G2Q5N0 · PEX12_THET4
- ProteinPeroxisome assembly protein 12
- GenePEX12
- StatusUniProtKB reviewed (Swiss-Prot)
- Amino acids439 (go to sequence)
- Protein existenceEvidence at protein level
- Annotation score4/5
Function
function
Component of a retrotranslocation channel required for peroxisome organization by mediating export of the PEX5 receptor from peroxisomes to the cytosol, thereby promoting PEX5 recycling (By similarity).
The retrotranslocation channel is composed of PEX2, PEX10 and PEX12; each subunit contributing transmembrane segments that coassemble into an open channel that specifically allows the passage of PEX5 through the peroxisomal membrane (By similarity).
PEX12 also regulates PEX5 recycling by activating the E3 ubiquitin-protein ligase activity of PEX10 (By similarity).
When PEX5 recycling is compromised, PEX12 stimulates PEX10-mediated polyubiquitination of PEX5, leading to its subsequent degradation (By similarity).
The retrotranslocation channel is composed of PEX2, PEX10 and PEX12; each subunit contributing transmembrane segments that coassemble into an open channel that specifically allows the passage of PEX5 through the peroxisomal membrane (By similarity).
PEX12 also regulates PEX5 recycling by activating the E3 ubiquitin-protein ligase activity of PEX10 (By similarity).
When PEX5 recycling is compromised, PEX12 stimulates PEX10-mediated polyubiquitination of PEX5, leading to its subsequent degradation (By similarity).
Pathway
Protein modification; protein ubiquitination.
Features
Showing features for binding site.
GO annotations
all annotations | all molecular function | virus receptor activity | dna binding | rna binding | cytoskeletal motor activity | catalytic activity | gtpase activity | structural molecule activity | transporter activity | cytoskeletal protein binding | lipid binding | cyclase activity | antioxidant activity | oxidoreductase activity | transferase activity | hydrolase activity | lyase activity | isomerase activity | ligase activity | protein tag activity | cargo receptor activity | histone binding | protein folding chaperone | translation regulator activity | nutrient reservoir activity | receptor ligand activity | molecular transducer activity | molecular adaptor activity | toxin activity | cell adhesion mediator activity | molecular function regulator activity | virus coreceptor activity | catalytic activity, acting on a protein | catalytic activity, acting on dna | catalytic activity, acting on rna | molecular carrier activity | transcription regulator activity | general transcription initiation factor activity | molecular sensor activity | molecular sequestering activity | atp-dependent activity | other molecular function | all biological process | mitotic cell cycle | cytokinesis | cytoplasmic translation | immune system process | muscle system process | circulatory system process | renal system process | respiratory system process | carbohydrate metabolic process | generation of precursor metabolites and energy | dna replication | dna repair | dna recombination | chromatin organization | dna-templated transcription | regulation of dna-templated transcription | trna metabolic process | protein folding | protein glycosylation | amino acid metabolic process | modified amino acid metabolic process | lipid metabolic process | vitamin metabolic process | sulfur compound metabolic process | intracellular protein transport | nucleocytoplasmic transport | autophagy | inflammatory response | mitochondrion organization | cytoskeleton organization | microtubule-based movement | peroxisome organization | lysosome organization | chromosome segregation | cell adhesion | establishment or maintenance of cell polarity | programmed cell death | photosynthesis | mrna metabolic process | snrna metabolic process | vesicle-mediated transport | reproductive process | digestive system process | signaling | cell differentiation | protein catabolic process | extracellular matrix organization | regulatory ncrna-mediated gene silencing | telomere organization | cell junction organization | wound healing | ribosome biogenesis | cilium organization | anatomical structure development | cell motility | nervous system process | endocrine process | protein maturation | transmembrane transport | nucleobase-containing small molecule metabolic process | hepaticobiliary system process | membrane organization | protein-containing complex assembly | cell wall organization or biogenesis | nitrogen cycle metabolic process | protein localization to plasma membrane | defense response to other organism | detoxification | meiotic nuclear division | mitotic nuclear division | mitochondrial gene expression | carbohydrate derivative metabolic process | other biological process | all cellular component | nuclear chromosome | extracellular region | extracellular space | cell wall | nucleus | nuclear envelope | nucleoplasm | chromosome | nucleolus | mitochondrion | lysosome | endosome | vacuole | peroxisome | endoplasmic reticulum | golgi apparatus | lipid droplet | microtubule organizing center | cytosol | ribosome | cytoskeleton | plasma membrane | cilium | plastid | thylakoid | external encapsulating structure | extracellular matrix | cytoplasmic vesicle | organelle | other cellular component | |||
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Aspect | Term | |
---|---|---|
Cellular Component | peroxisomal membrane | |
Molecular Function | zinc ion binding | |
Biological Process | protein import into peroxisome matrix, receptor recycling | |
Biological Process | protein ubiquitination |
Keywords
- Biological process
- Ligand
Enzyme and pathway databases
Names & Taxonomy
Protein names
- Recommended namePeroxisome assembly protein 12
- Alternative names
Gene names
Organism names
- Strain
- Taxonomic lineageEukaryota > Fungi > Dikarya > Ascomycota > Pezizomycotina > Sordariomycetes > Sordariomycetidae > Sordariales > Chaetomiaceae > Thermothelomyces
Accessions
- Primary accessionG2Q5N0
Proteomes
Organism-specific databases
Subcellular Location
UniProt Annotation
GO Annotation
Peroxisome membrane ; Multi-pass membrane protein
Features
Showing features for topological domain, transmembrane.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Topological domain | 1-17 | Peroxisomal matrix | ||||
Sequence: MEFIPALQARIDEHKPS | ||||||
Transmembrane | 18-45 | Helical; Name=TM1 | ||||
Sequence: LFELLSEQQLAALLPPTVRYLLTVLTQR | ||||||
Topological domain | 46-49 | Cytoplasmic | ||||
Sequence: YPRY | ||||||
Transmembrane | 50-74 | Helical; Name=TM2 | ||||
Sequence: LLRVLNSFDELYALAALVVERHYLR | ||||||
Topological domain | 75-115 | Peroxisomal matrix | ||||
Sequence: TRGGSFTEHFYGLKRERALAAEIPRASAAAPGAVRDALRLR | ||||||
Transmembrane | 116-149 | Helical; Name=TM3 | ||||
Sequence: PADVWRNLLVIVGVPYLKRKLDEAHEADAPRAMM | ||||||
Topological domain | 150-162 | Cytoplasmic | ||||
Sequence: GAAYNRPPLPGAP | ||||||
Transmembrane | 163-199 | Helical; Name=TM4 | ||||
Sequence: WRERVAFWWRCFLRRVYPAVNAAYYLSILAFNLAYLF | ||||||
Topological domain | 200-253 | Peroxisomal matrix | ||||
Sequence: DNSKYHSPFLCLIGTRVRRMSAADYRAIEALEERAAKRRGGRSVAARMLGGLSL | ||||||
Transmembrane | 254-281 | Helical; Name=TM5 | ||||
Sequence: VLPTSIFALKFLEWWYASDFAKQLSRKA | ||||||
Topological domain | 282-439 | Cytoplasmic | ||||
Sequence: AESLDLPPPVVAGLPTGAGGGGGSEKAQQPRREKELGKEKDEEVEEEEEEVSEEEKERRAIERAPVSASSLLPIYTVPPPENSDQCPICEGEITTAAACQTGIVYCYPCIHKWLTGTHPRQEKFMAERAGKWESGEGRCAVTGRRVLGGTEGLRRIMV |
Keywords
- Cellular component
PTM/Processing
Features
Showing features for chain.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Chain | PRO_0000456991 | 1-439 | Peroxisome assembly protein 12 | |||
Sequence: MEFIPALQARIDEHKPSLFELLSEQQLAALLPPTVRYLLTVLTQRYPRYLLRVLNSFDELYALAALVVERHYLRTRGGSFTEHFYGLKRERALAAEIPRASAAAPGAVRDALRLRPADVWRNLLVIVGVPYLKRKLDEAHEADAPRAMMGAAYNRPPLPGAPWRERVAFWWRCFLRRVYPAVNAAYYLSILAFNLAYLFDNSKYHSPFLCLIGTRVRRMSAADYRAIEALEERAAKRRGGRSVAARMLGGLSLVLPTSIFALKFLEWWYASDFAKQLSRKAAESLDLPPPVVAGLPTGAGGGGGSEKAQQPRREKELGKEKDEEVEEEEEEVSEEEKERRAIERAPVSASSLLPIYTVPPPENSDQCPICEGEITTAAACQTGIVYCYPCIHKWLTGTHPRQEKFMAERAGKWESGEGRCAVTGRRVLGGTEGLRRIMV |
Interaction
Subunit
Component of the PEX2-PEX10-PEX12 retrotranslocation channel, composed of PEX2, PEX10 and PEX12.
Protein-protein interaction databases
Structure
Family & Domains
Features
Showing features for region, zinc finger.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Region | 292-343 | Disordered | ||||
Sequence: VAGLPTGAGGGGGSEKAQQPRREKELGKEKDEEVEEEEEEVSEEEKERRAIE | ||||||
Zinc finger | 366-439 | RING-type; degenerate | ||||
Sequence: QCPICEGEITTAAACQTGIVYCYPCIHKWLTGTHPRQEKFMAERAGKWESGEGRCAVTGRRVLGGTEGLRRIMV |
Domain
The three subunits of the retrotranslocation channel (PEX2, PEX10 and PEX12) coassemble in the membrane into a channel with an open 10 Angstrom pore (PubMed:35768507).
The RING-type zinc-fingers that catalyze PEX5 receptor ubiquitination are positioned above the pore on the cytosolic side of the complex (PubMed:35768507).
The RING-type zinc-fingers that catalyze PEX5 receptor ubiquitination are positioned above the pore on the cytosolic side of the complex (PubMed:35768507).
The RING-type zinc-finger is degenerated and only coordinates one zinc ions, preventing E3 ubiquitin-protein ligase activity.
Sequence similarities
Belongs to the pex2/pex10/pex12 family.
Keywords
- Domain
Phylogenomic databases
Family and domain databases
Sequence
- Sequence statusComplete
- Length439
- Mass (Da)49,301
- Last updated2011-11-16 v1
- Checksum406DA4CAF6278E4C
Keywords
- Technical term
Sequence databases
Nucleotide Sequence | Protein Sequence | Molecule Type | Status | |
---|---|---|---|---|
CP003002 EMBL· GenBank· DDBJ | AEO55466.1 EMBL· GenBank· DDBJ | Genomic DNA |