G4V4G3 · YGFX_SERS3

Function

function

Required for production of prodigiosin antibiotic (Pig); overproduction in ygfX, sdhE or double sdhE-ygfX deletion mutants leads to nearly 10-fold increased synthesis of Pig (PubMed:22474332, PubMed:23657679).
Overexpressing cells have somewhat altered cell length depending on growth phase (PubMed:23657679).
Does not function as a toxin; overexpression in the presence or absence of sdhE (the putative antitoxin) has no effect on growth rate (PubMed:23657679).

Pathway

Antibiotic biosynthesis; prodigiosin biosynthesis.

GO annotations

all annotationsall molecular functionvirus receptor activitydna bindingrna bindingcytoskeletal motor activitycatalytic activitygtpase activitystructural molecule activitytransporter activitycytoskeletal protein bindinglipid bindingcyclase activityantioxidant activityoxidoreductase activitytransferase activityhydrolase activitylyase activityisomerase activityligase activityprotein tag activitycargo receptor activityhistone bindingprotein folding chaperonetranslation regulator activitynutrient reservoir activityreceptor ligand activitymolecular transducer activitymolecular adaptor activitytoxin activitycell adhesion mediator activitymolecular function regulator activityvirus coreceptor activitycatalytic activity, acting on a proteincatalytic activity, acting on dnacatalytic activity, acting on rnamolecular carrier activitytranscription regulator activitygeneral transcription initiation factor activitymolecular sensor activitymolecular sequestering activityatp-dependent activityother molecular functionall biological processmitotic cell cyclecytokinesiscytoplasmic translationimmune system processmuscle system processcirculatory system processrenal system processrespiratory system processcarbohydrate metabolic processgeneration of precursor metabolites and energydna replicationdna repairdna recombinationchromatin organizationdna-templated transcriptionregulation of dna-templated transcriptiontrna metabolic processprotein foldingprotein glycosylationamino acid metabolic processmodified amino acid metabolic processlipid metabolic processvitamin metabolic processsulfur compound metabolic processintracellular protein transportnucleocytoplasmic transportautophagyinflammatory responsemitochondrion organizationcytoskeleton organizationmicrotubule-based movementperoxisome organizationlysosome organizationchromosome segregationcell adhesionestablishment or maintenance of cell polarityprogrammed cell deathphotosynthesismrna metabolic processsnrna metabolic processvesicle-mediated transportreproductive processdigestive system processsignalingcell differentiationprotein catabolic processextracellular matrix organizationregulatory ncrna-mediated gene silencingtelomere organizationcell junction organizationwound healingribosome biogenesiscilium organizationanatomical structure developmentcell motilitynervous system processendocrine processprotein maturationtransmembrane transportnucleobase-containing small molecule metabolic processhepaticobiliary system processmembrane organizationprotein-containing complex assemblycell wall organization or biogenesisnitrogen cycle metabolic processprotein localization to plasma membranedefense response to other organismdetoxificationmeiotic nuclear divisionmitotic nuclear divisionmitochondrial gene expressioncarbohydrate derivative metabolic processother biological processall cellular componentnuclear chromosomeextracellular regionextracellular spacecell wallnucleusnuclear envelopenucleoplasmchromosomenucleolusmitochondrionlysosomeendosomevacuoleperoxisomeendoplasmic reticulumgolgi apparatuslipid dropletmicrotubule organizing centercytosolribosomecytoskeletonplasma membraneciliumplastidthylakoidexternal encapsulating structureextracellular matrixcytoplasmic vesicleorganelleother cellular component
Cell color indicative of number of GO terms
AspectTerm
Cellular Componentplasma membrane
Biological Processantibiotic biosynthetic process

Keywords

Enzyme and pathway databases

Names & Taxonomy

Protein names

  • Recommended name
    Inner membrane protein YgfX

Gene names

    • Name
      ygfX
    • Synonyms
      cptA
    • ORF names
      Ser39006_01162

Organism names

Accessions

  • Primary accession
    G4V4G3
  • Secondary accessions
    • V3V7V7

Proteomes

Subcellular Location

Cell inner membrane
; Multi-pass membrane protein

Features

Showing features for topological domain, transmembrane.

TypeIDPosition(s)Description
Topological domain1-11Cytoplasmic
Transmembrane12-31Helical
Topological domain32-35Periplasmic
Transmembrane36-54Helical
Topological domain55-143Cytoplasmic

Keywords

Phenotypes & Variants

Disruption phenotype

No effect on aerobic growth on succinate or in LB. 90% decreased synthesis of prodigiosin antibiotic, decreased transcription of flavoprotein desaturase PigA (PubMed:22474332, PubMed:23657679).

Features

Showing features for mutagenesis.

TypeIDPosition(s)Description
Mutagenesis1-13Less Pig production than wild-type, protein not detectable when His-tagged.
Mutagenesis1-54Protein only partially membrane-associated, no Pig production, 100% SDH activity observed.
Mutagenesis34Very low Pig production, poor self-interaction, no longer interacts with SdhE, protein accumulates stably in membrane.
Mutagenesis117Very low Pig production, no self-interaction, no longer interacts with SdhE, protein accumulates stably in membrane.

PTM/Processing

Features

Showing features for chain.

TypeIDPosition(s)Description
ChainPRO_00004388881-143Inner membrane protein YgfX

Expression

Induction

Transcribed under both aerobic (with succinate or glucose) and anaerobic (with glycerol with or without fumarate) conditions (PubMed:24374335).
Part of the sdhE-ygfX operon (PubMed:22474332).

Interaction

Subunit

Interacts with itself, SdhE and as in E.coli FtsZ and MreB; other interactions are shown but require validation (PubMed:23657679).

Protein-protein interaction databases

Family & Domains

Domain

The intact transmembrane domain is required for Pig production, its replacement with 2 TMs from an E.coli membrane protein (MalF) does not allow Pig synthesis; although the chimeric protein is found in the membrane it does not self-associate.

Keywords

Phylogenomic databases

Family and domain databases

Sequence

  • Sequence status
    Complete
  • Length
    143
  • Mass (Da)
    17,276
  • Last updated
    2011-12-14 v1
  • Checksum
    104C73867ECC0EB9
MVQWQCNLRVSWRMQLFSLLAHGLLVLLILLAPWPDGYMSVWLGLVTLVMFGFIRSQRNIKSRHGEIVLYNENHLRWQHREWQITKRPWVLKNGILLSLRTTEGKGPRRRSLWLASDSMRNEEWRHLCHLLLQHKNWQSDRLM

Sequence caution

The sequence ESN63754.1 differs from that shown. Reason: Erroneous initiation Truncated N-terminus.

Keywords

Sequence databases

Nucleotide SequenceProtein SequenceMolecule TypeStatus
HE580157
EMBL· GenBank· DDBJ
CCD22036.1
EMBL· GenBank· DDBJ
Genomic DNA
CP025084
EMBL· GenBank· DDBJ
ESN63754.1
EMBL· GenBank· DDBJ
Genomic DNA Different initiation

Similar Proteins

Disclaimer

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