G4RK44 · TPSP_THETK

Function

function

Bifunctional enzyme which catalyzes the transfer of glucose from UDP-alpha-D-glucose to glucose-6-phosphate to form trehalose-6-phosphate (Tre6P) and removes the phosphate from Tre6P to produce free trehalose.

Miscellaneous

Part of an operon with putative glycosyltransferase TTX_1305 and putative membrane protein TTX_1304.1.

Catalytic activity

Cofactor

Mg2+ (UniProtKB | Rhea| CHEBI:18420 )

Temperature Dependence

Active at 80 degrees Celsius.

Pathway

Glycan biosynthesis; trehalose biosynthesis.

Features

Showing features for binding site, active site.

TypeIDPosition(s)Description
Binding site9D-glucose 6-phosphate (UniProtKB | ChEBI)
Binding site25-26UDP-alpha-D-glucose (UniProtKB | ChEBI)
Binding site89D-glucose 6-phosphate (UniProtKB | ChEBI)
Binding site143D-glucose 6-phosphate (UniProtKB | ChEBI)
Binding site276UDP-alpha-D-glucose (UniProtKB | ChEBI)
Binding site281UDP-alpha-D-glucose (UniProtKB | ChEBI)
Binding site314D-glucose 6-phosphate (UniProtKB | ChEBI)
Binding site379-383UDP-alpha-D-glucose (UniProtKB | ChEBI)
Active site503Nucleophile
Binding site503Mg2+ (UniProtKB | ChEBI)
Binding site503-505alpha,alpha-trehalose 6-phosphate (UniProtKB | ChEBI)
Binding site505Mg2+ (UniProtKB | ChEBI)
Binding site684Mg2+ (UniProtKB | ChEBI)

GO annotations

AspectTerm
Molecular Functionalpha,alpha-trehalose-phosphate synthase (UDP-forming) activity
Molecular Functionmetal ion binding
Molecular Functiontrehalose-phosphatase activity
Biological Processtrehalose biosynthetic process

Keywords

Enzyme and pathway databases

Protein family/group databases

Names & Taxonomy

Protein names

  • Recommended name
    Bifunctional trehalose-6-phosphate synthase/phosphatase

Including 2 domains:

  • Recommended name
    Alpha,alpha-trehalose-phosphate synthase [UDP-forming]
  • EC number
  • Alternative names
    • Trehalose-6-phosphate synthase
  • Recommended name
    Trehalose-6-phosphate phosphatase
  • EC number

Gene names

    • Name
      tpsp
    • Ordered locus names
      TTX_1304

Organism names

Accessions

  • Primary accession
    G4RK44
  • Secondary accessions
    • Q704C6

Proteomes

PTM/Processing

Features

Showing features for chain.

TypeIDPosition(s)Description
ChainPRO_00004245421-731Bifunctional trehalose-6-phosphate synthase/phosphatase

Proteomic databases

Interaction

Subunit

May interact with the putative glycosyltransferase (GT) TTX_1305. TTX_1305 is required for the trehalose-6-phosphate synthase activity of tpsp.

Protein-protein interaction databases

Structure

Family & Domains

Features

Showing features for region.

TypeIDPosition(s)Description
Region1-464Alpha,alpha-trehalose-phosphate synthase
Region465-731Trehalose-6-phosphate phosphatase

Sequence similarities

In the N-terminal section; belongs to the glycosyltransferase 20 family.
In the C-terminal section; belongs to the trehalose phosphatase family.

Phylogenomic databases

Family and domain databases

Sequence

  • Sequence status
    Complete
  • Length
    731
  • Mass (Da)
    81,699
  • Last updated
    2011-12-14 v1
  • Checksum
    D51D6D0B97A49FE4
MRLIVVSNRLPVTISPSGEIRESVGGLATAMKSFLGAVNGGRELGLEEVVWVGWSGVPSERESNDLRERLRGMGLEPVPLSSEEVEGFYEGFSNSTLWPLFHGFSEYATYEEKHWRAYRGVNEKYAKAVVALARPGDLVWIHDYHLMLAPAIVREAAEVGVGFFLHIPFPPAELLQLLPSEWRREILEGLLGSDLVGFHTYEYSANFSRSVVRFLGYKVEMGAIAVGHRRVRVGVFPIGIDFDRFYNSSQDPSVVEEMAKLREMLGRAKVVFSIDRLDYTKGVLRRVAAWERFLREHPEWRGRAVFVLVVVPSRTGVPMYEEMKRQIDREVGRINGELGELNWVPIVYLYRFIPSPTLMALYNIADVALITPLRDGMNLVAKEFVASKRDCRGVLILSELAGASKELAEALVINPNDVGGTAEAIAEALSMSEDEQCRRIRAMQERLRMRDVVRWGTDFIYSLISAKSAREEVEKALRYMEELSVDKLKSDFAKAKRRLLLLDYDGTLVPHYPYPHMAVPDGDLLELLSRLAALPETAVYVVSGRGRDFLDGWLGRLPVGLVAEHGFFLKHPGGEWKSLGKVDPSWRQYAKGIMEDFASNVPGSFVEVKEAGIAWHYRNADETIAEKAVVELIDALSNALAGSGLSILRGKKVVEVRPAGYTKGTAAKMLLDELSPDFVFVAGDDETDEGMFEVAPQSAYTVKVGPGPTLAKFRVGDYRGLRSLLEQLRPP

Sequence caution

The sequence CAF18468.1 differs from that shown. Reason: Erroneous initiation Extended N-terminus.

Keywords

Sequence databases

Nucleotide SequenceProtein SequenceMolecule TypeStatus
AJ621287
EMBL· GenBank· DDBJ
CAF18468.1
EMBL· GenBank· DDBJ
Genomic DNA Different initiation
FN869859
EMBL· GenBank· DDBJ
CCC81939.1
EMBL· GenBank· DDBJ
Genomic DNA

Genome annotation databases

Similar Proteins

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