G1X2U7 · G1X2U7_ARTOA
- ProteinGlutamyl-tRNA(Gln) amidotransferase subunit A, mitochondrial
- StatusUniProtKB unreviewed (TrEMBL)
- Amino acids482 (go to sequence)
- Protein existenceInferred from homology
- Annotation score3/5
Function
function
Allows the formation of correctly charged Gln-tRNA(Gln) through the transamidation of misacylated Glu-tRNA(Gln) in the mitochondria. The reaction takes place in the presence of glutamine and ATP through an activated gamma-phospho-Glu-tRNA(Gln).
Catalytic activity
- L-glutamyl-tRNA(Gln) + L-glutamine + ATP + H2O = L-glutaminyl-tRNA(Gln) + L-glutamate + ADP + phosphate + H+
Features
Showing features for active site.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Active site | 64 | Charge relay system | ||||
Sequence: K | ||||||
Active site | 142 | Charge relay system | ||||
Sequence: S | ||||||
Active site | 166 | Acyl-ester intermediate | ||||
Sequence: S |
GO annotations
Aspect | Term | |
---|---|---|
Cellular Component | glutamyl-tRNA(Gln) amidotransferase complex | |
Cellular Component | mitochondrion | |
Molecular Function | amidase activity | |
Molecular Function | ATP binding | |
Molecular Function | glutaminyl-tRNA synthase (glutamine-hydrolyzing) activity | |
Molecular Function | indoleacetamide hydrolase activity | |
Biological Process | glutaminyl-tRNAGln biosynthesis via transamidation | |
Biological Process | mitochondrial translation |
Keywords
- Molecular function
- Biological process
- Ligand
Enzyme and pathway databases
Names & Taxonomy
Protein names
- Recommended nameGlutamyl-tRNA(Gln) amidotransferase subunit A, mitochondrial
- EC number
- Short namesGlu-AdT subunit A
Gene names
Organism names
- Strain
- Taxonomic lineageEukaryota > Fungi > Dikarya > Ascomycota > Pezizomycotina > Orbiliomycetes > Orbiliales > Orbiliaceae > Orbilia > Orbilia oligospora
Accessions
- Primary accessionG1X2U7
Proteomes
Subcellular Location
Interaction
Subunit
Subunit of the heterotrimeric GatCAB amidotransferase (AdT) complex, composed of A, B and C subunits.
Protein-protein interaction databases
Structure
Family & Domains
Features
Showing features for domain.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Domain | 12-464 | Amidase | ||||
Sequence: KYLRNIQQRNGFLNAFVRVSPEQAVTAQAIAASQKFETGKSISPLNGSTFAIKDNICTKDMVTSCSSLMLADFIPEYDSAVVAKLRDAGAIIIGKTNMDEFGMGSHSTTGLHGPVKNPNSSKLSVYSAGGSSGGSAAAVAGSLCFGALGSDTGGSVRLPASYCGVVGFKPSYGLLSRQGVIAYANSLDTVGILARDVHSAQLVFDCLNEYDPADPSSLDGHSRRKIQDHVAFPKPAANQSCRWNFGVPVDYNVVEISPQVKKAWISTLAILRDLGHNIKPVNMPSTRHAFGPHILAISKPANGISPSLQLVVAARERGFGKEVRRRILLGSYSLTSEAMENYFIHAQRIRRRIKEEFDGIFSSPNPLGPTQGSHLDPGASVDFLISPVSISSAPNLEEVTKVDASGVDGYINDVFTVPASMAGLPAISIPVHGAGGLPIGLQVTAQYGDEKNL |
Sequence similarities
Belongs to the amidase family. GatA subfamily.
Phylogenomic databases
Family and domain databases
Sequence
- Sequence statusComplete
- Length482
- Mass (Da)51,144
- Last updated2011-11-16 v1
- ChecksumF66CFD860653697E
Keywords
- Technical term
Sequence databases
Nucleotide Sequence | Protein Sequence | Molecule Type | Status | |
---|---|---|---|---|
ADOT01000056 EMBL· GenBank· DDBJ | EGX52526.1 EMBL· GenBank· DDBJ | Genomic DNA |