G4ZHR2 · XEG1_PHYSP
- ProteinXyloglucan-specific endo-beta-1,4-glucanase 1
- GeneXEG1
- StatusUniProtKB reviewed (Swiss-Prot)
- Amino acids241 (go to sequence)
- Protein existenceEvidence at protein level
- Annotation score5/5
Function
function
Glycoside hydrolase that exhibits xyloglucanase activity (PubMed:26163574).
Acts as an important virulence factor during P.sojae infection but also acts as a pathogen-associated molecular pattern (PAMP) in soybean and solanaceous species, where it can trigger defense responses including cell death. XEG1-induced cell death can be suppressed by P.sojae RxLR effectors. The PAMP activity is independent of its xyloglucanase activity (PubMed:26163574).
XEG1 induces plant defense responses in a RLP kinase Serk3/Bak1-dependent manner in Nicotiana benthamiana. Moreover, the perception of XEG1 occurs independently of the perception of ethylene-inducing xylanase Eix2 in Tomato (PubMed:26163574).
With truncated paralog XLP1, is required to elevate apoplastic sugar during P.sojae infection (PubMed:28082413).
Acts as an important virulence factor during P.sojae infection but also acts as a pathogen-associated molecular pattern (PAMP) in soybean and solanaceous species, where it can trigger defense responses including cell death. XEG1-induced cell death can be suppressed by P.sojae RxLR effectors. The PAMP activity is independent of its xyloglucanase activity (PubMed:26163574).
XEG1 induces plant defense responses in a RLP kinase Serk3/Bak1-dependent manner in Nicotiana benthamiana. Moreover, the perception of XEG1 occurs independently of the perception of ethylene-inducing xylanase Eix2 in Tomato (PubMed:26163574).
With truncated paralog XLP1, is required to elevate apoplastic sugar during P.sojae infection (PubMed:28082413).
Catalytic activity
Activity regulation
The xyloglucanase activity is inhibited by the binding of the host apoplastic glucanase inhibitor GIP1.
Features
Showing features for active site.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Active site | 136 | |||||
Sequence: E | ||||||
Active site | 222 | |||||
Sequence: E |
GO annotations
Aspect | Term | |
---|---|---|
Cellular Component | extracellular region | |
Cellular Component | host cellular component | |
Molecular Function | cellulase activity | |
Molecular Function | xyloglucan-specific endo-beta-1,4-glucanase activity | |
Biological Process | polysaccharide catabolic process |
Keywords
- Molecular function
- Biological process
Enzyme and pathway databases
Names & Taxonomy
Protein names
- Recommended nameXyloglucan-specific endo-beta-1,4-glucanase 1
- EC number
- Alternative names
Gene names
Organism names
- Strain
- Taxonomic lineageEukaryota > Sar > Stramenopiles > Oomycota > Peronosporales > Peronosporaceae > Phytophthora
Accessions
- Primary accessionG4ZHR2
Proteomes
Phenotypes & Variants
Disruption phenotype
Severely reduces virulence.
Features
Showing features for mutagenesis.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Mutagenesis | 136 | Abolishes xyloglucan-degrading endoglucanase activity, but does not affect elicitor activity. | ||||
Sequence: E → D | ||||||
Mutagenesis | 222 | Abolishes xyloglucan-degrading endoglucanase activity, but does not affect elicitor activity. | ||||
Sequence: E → D |
Miscellaneous
PTM/Processing
Features
Showing features for signal, chain, glycosylation.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Signal | 1-19 | |||||
Sequence: MKGFFAGVVAAATLAVASA | ||||||
Chain | PRO_5003472095 | 20-241 | Xyloglucan-specific endo-beta-1,4-glucanase 1 | |||
Sequence: GDYCGQWDWAKSTNYIVYNNLWNKNAAASGSQCTGVDKISGSTIAWHTSYTWTGGAATEVKSYSNAALVFSKKQIKNIKSIPTKMKYSYSHSSGTFVADVSYDLFTSSTASGSNEYEIMIWLAAYGGAGPISSTGKAIATVTIGSNSFKLYKGPNGSTTVFSFVATKTITNFSADLQKFLSYLTKNQGLPSSQYLITLEAGTEPFVGTNAKMTVSSFSAAVN | ||||||
Glycosylation | 174 | N-linked (GlcNAc...) asparagine | ||||
Sequence: N | ||||||
Glycosylation | 190 | N-linked (GlcNAc...) asparagine | ||||
Sequence: N |
Keywords
- PTM
PTM databases
Expression
Induction
Expression is strongly induced within 30 minutes of infection of soybean and then slowly declines.
Interaction
Subunit
Interacts with host apoplastic glucanase inhibitor GIP1.
Protein-protein interaction databases
Structure
Family & Domains
Sequence similarities
Belongs to the glycosyl hydrolase 12 (cellulase H) family.
Keywords
- Domain
Phylogenomic databases
Family and domain databases
Sequence
- Sequence statusComplete
- Sequence processingThe displayed sequence is further processed into a mature form.
- Length241
- Mass (Da)25,500
- Last updated2012-01-25 v1
- Checksum2356A68E942DC665
Keywords
- Technical term
Sequence databases
Nucleotide Sequence | Protein Sequence | Molecule Type | Status | |
---|---|---|---|---|
JH159154 EMBL· GenBank· DDBJ | EGZ16757.1 EMBL· GenBank· DDBJ | Genomic DNA |