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G2GZB5 · G2GZB5_9ENTR

Function

function

Specifically catalyzes the cleavage of the D-lactyl ether substituent of MurNAc 6-phosphate, producing GlcNAc 6-phosphate and D-lactate. Together with AnmK, is also required for the utilization of anhydro-N-acetylmuramic acid (anhMurNAc) either imported from the medium or derived from its own cell wall murein, and thus plays a role in cell wall recycling.

Miscellaneous

A lyase-type mechanism (elimination/hydration) is suggested for the cleavage of the lactyl ether bond of MurNAc 6-phosphate, with the formation of an alpha,beta-unsaturated aldehyde intermediate with (E)-stereochemistry, followed by the syn addition of water to give product.

Caution

The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.

Catalytic activity

Pathway

Amino-sugar metabolism; 1,6-anhydro-N-acetylmuramate degradation.
Amino-sugar metabolism; N-acetylmuramate degradation.
Cell wall biogenesis; peptidoglycan recycling.

Features

Showing features for active site.

TypeIDPosition(s)Description
Active site84Proton donor
Active site115

GO annotations

AspectTerm
Cellular Componentcytosol
Molecular Functioncarbohydrate binding
Molecular Functioncarbon-oxygen lyase activity
Molecular Functionenzyme binding
Molecular Functionenzyme inhibitor activity
Molecular Functionfructose-6-phosphate binding
Biological Process1,6-anhydro-N-acetyl-beta-muramic acid catabolic process
Biological Processamino sugar catabolic process
Biological Processglucose homeostasis
Biological ProcessN-acetylmuramic acid catabolic process
Biological Processnegative regulation of glucokinase activity
Biological Processpeptidoglycan turnover
Biological Processresponse to fructose

Keywords

Enzyme and pathway databases

Names & Taxonomy

Protein names

  • Recommended name
    N-acetylmuramic acid 6-phosphate etherase
  • EC number
  • Short names
    MurNAc-6-P etherase
  • Alternative names
    • N-acetylmuramic acid 6-phosphate hydrolase
    • N-acetylmuramic acid 6-phosphate lyase

Gene names

    • Name
      murQ
    • ORF names
      Rin_00011300

Organism names

  • Taxonomic identifier
  • Strain
    • R5.15
  • Taxonomic lineage
    Bacteria > Pseudomonadota > Gammaproteobacteria > Enterobacterales > Enterobacteriaceae > aphid secondary symbionts > Candidatus Regiella

Accessions

  • Primary accession
    G2GZB5

Proteomes

Subcellular Location

Expression

Induction

Induced by MurNAc 6-phosphate that releases the repressor MurR from the DNA. Repressed by MurR in the absence of MurNAc 6-phosphate.

Interaction

Subunit

Homodimer.

Family & Domains

Features

Showing features for domain.

TypeIDPosition(s)Description
Domain56-219SIS

Sequence similarities

Belongs to the GCKR-like family. MurNAc-6-P etherase subfamily.

Phylogenomic databases

Family and domain databases

Sequence

  • Sequence status
    Complete
  • Length
    301
  • Mass (Da)
    32,131
  • Last updated
    2011-11-16 v1
  • MD5 Checksum
    362AE1DA800859CFF1A4E84BAAFE78E3
MKNLYELTTESRNQASENIDMLSTIDMLKIINNEDKKVALAVEAVLPVIAQAVDCIVEAFQQGGRLIYCGAGTSGRLGILDASECPPTYGTPHEQVIALIAGGHAAIFQAVENAEDNQALALADLKKINFNAKDVLVGIAASGRTTYVIAALGYAKKLHAKTIALTCNAGSEVTQVADIAIVPIVGAEVVTGSSRMKAGTAQKLVLNMLTTGAMIRTGKVYGNLMVNVETKNLKLVQRQIDIVMQACGCNQDRAIQALQECNGDCKIAIVIILTALSVDEARVALQQNNGFIRRVLSEYRD

Keywords

Sequence databases

Nucleotide SequenceProtein SequenceMolecule TypeStatus
AGCA01000283
EMBL· GenBank· DDBJ
EGY28927.1
EMBL· GenBank· DDBJ
Genomic DNA

Genome annotation databases

Similar Proteins

Disclaimer

Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care. Our staff consists of biologists and biochemists that are not trained to give medical advice.
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