G2QND5 · FAEB_THET4
- ProteinFeruloyl esterase B
- GeneFae1a
- StatusUniProtKB reviewed (Swiss-Prot)
- Amino acids291 (go to sequence)
- Protein existenceEvidence at protein level
- Annotation score5/5
Function
function
Feruloyl esterase which acts in synergy with xylanases in degradation of plant cell walls. Hydrolyzes the ester linkage of hydroxycinnamic acids (ferulic acid (FA) and p-coumaric acid) and diferulates present in plant cell walls. Is active on substrates containing ferulic acid ester linked to the C-5 and C-2 linkages of arabinofuranose, while it was found capable of de-esterifying acetylated glucuronoxylans. Efficiently releases ferulic acid (FA) from destarched wheat bran when incubated with an M3 xylanase.
Catalytic activity
Kinetics
KM | SUBSTRATE | pH | TEMPERATURE[C] | NOTES | EVIDENCE | |
---|---|---|---|---|---|---|
0.27 mM | methyl ferulate (MFA) | |||||
0.26 mM | methyl p-coumarate (MpCA) | |||||
0.18 mM | methyl caffeate (MCA) | |||||
0.21 mM | methyl sinapate (MSA) | |||||
2.29 mM | nitrophenyl-5-O-trans-feruloyl-alpha-L-arabinofuranoside | |||||
2.79 mM | nitrophenyl-2-O-trans-feruloyl-alpha-L-arabinofuranoside | |||||
2.33 mM | ethyl ferulate | |||||
1.14 mM | n-propyl ferulate | |||||
1.4 mM | iso-propyl ferulate | |||||
0.74 mM | n-butyl ferulate | |||||
0.65 mM | iso-butyl ferulate | |||||
0.74 mM | 2-butyl ferulate |
pH Dependence
Optimum pH is 7.0.
Temperature Dependence
Optimum temperature is 50 degrees Celsius.
Features
Showing features for active site.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Active site | 136 | Charge relay system | ||||
Sequence: S |
GO annotations
Aspect | Term | |
---|---|---|
Cellular Component | extracellular region | |
Molecular Function | feruloyl esterase activity | |
Biological Process | xylan catabolic process |
Keywords
- Molecular function
- Biological process
Enzyme and pathway databases
Protein family/group databases
Names & Taxonomy
Protein names
- Recommended nameFeruloyl esterase B
- EC number
- Alternative names
Gene names
Organism names
- Strain
- Taxonomic lineageEukaryota > Fungi > Dikarya > Ascomycota > Pezizomycotina > Sordariomycetes > Sordariomycetidae > Sordariales > Chaetomiaceae > Thermothelomyces
Accessions
- Primary accessionG2QND5
Proteomes
Organism-specific databases
Subcellular Location
Phenotypes & Variants
Features
Showing features for mutagenesis.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Mutagenesis | 136 | Impairs catalytic activity. | ||||
Sequence: S → A |
PTM/Processing
Features
Showing features for signal, chain, glycosylation.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Signal | 1-18 | |||||
Sequence: MLVRSFLGFAVLAATCLA | ||||||
Chain | PRO_0000419269 | 19-291 | Feruloyl esterase B | |||
Sequence: ASLQEVTEFGDNPTNIQMYIYVPDQLDTNPPVIVALHPCGGSAQQWFSGTQLPSYADDNGFILIYPSTPHMSNCWDIQNPDTLTHGQGGDALGIVSMVNYTLDKHSGDSSRVYAMGFSSGGMMTNQLAGSYPDVFEAGAVYSGVAFGCAAGAESATPFSPNQTCAQGLQKTAQEWGDFVRNAYAGYTGRRPRMQIFHGLEDTLVRPQCAEEALKQWSNVLGVELTQEVSGVPSPGWTQKIYGDGTQLQGFFGQGIGHQSTVNEQQLLQWFGLI | ||||||
Glycosylation | 117 | N-linked (GlcNAc...) asparagine | ||||
Sequence: N | ||||||
Glycosylation | 179 | N-linked (GlcNAc...) asparagine | ||||
Sequence: N |
Keywords
- PTM
PTM databases
Structure
Family & Domains
Sequence similarities
Belongs to the carbohydrate esterase 1 (CE1) family. Feruloyl esterase type B subfamily.
Keywords
- Domain
Phylogenomic databases
Family and domain databases
Sequence
- Sequence statusComplete
- Sequence processingThe displayed sequence is further processed into a mature form.
- Length291
- Mass (Da)31,372
- Last updated2011-11-16 v1
- Checksum5CD9B663A8D6D025
Keywords
- Technical term
Sequence databases
Nucleotide Sequence | Protein Sequence | Molecule Type | Status | |
---|---|---|---|---|
CP003008 EMBL· GenBank· DDBJ | AEO62008.1 EMBL· GenBank· DDBJ | Genomic DNA |