G3CCC0 · CLDS_TOBAC

Function

function

Class-II terpene synthase that synthesizes 8-hydroxy-copalyl diphosphate. Involved in the biosynthesis of cis-abienol, a labdane diterpene that can be used as synthesis precursor of ambergris substitution fragance products.

Miscellaneous

The expression of both CPS2 and ABS is necessary and sufficient to catalyze the biosynthesis of cis-abienol from geranylgeranyl diphosphate in a heterologous system.

Catalytic activity

Cofactor

Mg2+ (UniProtKB | Rhea| CHEBI:18420 )

Pathway

Secondary metabolite biosynthesis; terpenoid biosynthesis.

Features

Showing features for binding site.

TypeIDPosition(s)Description
Binding site249substrate
Binding site382Mg2+ (UniProtKB | ChEBI)
Binding site384Mg2+ (UniProtKB | ChEBI)
Binding site468substrate

GO annotations

AspectTerm
Cellular Componentchloroplast
Molecular Functioncopal-8-ol diphosphate synthase activity
Molecular Functionhydro-lyase activity
Molecular Functionmagnesium ion binding
Molecular Functionterpene synthase activity
Biological Processcopal-8-ol diphosphate(3-) biosynthetic process
Biological Processgeranylgeranyl diphosphate catabolic process
Biological Processgibberellin biosynthetic process

Keywords

Enzyme and pathway databases

Names & Taxonomy

Protein names

  • Recommended name
    Copal-8-ol diphosphate hydratase, chloroplastic
  • EC number
  • Alternative names
    • 8-hydroxy-copalyl diphosphate synthase

Gene names

    • Name
      CPS2

Organism names

  • Taxonomic identifier
  • Taxonomic lineage
    Eukaryota > Viridiplantae > Streptophyta > Embryophyta > Tracheophyta > Spermatophyta > Magnoliopsida > eudicotyledons > Gunneridae > Pentapetalae > asterids > lamiids > Solanales > Solanaceae > Nicotianoideae > Nicotianeae > Nicotiana

Accessions

  • Primary accession
    G3CCC0

Proteomes

Subcellular Location

Keywords

PTM/Processing

Features

Showing features for transit peptide, chain.

TypeIDPosition(s)Description
Transit peptide1-24Chloroplast
ChainPRO_500079311625-802Copal-8-ol diphosphate hydratase, chloroplastic

Proteomic databases

Expression

Tissue specificity

Expressed specifically in the secretory cells of the glandular trichomes.

Interaction

Protein-protein interaction databases

Structure

Family & Domains

Features

Showing features for motif.

TypeIDPosition(s)Description
Motif382-385DXDD motif

Domain

The Asp-Xaa-Asp-Asp (DXDD) motif is important for the catalytic activity, presumably through binding to Mg2+.

Sequence similarities

Belongs to the terpene synthase family.

Keywords

Family and domain databases

Sequence

  • Sequence status
    Complete
  • Sequence processing
    The displayed sequence is further processed into a mature form.
  • Length
    802
  • Mass (Da)
    93,245
  • Last updated
    2011-11-16 v1
  • Checksum
    B20B8BA346E67D27
MQVIITSSHRFFCHHLHQLKSPTSLSAQKAEFKKHGPRNWLFQTEGSLLYKPVRLNCATSDASYLGNVNEYLESDHSKNSEEKDIQVSRTIQMKGLTEEIKHMLNSMEDGRLNVLAYDTAWVSFIPNTTNNGNDQRPMFPSCLQWIIDNQLSDGSWGEEIVFCIYDRLLNTLVCVIALTLWNTCLHKRNKGVMFIKENLSKLETGEVENMTSGFELVFPTLLEKAQQLDIDIPYDAPVLKDIYARREVKLTRIPKDVIHTIPTTVLFSLEGLRDDLDWQRLLKLQMPDGSFLISPASTAFAFMETNDEKCLAYLQNVVEKSNGGARQYPFDLVTRLWAIDRLQRLGISYYFAEEFKELLNHVFRYWDEENGIFSGRNSNVSDVDDTCMAIRLLRLHGYDVSPDALNNFKDGDQFVCFRGEVDGSPTHMFNLYRCSQVLFPGEKILEEAKNFTYNFLQQCLANNRCLDKWVIAKDIPGEIWYALEFPWYASLPRVEARYYIEQYGGADDIWIGKTLYRMPDVNNNVYLQAAKLDYNRCQSQHRFEWLIMQEWFEKCNFQQFGISKKYLLVSYFLAAASIFEVEKSRERLAWAKSRIICKMITSYYNDEATTWTTRNSLLMEFKVSHDPTRKNGNETKEILVLKNLRQFLRQLSEETFEDLGKDIHHQLQNAWETWLVFLREEKNACQEETELLVRTINLSGGYMTHDEILFDADYENLSNLTNKVCGKLNELQNDKVTGGSKNTNIELDMQALVKLVFGNTSSNINQDIKQTFFAVVKTFYYSAHVSEEIMNFHISKVLFQQV

Keywords

Sequence databases

Nucleotide SequenceProtein SequenceMolecule TypeStatus
HE588139
EMBL· GenBank· DDBJ
CCD33018.1
EMBL· GenBank· DDBJ
Genomic DNA

Similar Proteins

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