G1UH28 · CHIT_PUNGR
- ProteinAcidic endochitinase Pun g 14, amyloplastic
- GenePSC
- StatusUniProtKB reviewed (Swiss-Prot)
- Organism
- Amino acids299 (go to sequence)
- Protein existenceEvidence at protein level
- Annotation score5/5
Function
function
Hydrolyzes chitin. Probable calcium storage protein of the seeds. Binds calcium ions with high capacity and low affinity. Involved in seed germination.
Catalytic activity
Activity regulation
Activity is not affected by addition of 10 mM Ca2+ or removal of Ca2+.
Kinetics
kcat/KM is 0.119 M-1sec-1 for native protein, 0.130 M-1sec-1 for native protein with 10 mM Ca2+ and 0.116 M-1sec-1 for EDTA-treated protein, using 4-methylumbelliferyl-beta-D-N,N',N''-triacetylchitotrioside (4-MU(GlcNAc)3) as substrate at pH 4.5 and 37 degrees Celsius.
pH Dependence
Optimum pH is between 2.5 and 5.0. Removal of Ca2+ from the protein by EDTA treatment narrows the pH range to 2.5-3.5.
Temperature Dependence
Optimum temperature is approximately 45 degrees Celsius. Removal of Ca2+ from the protein by EDTA treatment decreases the optimum temperature to 35 degrees Celsius.
Features
Showing features for active site.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Active site | 153 | Proton donor | ||||
Sequence: E |
GO annotations
Aspect | Term | |
---|---|---|
Cellular Component | amyloplast | |
Molecular Function | calcium ion binding | |
Molecular Function | chitinase activity | |
Biological Process | chitin catabolic process | |
Biological Process | polysaccharide catabolic process | |
Biological Process | seed germination | |
Biological Process | seedling development |
Keywords
- Molecular function
- Biological process
- Ligand
Enzyme and pathway databases
Protein family/group databases
Names & Taxonomy
Protein names
- Recommended nameAcidic endochitinase Pun g 14, amyloplastic
- EC number
- Alternative names
- Allergen namePun g 14
Gene names
Organism names
- Organism
- Strain
- Taxonomic lineageEukaryota > Viridiplantae > Streptophyta > Embryophyta > Tracheophyta > Spermatophyta > Magnoliopsida > eudicotyledons > Gunneridae > Pentapetalae > rosids > malvids > Myrtales > Lythraceae > Punica
Accessions
- Primary accessionG1UH28
Proteomes
Subcellular Location
UniProt Annotation
GO Annotation
Note: Localizes to stroma in amyloplasts of the embryonic cells of the seed.
Keywords
- Cellular component
Phenotypes & Variants
Allergenic properties
Causes an allergic reaction in human. Binds to IgE in 19% of 357 patients allergic to pomegranate.
Keywords
- Disease
Protein family/group databases
PTM/Processing
Features
Showing features for transit peptide, chain, disulfide bond.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Transit peptide | 1-26 | Amyloplast | ||||
Sequence: MAKTLPFSRALLLSLSILLVARAISA | ||||||
Chain | PRO_5011206581 | 27-299 | Acidic endochitinase Pun g 14, amyloplastic | |||
Sequence: GDIAIYWGQNGGEGTLASTCDTGRYAYVIVSFVTTFGNFRAPVVNLAGHCDPAAGTCTGLSDEIRSCQGKDIKVLMSIGGGAGDYSLVSEADADNFADYLWNNFLGGQSSSRPLGDAVLDGIDFDIELGTTTFYDTLARALSSRSTQAAKVYLTAAPQCPHPDSHLDAALNTGLFDNVWIQFYNNPLAQCQYSSGNTNDILSSWNTWTSSTTAGKIFLGLPAAPEAAGSGYIPPDVLTGQILPQIKTSAKYGGVMLYSKFYDTTYSTTIKDQV | ||||||
Disulfide bond | 46↔93 | |||||
Sequence: CDTGRYAYVIVSFVTTFGNFRAPVVNLAGHCDPAAGTCTGLSDEIRSC | ||||||
Disulfide bond | 76↔83 | |||||
Sequence: CDPAAGTC | ||||||
Disulfide bond | 185↔216 | |||||
Sequence: CPHPDSHLDAALNTGLFDNVWIQFYNNPLAQC |
Keywords
- PTM
Expression
Tissue specificity
Highly expressed in seeds and to a lesser extent in the skin of the pomegranate fruit (at protein level). Not expressed in leaves or flesh of the fruit (at protein level).
Developmental stage
Expressed at all stages of seed germination. Expression decreases markedly from day 1 to day 15 after imbibition.
Interaction
Subunit
Monomer.
Structure
Family & Domains
Features
Showing features for domain.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Domain | 27-299 | GH18 | ||||
Sequence: GDIAIYWGQNGGEGTLASTCDTGRYAYVIVSFVTTFGNFRAPVVNLAGHCDPAAGTCTGLSDEIRSCQGKDIKVLMSIGGGAGDYSLVSEADADNFADYLWNNFLGGQSSSRPLGDAVLDGIDFDIELGTTTFYDTLARALSSRSTQAAKVYLTAAPQCPHPDSHLDAALNTGLFDNVWIQFYNNPLAQCQYSSGNTNDILSSWNTWTSSTTAGKIFLGLPAAPEAAGSGYIPPDVLTGQILPQIKTSAKYGGVMLYSKFYDTTYSTTIKDQV |
Sequence similarities
Belongs to the glycosyl hydrolase 18 family. Chitinase class III subfamily.
Keywords
- Domain
Family and domain databases
Sequence
- Sequence statusComplete
- Sequence processingThe displayed sequence is further processed into a mature form.
- Length299
- Mass (Da)31,747
- Last updated2011-11-16 v1
- ChecksumD5D28157AA92D0B1
Features
Showing features for sequence conflict.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Sequence conflict | 90 | in Ref. 1; AA sequence | ||||
Sequence: I → L | ||||||
Sequence conflict | 295 | in Ref. 1; AA sequence | ||||
Sequence: I → L |
Mass Spectrometry
Keywords
- Technical term