G3CCC0 · CLDS_TOBAC
- ProteinCopal-8-ol diphosphate hydratase, chloroplastic
- GeneCPS2
- StatusUniProtKB reviewed (Swiss-Prot)
- Amino acids802 (go to sequence)
- Protein existenceEvidence at protein level
- Annotation score4/5
Function
function
Class-II terpene synthase that synthesizes 8-hydroxy-copalyl diphosphate. Involved in the biosynthesis of cis-abienol, a labdane diterpene that can be used as synthesis precursor of ambergris substitution fragance products.
Miscellaneous
The expression of both CPS2 and ABS is necessary and sufficient to catalyze the biosynthesis of cis-abienol from geranylgeranyl diphosphate in a heterologous system.
Catalytic activity
- (2E,6E,10E)-geranylgeranyl diphosphate + H2O = 8-hydroxycopalyl diphosphate
Cofactor
Pathway
Secondary metabolite biosynthesis; terpenoid biosynthesis.
Features
Showing features for binding site.
GO annotations
Aspect | Term | |
---|---|---|
Cellular Component | chloroplast | |
Molecular Function | copal-8-ol diphosphate synthase activity | |
Molecular Function | hydro-lyase activity | |
Molecular Function | magnesium ion binding | |
Molecular Function | terpene synthase activity | |
Biological Process | copal-8-ol diphosphate(3-) biosynthetic process | |
Biological Process | geranylgeranyl diphosphate catabolic process | |
Biological Process | gibberellin biosynthetic process |
Keywords
- Molecular function
- Ligand
Enzyme and pathway databases
Names & Taxonomy
Protein names
- Recommended nameCopal-8-ol diphosphate hydratase, chloroplastic
- EC number
- Alternative names
Gene names
Organism names
- Taxonomic lineageEukaryota > Viridiplantae > Streptophyta > Embryophyta > Tracheophyta > Spermatophyta > Magnoliopsida > eudicotyledons > Gunneridae > Pentapetalae > asterids > lamiids > Solanales > Solanaceae > Nicotianoideae > Nicotianeae > Nicotiana
Accessions
- Primary accessionG3CCC0
Proteomes
Subcellular Location
PTM/Processing
Features
Showing features for transit peptide, chain.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Transit peptide | 1-24 | Chloroplast | ||||
Sequence: MQVIITSSHRFFCHHLHQLKSPTS | ||||||
Chain | PRO_5000793116 | 25-802 | Copal-8-ol diphosphate hydratase, chloroplastic | |||
Sequence: LSAQKAEFKKHGPRNWLFQTEGSLLYKPVRLNCATSDASYLGNVNEYLESDHSKNSEEKDIQVSRTIQMKGLTEEIKHMLNSMEDGRLNVLAYDTAWVSFIPNTTNNGNDQRPMFPSCLQWIIDNQLSDGSWGEEIVFCIYDRLLNTLVCVIALTLWNTCLHKRNKGVMFIKENLSKLETGEVENMTSGFELVFPTLLEKAQQLDIDIPYDAPVLKDIYARREVKLTRIPKDVIHTIPTTVLFSLEGLRDDLDWQRLLKLQMPDGSFLISPASTAFAFMETNDEKCLAYLQNVVEKSNGGARQYPFDLVTRLWAIDRLQRLGISYYFAEEFKELLNHVFRYWDEENGIFSGRNSNVSDVDDTCMAIRLLRLHGYDVSPDALNNFKDGDQFVCFRGEVDGSPTHMFNLYRCSQVLFPGEKILEEAKNFTYNFLQQCLANNRCLDKWVIAKDIPGEIWYALEFPWYASLPRVEARYYIEQYGGADDIWIGKTLYRMPDVNNNVYLQAAKLDYNRCQSQHRFEWLIMQEWFEKCNFQQFGISKKYLLVSYFLAAASIFEVEKSRERLAWAKSRIICKMITSYYNDEATTWTTRNSLLMEFKVSHDPTRKNGNETKEILVLKNLRQFLRQLSEETFEDLGKDIHHQLQNAWETWLVFLREEKNACQEETELLVRTINLSGGYMTHDEILFDADYENLSNLTNKVCGKLNELQNDKVTGGSKNTNIELDMQALVKLVFGNTSSNINQDIKQTFFAVVKTFYYSAHVSEEIMNFHISKVLFQQV |
Proteomic databases
Expression
Tissue specificity
Expressed specifically in the secretory cells of the glandular trichomes.
Interaction
Protein-protein interaction databases
Structure
Family & Domains
Features
Showing features for motif.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Motif | 382-385 | DXDD motif | ||||
Sequence: DVDD |
Domain
The Asp-Xaa-Asp-Asp (DXDD) motif is important for the catalytic activity, presumably through binding to Mg2+.
Sequence similarities
Belongs to the terpene synthase family.
Keywords
- Domain
Family and domain databases
Sequence
- Sequence statusComplete
- Sequence processingThe displayed sequence is further processed into a mature form.
- Length802
- Mass (Da)93,245
- Last updated2011-11-16 v1
- ChecksumB20B8BA346E67D27
Keywords
- Technical term