G3J9P0 · TAS1_CORMM
- ProteinHybrid PKS-NRPS synthetase TAS1
- GeneTAS1
- StatusUniProtKB reviewed (Swiss-Prot)
- Amino acids1582 (go to sequence)
- Protein existenceEvidence at protein level
- Annotation score3/5
Function
function
Hybrid PKS-NRPS synthetase that mediates the biosynthesis of the toxin tenuazonic acid (TeA), an inhibitor of protein biosynthesis on ribosomes by suppressing the release of new protein. TAS1 alone is sufficient for TeA synthesis via the condensation of isoleucine (Ile) with acetoacetyl-CoA by the N-terminal NRPS module and subsequent cyclization conducted by the C-terminal KS domain.
Catalytic activity
- acetoacetyl-CoA + L-isoleucine + ATP = tenuazonic acid + AMP + diphosphate + CoA + 2 H+This reaction proceeds in the forward direction.
Cofactor
Features
Showing features for active site.
Type | ID | Position(s) | Description | ||
---|---|---|---|---|---|
Active site | 1294 | For beta-ketoacyl synthase activity | |||
Active site | 1432 | For beta-ketoacyl synthase activity | |||
Active site | 1498 | For beta-ketoacyl synthase activity | |||
GO annotations
Aspect | Term | |
---|---|---|
Cellular Component | cytoplasm | |
Molecular Function | 3-oxoacyl-[acyl-carrier-protein] synthase activity | |
Molecular Function | ligase activity | |
Molecular Function | phosphopantetheine binding | |
Biological Process | amino acid activation for nonribosomal peptide biosynthetic process | |
Biological Process | fatty acid biosynthetic process | |
Biological Process | secondary metabolite biosynthetic process |
Keywords
- Molecular function
- Biological process
Enzyme and pathway databases
Names & Taxonomy
Protein names
- Recommended nameHybrid PKS-NRPS synthetase TAS1
- EC number
- Alternative names
Gene names
Organism names
- Strain
- Taxonomic lineageEukaryota > Fungi > Dikarya > Ascomycota > Pezizomycotina > Sordariomycetes > Hypocreomycetidae > Hypocreales > Cordycipitaceae > Cordyceps
Accessions
- Primary accessionG3J9P0
Proteomes
Organism-specific databases
Subcellular Location
UniProt Annotation
GO Annotation
PTM/Processing
Features
Showing features for chain.
Type | ID | Position(s) | Description | ||
---|---|---|---|---|---|
Chain | PRO_0000460255 | 1-1582 | Hybrid PKS-NRPS synthetase TAS1 | ||
Keywords
- PTM
Interaction
Protein-protein interaction databases
Family & Domains
Features
Showing features for region, domain, compositional bias.
Type | ID | Position(s) | Description | ||
---|---|---|---|---|---|
Region | 33-387 | Condensation (C) domain | |||
Region | 499-892 | Adenylation (A) domain | |||
Domain | 1015-1092 | Carrier | |||
Domain | 1127-1574 | Ketosynthase family 3 (KS3) | |||
Region | 1460-1485 | Disordered | |||
Compositional bias | 1462-1485 | Polar residues | |||
Domain
TAS1 has the following domain architecture: C-A-T-KS. The N-terminal NRPS module contains the condensation (C), adenylation (A), and thiolation (T) domains and catalyzes the formation of the amide linkage between the isoleucine (Ile) with acetoacetyl-CoA. The PKS portion of TAS1 has only a ketosynthase (KS) domain and this domain is indispensable for TAS1 activity by conducting the final cyclization step for tenuazonic acid release.
Sequence similarities
In the N-terminal section; belongs to the NRP synthetase family.
Phylogenomic databases
Family and domain databases
Sequence
- Sequence statusComplete
- Length1,582
- Mass (Da)168,065
- Last updated2011-11-16 v1
- Checksum6EF3CBA73D3EDCBD
Features
Showing features for compositional bias.
Type | ID | Position(s) | Description | ||
---|---|---|---|---|---|
Compositional bias | 1462-1485 | Polar residues | |||
Keywords
- Technical term
Sequence databases
Nucleotide Sequence | Protein Sequence | Molecule Type | Status | |
---|---|---|---|---|
JH126400 EMBL· GenBank· DDBJ | EGX94166.1 EMBL· GenBank· DDBJ | Genomic DNA |