G2IN77 · LIGXC_SPHSK
- Protein5,5'-dehydrodivanillate O-demethylase ferredoxin subunit
- GeneligXc
- StatusUniProtKB reviewed (Swiss-Prot)
- Amino acids105 (go to sequence)
- Protein existenceEvidence at protein level
- Annotation score4/5
Function
function
Involved in the catabolism of 5,5'-dehydrodivanillate (DDVA), an intermediate in the biodegradation of lignin. Part of a three-component monooxygenase that catalyzes the O-demethylation of DDVA, leading to the formation of 2,2',3-trihydroxy-3'-methoxy-5,5'-dicarboxybiphenyl (OH-DDVA). LigXc probably functions as an intermediate electron transfer protein between LigXd and LigXa.
Catalytic activity
- 5,5'-dehydrodivanillate + H+ + NADH + O2 = 2,2',3-trihydroxy-3'-methoxy-5,5'-dicarboxybiphenyl + formaldehyde + H2O + NAD+
Cofactor
Note: Binds 1 [2Fe-2S] cluster.
pH Dependence
Optimum pH is 6.0.
Temperature Dependence
Optimum temperature is 25-30 degrees Celsius.
Features
Showing features for binding site.
GO annotations
Aspect | Term | |
---|---|---|
Molecular Function | 2 iron, 2 sulfur cluster binding | |
Molecular Function | metal ion binding | |
Molecular Function | monooxygenase activity | |
Biological Process | P450-containing electron transport chain |
Keywords
- Molecular function
- Biological process
- Ligand
Enzyme and pathway databases
Names & Taxonomy
Protein names
- Recommended name5,5'-dehydrodivanillate O-demethylase ferredoxin subunit
- EC number
- Short namesDDVA O-demethylase ferredoxin subunit
Gene names
Organism names
- Strain
- Taxonomic lineageBacteria > Pseudomonadota > Alphaproteobacteria > Sphingomonadales > Sphingomonadaceae > Sphingobium
Accessions
- Primary accessionG2IN77
Proteomes
Phenotypes & Variants
Disruption phenotype
Disruption mutant shows growth defect on DDVA.
PTM/Processing
Features
Showing features for chain.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Chain | PRO_0000445224 | 1-105 | 5,5'-dehydrodivanillate O-demethylase ferredoxin subunit | |||
Sequence: MAQLKVVTRDGSLHEFEAPDGYTVMEAIRDQGIDELLAICGGCCSCATCHVFVEEAFLDKLPPLKGDEDDLLDSSDHRQANSRLSCQLPIGPELGGMTVTIAPED |
Interaction
Subunit
Monomer. The three-component monooxygenase is composed of an oxygenase (LigXa), a ferredoxin (LigXc) and a ferredoxin reductase (LigXd).
Protein-protein interaction databases
Structure
Family & Domains
Features
Showing features for domain.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Domain | 2-105 | 2Fe-2S ferredoxin-type | ||||
Sequence: AQLKVVTRDGSLHEFEAPDGYTVMEAIRDQGIDELLAICGGCCSCATCHVFVEEAFLDKLPPLKGDEDDLLDSSDHRQANSRLSCQLPIGPELGGMTVTIAPED |
Sequence similarities
Belongs to the adrenodoxin/putidaredoxin family.
Phylogenomic databases
Family and domain databases
Sequence
- Sequence statusComplete
- Length105
- Mass (Da)11,322
- Last updated2011-11-16 v1
- Checksum49CC09008DA2F981
Keywords
- Technical term
Sequence databases
Nucleotide Sequence | Protein Sequence | Molecule Type | Status | |
---|---|---|---|---|
AP012222 EMBL· GenBank· DDBJ | BAK65525.1 EMBL· GenBank· DDBJ | Genomic DNA |