+ 3 (UniProtKB | ChEBI) | ||||
Sequence: C | ||||||
Binding site | 170 | Zn2+ 3 (UniProtKB | ChEBI) | ||||
Sequence: C | ||||||
Binding site | 173 | Zn2+ 4 (UniProtKB | ChEBI) | ||||
Sequence: H | ||||||
Binding site | 188 | Zn2+ 4 (UniProtKB | ChEBI) | ||||
Sequence: C | ||||||
Binding site | 189 | Zn2+ 4 (UniProtKB | ChEBI) | ||||
Sequence: C | ||||||
Binding site | 205 | Zn2+ 4 (UniProtKB | ChEBI) | ||||
Sequence: C | ||||||
Binding site | 210 | Zn2+ 3 (UniProtKB | ChEBI) | ||||
Sequence: H |
GO annotations
all annotations | all molecular function | virus receptor activity | dna binding | rna binding | cytoskeletal motor activity | catalytic activity | gtpase activity | structural molecule activity | transporter activity | cytoskeletal protein binding | lipid binding | cyclase activity | antioxidant activity | oxidoreductase activity | transferase activity | hydrolase activity | lyase activity | isomerase activity | ligase activity | protein tag activity | cargo receptor activity | histone binding | protein folding chaperone | translation regulator activity | nutrient reservoir activity | receptor ligand activity | molecular transducer activity | molecular adaptor activity | toxin activity | cell adhesion mediator activity | molecular function regulator activity | virus coreceptor activity | catalytic activity, acting on a protein | catalytic activity, acting on dna | catalytic activity, acting on rna | molecular carrier activity | transcription regulator activity | general transcription initiation factor activity | molecular sensor activity | molecular sequestering activity | atp-dependent activity | other molecular function | all biological process | mitotic cell cycle | cytokinesis | cytoplasmic translation | immune system process | muscle system process | circulatory system process | renal system process | respiratory system process | carbohydrate metabolic process | generation of precursor metabolites and energy | dna replication | dna repair | dna recombination | chromatin organization | dna-templated transcription | regulation of dna-templated transcription | trna metabolic process | protein folding | protein glycosylation | amino acid metabolic process | modified amino acid metabolic process | lipid metabolic process | vitamin metabolic process | sulfur compound metabolic process | intracellular protein transport | nucleocytoplasmic transport | autophagy | inflammatory response | mitochondrion organization | cytoskeleton organization | microtubule-based movement | peroxisome organization | lysosome organization | chromosome segregation | cell adhesion | establishment or maintenance of cell polarity | programmed cell death | photosynthesis | mrna metabolic process | snrna metabolic process | vesicle-mediated transport | reproductive process | digestive system process | signaling | cell differentiation | protein catabolic process | extracellular matrix organization | regulatory ncrna-mediated gene silencing | telomere organization | cell junction organization | wound healing | ribosome biogenesis | cilium organization | anatomical structure development | cell motility | nervous system process | endocrine process | protein maturation | transmembrane transport | nucleobase-containing small molecule metabolic process | hepaticobiliary system process | membrane organization | protein-containing complex assembly | cell wall organization or biogenesis | nitrogen cycle metabolic process | protein localization to plasma membrane | defense response to other organism | detoxification | meiotic nuclear division | mitotic nuclear division | mitochondrial gene expression | carbohydrate derivative metabolic process | other biological process | all cellular component | nuclear chromosome | extracellular region | extracellular space | cell wall | nucleus | nuclear envelope | nucleoplasm | chromosome | nucleolus | mitochondrion | lysosome | endosome | vacuole | peroxisome | endoplasmic reticulum | golgi apparatus | lipid droplet | microtubule organizing center | cytosol | ribosome | cytoskeleton | plasma membrane | cilium | plastid | thylakoid | external encapsulating structure | extracellular matrix | cytoplasmic vesicle | organelle | other cellular component | |||
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Aspect | Term | |
---|---|---|
Molecular Function | zinc ion binding | |
Biological Process | centrosome duplication |
Keywords
- Ligand
Names & Taxonomy
Protein names
- Recommended nameCysteine and histidine-rich domain-containing protein 1
- Alternative names
Gene names
Organism names
- Organism
- Strain
- Taxonomic lineageEukaryota > Metazoa > Ecdysozoa > Nematoda > Chromadorea > Rhabditida > Rhabditina > Rhabditomorpha > Rhabditoidea > Rhabditidae > Peloderinae > Caenorhabditis
Accessions
- Primary accessionG5EEI8
Proteomes
Organism-specific databases
Phenotypes & Variants
Disruption phenotype
RNAi-mediated knockdown results in reduced fertility and in high levels of embryonic lethality with none of the unhatched embryos reaching the comma stage of development (PubMed:10571178).
Among the 40% of embryos that hatch, 98% reach adulthood, but have a reduced brood size, and the remaining 2% do not survive beyond the L1 larval stage (PubMed:10571178).
Surviving adults have defects in vulval development, whereby 3-5% have a protruding vulva (Pvl) phenotype and 1% have a multivulva (Mvl) phenotype (PubMed:10571178).
Occasionally, some adults have one missing gonadal arm, but many adults have defects in the organization of the proximal arm of the gonad, which are filled with smaller cells instead of full sized oocytes (PubMed:10571178).
RNAi-mediated knockdown results in reduced plasma membrane localization and increased intracellular accumulation of let-23 in vulval precursor cells P6.p and their descendants (PubMed:32053105).
RNAi-mediated knockdown does not affect the membrane localization of lin-12, lin-18 or pat-3 (PubMed:32053105).
RNAi-mediated knockdown in vulval precursor cells results in the intracellular accumulation of let-23 in 31% of cases (PubMed:32053105).
Among the 40% of embryos that hatch, 98% reach adulthood, but have a reduced brood size, and the remaining 2% do not survive beyond the L1 larval stage (PubMed:10571178).
Surviving adults have defects in vulval development, whereby 3-5% have a protruding vulva (Pvl) phenotype and 1% have a multivulva (Mvl) phenotype (PubMed:10571178).
Occasionally, some adults have one missing gonadal arm, but many adults have defects in the organization of the proximal arm of the gonad, which are filled with smaller cells instead of full sized oocytes (PubMed:10571178).
RNAi-mediated knockdown results in reduced plasma membrane localization and increased intracellular accumulation of let-23 in vulval precursor cells P6.p and their descendants (PubMed:32053105).
RNAi-mediated knockdown does not affect the membrane localization of lin-12, lin-18 or pat-3 (PubMed:32053105).
RNAi-mediated knockdown in vulval precursor cells results in the intracellular accumulation of let-23 in 31% of cases (PubMed:32053105).
PTM/Processing
Features
Showing features for chain.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Chain | PRO_0000450441 | 1-321 | Cysteine and histidine-rich domain-containing protein 1 | |||
Sequence: MVDESKLQCYHKGCGLLFDPKENDNEACTYHPGGPYFHDAYKIWTCCDKKSTDFGTWMNYKGCTRGKHSNEKPVDIVKVAAVKEIRPEKEEDVIVWKGLNKSGKLDSKDATKRIEQNLNVEVTPGATAAIEKKLKEISEAAQSADIQIGAPCRNNGCSTEFDGSKNKENCQHHPGAAIFHEGMKYWSCCNKKTSNFGAFLEQVGCTSGEHKFRNNEIVSKFREDWFSSNGFVTINVYCRGALPETANIVSDGHTVRVSMKHGFGNASVDLDYDLWDEVIPEESRVVIGERKVEISLKQKHGTGWPRLKFDPELDAKNDEEA |
Proteomic databases
Expression
Gene expression databases
Structure
Family & Domains
Features
Showing features for domain.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Domain | 9-68 | CHORD 1 | ||||
Sequence: CYHKGCGLLFDPKENDNEACTYHPGGPYFHDAYKIWTCCDKKSTDFGTWMNYKGCTRGKH | ||||||
Domain | 152-210 | CHORD 2 | ||||
Sequence: CRNNGCSTEFDGSKNKENCQHHPGAAIFHEGMKYWSCCNKKTSNFGAFLEQVGCTSGEH | ||||||
Domain | 218-308 | CS | ||||
Sequence: VSKFREDWFSSNGFVTINVYCRGALPETANIVSDGHTVRVSMKHGFGNASVDLDYDLWDEVIPEESRVVIGERKVEISLKQKHGTGWPRLK |
Keywords
- Domain
Phylogenomic databases
Family and domain databases
Sequence
- Sequence statusComplete
- Length321
- Mass (Da)36,007
- Last updated2011-12-14 v1
- Checksum8FF2228055BE2FBE
Keywords
- Technical term
Sequence databases
Nucleotide Sequence | Protein Sequence | Molecule Type | Status | |
---|---|---|---|---|
AF192264 EMBL· GenBank· DDBJ | AAF18435.1 EMBL· GenBank· DDBJ | mRNA | ||
BX284601 EMBL· GenBank· DDBJ | CCD66204.1 EMBL· GenBank· DDBJ | Genomic DNA |