G3RHK5 · G3RHK5_GORGO
- ProteinAlkylated DNA repair protein alkB homolog 8
- GeneALKBH8
- StatusUniProtKB unreviewed (TrEMBL)
- Amino acids664 (go to sequence)
- Protein existenceInferred from homology
- Annotation score4/5
Function
function
Catalyzes the methylation of 5-carboxymethyl uridine to 5-methylcarboxymethyl uridine at the wobble position of the anticodon loop in tRNA via its methyltransferase domain. Catalyzes the last step in the formation of 5-methylcarboxymethyl uridine at the wobble position of the anticodon loop in target tRNA. Has a preference for tRNA(Arg) and tRNA(Glu), and does not bind tRNA(Lys). Binds tRNA and catalyzes the iron and alpha-ketoglutarate dependent hydroxylation of 5-methylcarboxymethyl uridine at the wobble position of the anticodon loop in tRNA via its dioxygenase domain, giving rise to 5-(S)-methoxycarbonylhydroxymethyluridine; has a preference for tRNA(Gly). Required for normal survival after DNA damage. May inhibit apoptosis and promote cell survival and angiogenesis.
Catalytic activity
- 5-(carboxymethyl)uridine34 in tRNA + S-adenosyl-L-methionine = 5-(2-methoxy-2-oxoethyl)uridine34 in tRNA + S-adenosyl-L-homocysteine
Cofactor
GO annotations
Aspect | Term | |
---|---|---|
Cellular Component | cytoplasm | |
Cellular Component | cytosol | |
Cellular Component | nucleoplasm | |
Cellular Component | nucleus | |
Molecular Function | 2-oxoglutarate-dependent dioxygenase activity | |
Molecular Function | S-adenosylmethionine-dependent methyltransferase activity | |
Molecular Function | tRNA (5-carboxymethyluridine(34)-5-O)-methyltransferase activity | |
Molecular Function | tRNA binding | |
Molecular Function | zinc ion binding | |
Biological Process | DNA damage response | |
Biological Process | tRNA methylation | |
Biological Process | tRNA wobble uridine modification |
Keywords
- Molecular function
- Ligand
Enzyme and pathway databases
Names & Taxonomy
Protein names
- Recommended nameAlkylated DNA repair protein alkB homolog 8
- EC number
- Alternative names
Gene names
Organism names
- Taxonomic lineageEukaryota > Metazoa > Chordata > Craniata > Vertebrata > Euteleostomi > Mammalia > Eutheria > Euarchontoglires > Primates > Haplorrhini > Catarrhini > Hominidae > Gorilla
Accessions
- Primary accessionG3RHK5
Proteomes
Expression
Gene expression databases
Interaction
Protein-protein interaction databases
Structure
Family & Domains
Features
Showing features for domain, compositional bias, region.
Type | ID | Position(s) | Description | ||
---|---|---|---|---|---|
Domain | 220-337 | Fe2OG dioxygenase | |||
Compositional bias | 515-534 | Basic and acidic residues | |||
Region | 515-574 | Disordered | |||
Compositional bias | 556-574 | Polar residues | |||
Sequence similarities
Belongs to the alkB family.
Phylogenomic databases
Family and domain databases
Sequence
- Sequence statusComplete
- Length664
- Mass (Da)75,109
- Last updated2018-02-28 v2
- Checksum6F902FC4A914DBD7
Computationally mapped potential isoform sequences
There is 1 potential isoform mapped to this entry
Entry | Entry name | Gene name | Length | ||
---|---|---|---|---|---|
A0A2I2ZN35 | A0A2I2ZN35_GORGO | ALKBH8 | 224 |
Features
Showing features for compositional bias.
Type | ID | Position(s) | Description | ||
---|---|---|---|---|---|
Compositional bias | 515-534 | Basic and acidic residues | |||
Compositional bias | 556-574 | Polar residues | |||
Keywords
- Technical term
Sequence databases
Nucleotide Sequence | Protein Sequence | Molecule Type | Status | |
---|---|---|---|---|
CABD030081405 EMBL· GenBank· DDBJ | - | Genomic DNA | No translation available. | |
CABD030081406 EMBL· GenBank· DDBJ | - | Genomic DNA | No translation available. | |
CABD030081407 EMBL· GenBank· DDBJ | - | Genomic DNA | No translation available. |