G2QND5 · FAEB_THET4

Function

function

Feruloyl esterase which acts in synergy with xylanases in degradation of plant cell walls. Hydrolyzes the ester linkage of hydroxycinnamic acids (ferulic acid (FA) and p-coumaric acid) and diferulates present in plant cell walls. Is active on substrates containing ferulic acid ester linked to the C-5 and C-2 linkages of arabinofuranose, while it was found capable of de-esterifying acetylated glucuronoxylans. Efficiently releases ferulic acid (FA) from destarched wheat bran when incubated with an M3 xylanase.

Catalytic activity

  • feruloyl-polysaccharide + H2O = ferulate + polysaccharide.
    EC:3.1.1.73 (UniProtKB | ENZYME | Rhea)

Kinetics

KM SUBSTRATE pH TEMPERATURE[C] NOTES EVIDENCE
0.27 mMmethyl ferulate (MFA)
0.26 mMmethyl p-coumarate (MpCA)
0.18 mMmethyl caffeate (MCA)
0.21 mMmethyl sinapate (MSA)
2.29 mMnitrophenyl-5-O-trans-feruloyl-alpha-L-arabinofuranoside
2.79 mMnitrophenyl-2-O-trans-feruloyl-alpha-L-arabinofuranoside
2.33 mMethyl ferulate
1.14 mMn-propyl ferulate
1.4 mMiso-propyl ferulate
0.74 mMn-butyl ferulate
0.65 mMiso-butyl ferulate
0.74 mM2-butyl ferulate

pH Dependence

Optimum pH is 7.0.

Temperature Dependence

Optimum temperature is 50 degrees Celsius.

Features

Showing features for active site.

TypeIDPosition(s)Description
Active site136Charge relay system

GO annotations

AspectTerm
Cellular Componentextracellular region
Molecular Functionferuloyl esterase activity
Biological Processxylan catabolic process

Keywords

Enzyme and pathway databases

Protein family/group databases

Names & Taxonomy

Protein names

  • Recommended name
    Feruloyl esterase B
  • EC number
  • Alternative names
    • Cinnamoyl esterase
    • Ferulic acid esterase B (FAEB)

Gene names

    • Name
      Fae1a
    • ORF names
      MYCTH_96478

Organism names

Accessions

  • Primary accession
    G2QND5

Proteomes

Organism-specific databases

Subcellular Location

Keywords

Phenotypes & Variants

Features

Showing features for mutagenesis.

TypeIDPosition(s)Description
Mutagenesis136Impairs catalytic activity.

PTM/Processing

Features

Showing features for signal, chain, glycosylation.

TypeIDPosition(s)Description
Signal1-18
ChainPRO_000041926919-291Feruloyl esterase B
Glycosylation117N-linked (GlcNAc...) asparagine
Glycosylation179N-linked (GlcNAc...) asparagine

Keywords

PTM databases

Structure

Family & Domains

Sequence similarities

Keywords

Phylogenomic databases

Family and domain databases

Sequence

  • Sequence status
    Complete
  • Sequence processing
    The displayed sequence is further processed into a mature form.
  • Length
    291
  • Mass (Da)
    31,372
  • Last updated
    2011-11-16 v1
  • Checksum
    5CD9B663A8D6D025
MLVRSFLGFAVLAATCLAASLQEVTEFGDNPTNIQMYIYVPDQLDTNPPVIVALHPCGGSAQQWFSGTQLPSYADDNGFILIYPSTPHMSNCWDIQNPDTLTHGQGGDALGIVSMVNYTLDKHSGDSSRVYAMGFSSGGMMTNQLAGSYPDVFEAGAVYSGVAFGCAAGAESATPFSPNQTCAQGLQKTAQEWGDFVRNAYAGYTGRRPRMQIFHGLEDTLVRPQCAEEALKQWSNVLGVELTQEVSGVPSPGWTQKIYGDGTQLQGFFGQGIGHQSTVNEQQLLQWFGLI

Keywords

Sequence databases

Nucleotide SequenceProtein SequenceMolecule TypeStatus
CP003008
EMBL· GenBank· DDBJ
AEO62008.1
EMBL· GenBank· DDBJ
Genomic DNA

Genome annotation databases

Similar Proteins

Disclaimer

Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care. Our staff consists of biologists and biochemists that are not trained to give medical advice.
We'd like to inform you that we have updated our Privacy Notice to comply with Europe’s new General Data Protection Regulation (GDPR) that applies since 25 May 2018.
FeedbackHelp