G5EBD7 · 6PGDH_GLUOX
- Protein6-phosphogluconate dehydrogenase, NAD(+)-dependent, decarboxylating
- StatusUniProtKB reviewed (Swiss-Prot)
- Amino acids332 (go to sequence)
- Protein existenceEvidence at protein level
- Annotation score4/5
Function
function
Catalyzes the oxidative decarboxylation of 6-phosphogluconate to ribulose 5-phosphate and CO2, with concomitant reduction of NAD to NADH.
Catalytic activity
- 6-phospho-D-gluconate + NAD+ = D-ribulose 5-phosphate + CO2 + NADH
Kinetics
KM | SUBSTRATE | pH | TEMPERATURE[C] | NOTES | EVIDENCE | |
---|---|---|---|---|---|---|
0.44 mM | 6-phosphogluconate | |||||
64 μM | NAD+ | |||||
440 μM | NADP+ |
Vmax | pH | TEMPERATURE[C] | NOTES | EVIDENCE | |
---|---|---|---|---|---|
41.7 μmol/min/mg |
kcat is 23 sec-1. Can use NAD or NADP, but has a clear preference for NAD in vivo.
Pathway
Carbohydrate degradation; pentose phosphate pathway.
Features
Showing features for binding site, active site.
Type | ID | Position(s) | Description | ||
---|---|---|---|---|---|
Binding site | 7-12 | NAD+ (UniProtKB | ChEBI) | |||
Binding site | 30-32 | NAD+ (UniProtKB | ChEBI) | |||
Binding site | 101 | NAD+ (UniProtKB | ChEBI) | |||
Binding site | 101 | substrate | |||
Binding site | 127 | substrate | |||
Binding site | 129 | substrate | |||
Active site | 197 | Proton acceptor | |||
Binding site | 200-201 | substrate | |||
Active site | 204 | Proton donor | |||
Binding site | 205 | substrate | |||
GO annotations
Aspect | Term | |
---|---|---|
Molecular Function | NADP binding | |
Molecular Function | phosphogluconate dehydrogenase (decarboxylating) activity | |
Biological Process | D-gluconate metabolic process | |
Biological Process | organic acid catabolic process | |
Biological Process | pentose-phosphate shunt |
Keywords
- Molecular function
- Biological process
- Ligand
Enzyme and pathway databases
Names & Taxonomy
Protein names
- Recommended name6-phosphogluconate dehydrogenase, NAD(+)-dependent, decarboxylating
- EC number
- Short names6PGDH
Gene names
Organism names
- Strain
- Taxonomic lineageBacteria > Pseudomonadota > Alphaproteobacteria > Rhodospirillales > Acetobacteraceae > Gluconobacter
Accessions
- Primary accessionG5EBD7
Proteomes
PTM/Processing
Features
Showing features for chain.
Type | ID | Position(s) | Description | ||
---|---|---|---|---|---|
Chain | PRO_0000434436 | 1-332 | 6-phosphogluconate dehydrogenase, NAD+-dependent, decarboxylating | ||
Interaction
Structure
Sequence
- Sequence statusComplete
- Length332
- Mass (Da)35,876
- Last updated2011-12-14 v1
- Checksum2D7466AEF76E8B08
Keywords
- Technical term
Sequence databases
Nucleotide Sequence | Protein Sequence | Molecule Type | Status | |
---|---|---|---|---|
CP000009 EMBL· GenBank· DDBJ | AAW61445.1 EMBL· GenBank· DDBJ | Genomic DNA |