G4ZHR2 · XEG1_PHYSP

Function

function

Glycoside hydrolase that exhibits xyloglucanase activity (PubMed:26163574).
Acts as an important virulence factor during P.sojae infection but also acts as a pathogen-associated molecular pattern (PAMP) in soybean and solanaceous species, where it can trigger defense responses including cell death. XEG1-induced cell death can be suppressed by P.sojae RxLR effectors. The PAMP activity is independent of its xyloglucanase activity (PubMed:26163574).
XEG1 induces plant defense responses in a RLP kinase Serk3/Bak1-dependent manner in Nicotiana benthamiana. Moreover, the perception of XEG1 occurs independently of the perception of ethylene-inducing xylanase Eix2 in Tomato (PubMed:26163574).
With truncated paralog XLP1, is required to elevate apoplastic sugar during P.sojae infection (PubMed:28082413).

Catalytic activity

  • xyloglucan + H2O = xyloglucan oligosaccharides.
    EC:3.2.1.151 (UniProtKB | ENZYME | Rhea)

Activity regulation

The xyloglucanase activity is inhibited by the binding of the host apoplastic glucanase inhibitor GIP1.

Features

Showing features for active site.

TypeIDPosition(s)Description
Active site136
Active site222

GO annotations

AspectTerm
Cellular Componentextracellular region
Cellular Componenthost cellular component
Molecular Functioncellulase activity
Molecular Functionxyloglucan-specific endo-beta-1,4-glucanase activity
Biological Processpolysaccharide catabolic process

Keywords

Enzyme and pathway databases

Names & Taxonomy

Protein names

  • Recommended name
    Xyloglucan-specific endo-beta-1,4-glucanase 1
  • EC number
  • Alternative names
    • Glycoside hydrolase family 12 protein XEG1
      (GH12 protein XEG1
      )

Gene names

    • Name
      XEG1
    • ORF names
      PHYSODRAFT_559651

Organism names

Accessions

  • Primary accession
    G4ZHR2

Proteomes

Subcellular Location

Secreted
Host
Note: Targeted to the host apoplast in order to trigger cell death.

Keywords

Phenotypes & Variants

Disruption phenotype

Severely reduces virulence.

Features

Showing features for mutagenesis.

TypeIDPosition(s)Description
Mutagenesis136Abolishes xyloglucan-degrading endoglucanase activity, but does not affect elicitor activity.
Mutagenesis222Abolishes xyloglucan-degrading endoglucanase activity, but does not affect elicitor activity.

Miscellaneous

PTM/Processing

Features

Showing features for signal, chain, glycosylation.

TypeIDPosition(s)Description
Signal1-19
ChainPRO_500347209520-241Xyloglucan-specific endo-beta-1,4-glucanase 1
Glycosylation174N-linked (GlcNAc...) asparagine
Glycosylation190N-linked (GlcNAc...) asparagine

Keywords

PTM databases

Expression

Induction

Expression is strongly induced within 30 minutes of infection of soybean and then slowly declines.

Interaction

Subunit

Interacts with host apoplastic glucanase inhibitor GIP1.

Protein-protein interaction databases

Family & Domains

Sequence similarities

Keywords

Phylogenomic databases

Family and domain databases

Sequence

  • Sequence status
    Complete
  • Sequence processing
    The displayed sequence is further processed into a mature form.
  • Length
    241
  • Mass (Da)
    25,500
  • Last updated
    2012-01-25 v1
  • Checksum
    2356A68E942DC665
MKGFFAGVVAAATLAVASAGDYCGQWDWAKSTNYIVYNNLWNKNAAASGSQCTGVDKISGSTIAWHTSYTWTGGAATEVKSYSNAALVFSKKQIKNIKSIPTKMKYSYSHSSGTFVADVSYDLFTSSTASGSNEYEIMIWLAAYGGAGPISSTGKAIATVTIGSNSFKLYKGPNGSTTVFSFVATKTITNFSADLQKFLSYLTKNQGLPSSQYLITLEAGTEPFVGTNAKMTVSSFSAAVN

Keywords

Sequence databases

Nucleotide SequenceProtein SequenceMolecule TypeStatus
JH159154
EMBL· GenBank· DDBJ
EGZ16757.1
EMBL· GenBank· DDBJ
Genomic DNA

Genome annotation databases

Similar Proteins

Disclaimer

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