G2GW87 · G2GW87_9ENTR
- ProteinNADPH-dependent 7-cyano-7-deazaguanine reductase
- GenequeF
- StatusUniProtKB unreviewed (TrEMBL)
- Amino acids279 (go to sequence)
- Protein existenceInferred from homology
- Annotation score3/5
Function
function
Catalyzes the NADPH-dependent reduction of 7-cyano-7-deazaguanine (preQ0) to 7-aminomethyl-7-deazaguanine (preQ1).
Catalytic activity
- 7-aminomethyl-7-carbaguanine + 2 NADP+ = 7-cyano-7-deazaguanine + 2 NADPH + 3 H+
Pathway
tRNA modification; tRNA-queuosine biosynthesis.
Features
Showing features for binding site, active site.
Type | ID | Position(s) | Description | ||
---|---|---|---|---|---|
Binding site | 85-87 | substrate | |||
Binding site | 87-88 | NADPH (UniProtKB | ChEBI) | |||
Active site | 187 | Thioimide intermediate | |||
Active site | 194 | Proton donor | |||
Binding site | 226-227 | substrate | |||
Binding site | 255-256 | NADPH (UniProtKB | ChEBI) | |||
GO annotations
Aspect | Term | |
---|---|---|
Cellular Component | cytoplasm | |
Molecular Function | preQ1 synthase activity | |
Biological Process | queuosine biosynthetic process | |
Biological Process | tRNA modification |
Keywords
- Molecular function
- Biological process
- Ligand
Enzyme and pathway databases
Names & Taxonomy
Protein names
- Recommended nameNADPH-dependent 7-cyano-7-deazaguanine reductase
- EC number
- Alternative names
Gene names
Organism names
- Strain
- Taxonomic lineageBacteria > Pseudomonadota > Gammaproteobacteria > Enterobacterales > Enterobacteriaceae > aphid secondary symbionts > Candidatus Regiella
Accessions
- Primary accessionG2GW87
Proteomes
Subcellular Location
Interaction
Subunit
Homodimer.
Structure
Family & Domains
Features
Showing features for domain.
Type | ID | Position(s) | Description | ||
---|---|---|---|---|---|
Domain | 18-127 | NADPH-dependent 7-cyano-7-deazaguanine reductase N-terminal | |||
Sequence similarities
Belongs to the GTP cyclohydrolase I family. QueF type 2 subfamily.
Phylogenomic databases
Family and domain databases
Sequence
- Sequence statusComplete
- Length279
- Mass (Da)32,443
- Last updated2011-11-16 v1
- Checksum2CF682C574B68D10
Keywords
- Technical term
Sequence databases
Nucleotide Sequence | Protein Sequence | Molecule Type | Status | |
---|---|---|---|---|
AGCA01000005 EMBL· GenBank· DDBJ | EGY29991.1 EMBL· GenBank· DDBJ | Genomic DNA |