G4V4G3 · YGFX_SERS3
- ProteinInner membrane protein YgfX
- GeneygfX
- StatusUniProtKB reviewed (Swiss-Prot)
- Amino acids143 (go to sequence)
- Protein existenceEvidence at protein level
- Annotation score4/5
Function
function
Required for production of prodigiosin antibiotic (Pig); overproduction in ygfX, sdhE or double sdhE-ygfX deletion mutants leads to nearly 10-fold increased synthesis of Pig (PubMed:22474332, PubMed:23657679).
Overexpressing cells have somewhat altered cell length depending on growth phase (PubMed:23657679).
Does not function as a toxin; overexpression in the presence or absence of sdhE (the putative antitoxin) has no effect on growth rate (PubMed:23657679).
Overexpressing cells have somewhat altered cell length depending on growth phase (PubMed:23657679).
Does not function as a toxin; overexpression in the presence or absence of sdhE (the putative antitoxin) has no effect on growth rate (PubMed:23657679).
Pathway
Antibiotic biosynthesis; prodigiosin biosynthesis.
GO annotations
all annotations | all molecular function | virus receptor activity | dna binding | rna binding | cytoskeletal motor activity | catalytic activity | gtpase activity | structural molecule activity | transporter activity | cytoskeletal protein binding | lipid binding | cyclase activity | antioxidant activity | oxidoreductase activity | transferase activity | hydrolase activity | lyase activity | isomerase activity | ligase activity | protein tag activity | cargo receptor activity | histone binding | protein folding chaperone | translation regulator activity | nutrient reservoir activity | receptor ligand activity | molecular transducer activity | molecular adaptor activity | toxin activity | cell adhesion mediator activity | molecular function regulator activity | virus coreceptor activity | catalytic activity, acting on a protein | catalytic activity, acting on dna | catalytic activity, acting on rna | molecular carrier activity | transcription regulator activity | general transcription initiation factor activity | molecular sensor activity | molecular sequestering activity | atp-dependent activity | other molecular function | all biological process | mitotic cell cycle | cytokinesis | cytoplasmic translation | immune system process | muscle system process | circulatory system process | renal system process | respiratory system process | carbohydrate metabolic process | generation of precursor metabolites and energy | dna replication | dna repair | dna recombination | chromatin organization | dna-templated transcription | regulation of dna-templated transcription | trna metabolic process | protein folding | protein glycosylation | amino acid metabolic process | modified amino acid metabolic process | lipid metabolic process | vitamin metabolic process | sulfur compound metabolic process | intracellular protein transport | nucleocytoplasmic transport | autophagy | inflammatory response | mitochondrion organization | cytoskeleton organization | microtubule-based movement | peroxisome organization | lysosome organization | chromosome segregation | cell adhesion | establishment or maintenance of cell polarity | programmed cell death | photosynthesis | mrna metabolic process | snrna metabolic process | vesicle-mediated transport | reproductive process | digestive system process | signaling | cell differentiation | protein catabolic process | extracellular matrix organization | regulatory ncrna-mediated gene silencing | telomere organization | cell junction organization | wound healing | ribosome biogenesis | cilium organization | anatomical structure development | cell motility | nervous system process | endocrine process | protein maturation | transmembrane transport | nucleobase-containing small molecule metabolic process | hepaticobiliary system process | membrane organization | protein-containing complex assembly | cell wall organization or biogenesis | nitrogen cycle metabolic process | protein localization to plasma membrane | defense response to other organism | detoxification | meiotic nuclear division | mitotic nuclear division | mitochondrial gene expression | carbohydrate derivative metabolic process | other biological process | all cellular component | nuclear chromosome | extracellular region | extracellular space | cell wall | nucleus | nuclear envelope | nucleoplasm | chromosome | nucleolus | mitochondrion | lysosome | endosome | vacuole | peroxisome | endoplasmic reticulum | golgi apparatus | lipid droplet | microtubule organizing center | cytosol | ribosome | cytoskeleton | plasma membrane | cilium | plastid | thylakoid | external encapsulating structure | extracellular matrix | cytoplasmic vesicle | organelle | other cellular component | |||
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Aspect | Term | |
---|---|---|
Cellular Component | plasma membrane | |
Biological Process | antibiotic biosynthetic process |
Keywords
- Biological process
Enzyme and pathway databases
Names & Taxonomy
Protein names
- Recommended nameInner membrane protein YgfX
Gene names
Organism names
- Strain
- Taxonomic lineageBacteria > Pseudomonadota > Gammaproteobacteria > Enterobacterales > Pectobacteriaceae > Prodigiosinella
Accessions
- Primary accessionG4V4G3
- Secondary accessions
Proteomes
Subcellular Location
UniProt Annotation
GO Annotation
Cell inner membrane ; Multi-pass membrane protein
Features
Showing features for topological domain, transmembrane.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Topological domain | 1-11 | Cytoplasmic | ||||
Sequence: MVQWQCNLRVS | ||||||
Transmembrane | 12-31 | Helical | ||||
Sequence: WRMQLFSLLAHGLLVLLILL | ||||||
Topological domain | 32-35 | Periplasmic | ||||
Sequence: APWP | ||||||
Transmembrane | 36-54 | Helical | ||||
Sequence: DGYMSVWLGLVTLVMFGFI | ||||||
Topological domain | 55-143 | Cytoplasmic | ||||
Sequence: RSQRNIKSRHGEIVLYNENHLRWQHREWQITKRPWVLKNGILLSLRTTEGKGPRRRSLWLASDSMRNEEWRHLCHLLLQHKNWQSDRLM |
Keywords
- Cellular component
Phenotypes & Variants
Disruption phenotype
No effect on aerobic growth on succinate or in LB. 90% decreased synthesis of prodigiosin antibiotic, decreased transcription of flavoprotein desaturase PigA (PubMed:22474332, PubMed:23657679).
Features
Showing features for mutagenesis.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Mutagenesis | 1-13 | Less Pig production than wild-type, protein not detectable when His-tagged. | ||||
Sequence: Missing | ||||||
Mutagenesis | 1-54 | Protein only partially membrane-associated, no Pig production, 100% SDH activity observed. | ||||
Sequence: Missing | ||||||
Mutagenesis | 34 | Very low Pig production, poor self-interaction, no longer interacts with SdhE, protein accumulates stably in membrane. | ||||
Sequence: W → A | ||||||
Mutagenesis | 117 | Very low Pig production, no self-interaction, no longer interacts with SdhE, protein accumulates stably in membrane. | ||||
Sequence: D → A |
PTM/Processing
Features
Showing features for chain.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Chain | PRO_0000438888 | 1-143 | Inner membrane protein YgfX | |||
Sequence: MVQWQCNLRVSWRMQLFSLLAHGLLVLLILLAPWPDGYMSVWLGLVTLVMFGFIRSQRNIKSRHGEIVLYNENHLRWQHREWQITKRPWVLKNGILLSLRTTEGKGPRRRSLWLASDSMRNEEWRHLCHLLLQHKNWQSDRLM |
Structure
Family & Domains
Domain
The intact transmembrane domain is required for Pig production, its replacement with 2 TMs from an E.coli membrane protein (MalF) does not allow Pig synthesis; although the chimeric protein is found in the membrane it does not self-associate.
Keywords
- Domain
Phylogenomic databases
Family and domain databases
Sequence
- Sequence statusComplete
- Length143
- Mass (Da)17,276
- Last updated2011-12-14 v1
- Checksum104C73867ECC0EB9
Sequence caution
Keywords
- Technical term