G1DG82 · G1DG82_CAPHI
- ProteinDNA polymerase
- GenePOLB
- StatusUniProtKB unreviewed (TrEMBL)
- Organism
- Amino acids141 (go to sequence)
- Protein existenceEvidence at transcript level
- Annotation score3/5
Function
function
DNA polymerase that functions in several pathways of DNA repair. Involved in base excision repair (BER) responsible for repair of lesions that give rise to abasic (AP) sites in DNA. Also contributes to DNA double-strand break repair by non-homologous end joining and homologous recombination. Has both template-dependent and template-independent (terminal transferase) DNA polymerase activities. Has also a 5'-deoxyribose-5-phosphate lyase (dRP lyase) activity.
Catalytic activity
- 2'-deoxyribonucleotide-(2'-deoxyribose 5'-phosphate)-2'-deoxyribonucleotide-DNA = a 3'-end 2'-deoxyribonucleotide-(2,3-dehydro-2,3-deoxyribose 5'-phosphate)-DNA + a 5'-end 5'-phospho-2'-deoxyribonucleoside-DNA + H+
- a 5'-end 2'-deoxyribose-2'-deoxyribonucleotide-DNA = (2E,4S)-4-hydroxypenten-2-al-5-phosphate + a 5'-end 5'-phospho-2'-deoxyribonucleoside-DNA + H+
Features
Showing features for active site.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Active site | 72 | Nucleophile; Schiff-base intermediate with DNA; for 5'-dRP lyase activity | ||||
Sequence: K |
GO annotations
Aspect | Term | |
---|---|---|
Cellular Component | cytoplasm | |
Cellular Component | nucleus | |
Molecular Function | class I DNA-(apurinic or apyrimidinic site) endonuclease activity | |
Molecular Function | DNA binding | |
Molecular Function | DNA-directed DNA polymerase activity | |
Molecular Function | metal ion binding | |
Biological Process | base-excision repair | |
Biological Process | double-strand break repair via nonhomologous end joining |
Keywords
- Molecular function
- Biological process
- Ligand
Enzyme and pathway databases
Names & Taxonomy
Protein names
- Recommended nameDNA polymerase
- EC number
Gene names
Organism names
- Organism
- Taxonomic lineageEukaryota > Metazoa > Chordata > Craniata > Vertebrata > Euteleostomi > Mammalia > Eutheria > Laurasiatheria > Artiodactyla > Ruminantia > Pecora > Bovidae > Caprinae > Capra
Accessions
- Primary accessionG1DG82
PTM/Processing
Keywords
- PTM
Structure
Family & Domains
Features
Showing features for domain.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Domain | 10-141 | DNA-directed DNA polymerase X | ||||
Sequence: TLNGGITDMLTELANCEKNVTQAIHKDNAYRKAASVIAKYPHKIKSGAEAKKLPGVGTKIAEKIDEFLATGKLRKLEKIRQDDTSSSINFLTRVSGIGPSAARKFVDEGIKTLEDLRKNEDKLNHHQRIGLK | ||||||
Domain | 57-76 | Helix-hairpin-helix DNA-binding motif class 1 | ||||
Sequence: AEAKKLPGVGTKIAEKIDEF | ||||||
Domain | 98-117 | Helix-hairpin-helix DNA-binding motif class 1 | ||||
Sequence: NFLTRVSGIGPSAARKFVDE |
Sequence similarities
Belongs to the DNA polymerase type-X family.
Family and domain databases
Sequence
- Sequence statusComplete
- Length141
- Mass (Da)15,624
- Last updated2011-10-19 v1
- Checksum29224B31D3C31BFA