G3RHK5 · G3RHK5_GORGO

Function

function

Catalyzes the methylation of 5-carboxymethyl uridine to 5-methylcarboxymethyl uridine at the wobble position of the anticodon loop in tRNA via its methyltransferase domain. Catalyzes the last step in the formation of 5-methylcarboxymethyl uridine at the wobble position of the anticodon loop in target tRNA. Has a preference for tRNA(Arg) and tRNA(Glu), and does not bind tRNA(Lys). Binds tRNA and catalyzes the iron and alpha-ketoglutarate dependent hydroxylation of 5-methylcarboxymethyl uridine at the wobble position of the anticodon loop in tRNA via its dioxygenase domain, giving rise to 5-(S)-methoxycarbonylhydroxymethyluridine; has a preference for tRNA(Gly). Required for normal survival after DNA damage. May inhibit apoptosis and promote cell survival and angiogenesis.

Catalytic activity

Cofactor

Fe2+ (UniProtKB | Rhea| CHEBI:29033 )

GO annotations

AspectTerm
Cellular Componentcytoplasm
Cellular Componentcytosol
Cellular Componentnucleoplasm
Cellular Componentnucleus
Molecular Function2-oxoglutarate-dependent dioxygenase activity
Molecular FunctionS-adenosylmethionine-dependent methyltransferase activity
Molecular FunctiontRNA (5-carboxymethyluridine(34)-5-O)-methyltransferase activity
Molecular FunctiontRNA binding
Molecular Functionzinc ion binding
Biological ProcessDNA damage response
Biological ProcesstRNA methylation
Biological ProcesstRNA wobble uridine modification

Keywords

Enzyme and pathway databases

Names & Taxonomy

Protein names

  • Recommended name
    Alkylated DNA repair protein alkB homolog 8
  • EC number
  • Alternative names
    • Probable alpha-ketoglutarate-dependent dioxygenase ABH8
    • S-adenosyl-L-methionine-dependent tRNA methyltransferase ABH8
    • tRNA (carboxymethyluridine(34)-5-O)-methyltransferase ABH8

Gene names

    • Name
      ALKBH8

Organism names

Accessions

  • Primary accession
    G3RHK5

Proteomes

Subcellular Location

Keywords

Expression

Gene expression databases

Interaction

Protein-protein interaction databases

Family & Domains

Features

Showing features for domain, compositional bias, region.

Type
IDPosition(s)Description
Domain220-337Fe2OG dioxygenase
Compositional bias515-534Basic and acidic residues
Region515-574Disordered
Compositional bias556-574Polar residues

Sequence similarities

Belongs to the alkB family.

Phylogenomic databases

Family and domain databases

Sequence

  • Sequence status
    Complete
  • Length
    664
  • Mass (Da)
    75,109
  • Last updated
    2018-02-28 v2
  • Checksum
    6F902FC4A914DBD7
MDSNHQSNYKLSKTEKKFLRKQIKAKHTLLRHEGIETVSYATQSLVVANGGLGNGVSRNQLLPVLEKCGLVDALLMPPNKPYSFARYRTTEESKRAYVTLNGKEVVDDLGQKIILYLNFVEKVQWKELRPQALPPGLMVVEEIISSEEEKMLLESVDWTEDTDNQNSQKSLKHRRVKHFGYEFHYENNNVDKDKPLSGGLPDICDSFLEKWLREGYIKHKPDQMTINQYEPGQGIPAHIDTHSAFEDEIVSLSLGSEIVMDFKHPDGIAVPVMLPRRSLLVMTGESRYLWTHGITCRKFDTVQASESLKSGIITSDVGDLTLSKRGLRTSFTFRKVRQTPCNCSYPLVCDSQRKETPPSFPESDKEASRLEQEYVHQVYEEIAGHFSSTRHTPWPHIVEFLKALPSGSIVADIGCGNGKYLGINKELYMIGCDRSQNLVDICRERQFQAFVCDALAVPVRSGSCDACISIAVIHHFATAERRVAALQEIVRLLRPGGKALIYVWAMEQEYNKQKSKYLRGNRNSQGKKEEMNSDTSVQRSLVEQMPDMGSRDSAFSAPGINDSQEGGCNSRQVSNSKLPIHVNRTSFYSQDVLVPWHLKGNPDKGKPVEPFGPIGSQDPSPVFHRYYHVFREGELEAACRTVSDVRILQSYYDQGNWCVILQKA

Computationally mapped potential isoform sequences

There is 1 potential isoform mapped to this entry

View all
EntryEntry nameGene nameLength
A0A2I2ZN35A0A2I2ZN35_GORGOALKBH8224

Features

Showing features for compositional bias.

TypeIDPosition(s)Description
Compositional bias515-534Basic and acidic residues
Compositional bias556-574Polar residues

Keywords

Sequence databases

Nucleotide SequenceProtein SequenceMolecule TypeStatus
CABD030081405
EMBL· GenBank· DDBJ
-Genomic DNA No translation available.
CABD030081406
EMBL· GenBank· DDBJ
-Genomic DNA No translation available.
CABD030081407
EMBL· GenBank· DDBJ
-Genomic DNA No translation available.

Genome annotation databases

Similar Proteins

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