G1DFN8 · G1DFN8_CAPHI

  • Protein
    DNA repair nuclease/redox regulator APEX1
  • Gene
    APEX1
  • Status
    UniProtKB unreviewed (TrEMBL)
  • Amino acids
  • Protein existence
    Evidence at transcript level
  • Annotation score
    4/5

Function

function

Initiates repair of AP sites in DNA by catalyzing hydrolytic incision of the phosphodiester backbone immediately adjacent to the damage, generating a single-strand break with 5'-deoxyribose phosphate and 3'-hydroxyl ends.

Catalytic activity

  • Exonucleolytic cleavage in the 3'- to 5'-direction to yield nucleoside 5'-phosphates.
    EC:3.1.11.2 (UniProtKB | ENZYME | Rhea)

Cofactor

Protein has several cofactor binding sites:
Mg2+ (UniProtKB | Rhea| CHEBI:18420 )

Mn2+ (UniProtKB | Rhea| CHEBI:29035 )

Note: Probably binds two magnesium or manganese ions per subunit.
Mn2+ (UniProtKB | Rhea| CHEBI:29035 )

Features

Showing features for binding site, active site, site.

131850100150200250300
TypeIDPosition(s)Description
Binding site68Mg2+ 1 (UniProtKB | ChEBI)
Binding site96Mg2+ 1 (UniProtKB | ChEBI)
Active site171
Active site210Proton donor/acceptor
Binding site210Mg2+ 2 (UniProtKB | ChEBI)
Binding site212Mg2+ 2 (UniProtKB | ChEBI)
Site212Transition state stabilizer
Site283Important for catalytic activity
Binding site308Mg2+ 1 (UniProtKB | ChEBI)
Active site309Proton acceptor
Binding site309Mg2+ 2 (UniProtKB | ChEBI)
Site309Interaction with DNA substrate

GO annotations

AspectTerm
Cellular Componentmitochondrion
Cellular Componentnucleus
Molecular FunctionDNA binding
Molecular FunctionDNA-(apurinic or apyrimidinic site) endonuclease activity
Molecular Functiondouble-stranded DNA 3'-5' DNA exonuclease activity
Molecular Functionendonuclease activity
Molecular Functionlyase activity
Molecular Functionmetal ion binding
Molecular Functionphosphoric diester hydrolase activity
Biological Processbase-excision repair
Biological ProcessDNA recombination

Keywords

Enzyme and pathway databases

Names & Taxonomy

Protein names

  • Recommended name
    DNA repair nuclease/redox regulator APEX1
  • EC number
  • Short names
    APEN
    ; REF-1
  • Alternative names
    • APEX nuclease
    • Apurinic-apyrimidinic endonuclease 1
    • Redox factor-1
  • Cleaved into 1 chains

Gene names

    • Name
      APEX1

Organism names

  • Taxonomic identifier
  • Organism
  • Taxonomic lineage
    Eukaryota > Metazoa > Chordata > Craniata > Vertebrata > Euteleostomi > Mammalia > Eutheria > Laurasiatheria > Artiodactyla > Ruminantia > Pecora > Bovidae > Caprinae > Capra

Accessions

  • Primary accession
    G1DFN8

Subcellular Location

Nucleus
Cytoplasm
Mitochondrion

Keywords

Family & Domains

Features

Showing features for compositional bias, region, domain.

TypeIDPosition(s)Description
Compositional bias1-41Basic and acidic residues
Region1-59Disordered
Domain81-309Endonuclease/exonuclease/phosphatase

Sequence similarities

Belongs to the DNA repair enzymes AP/ExoA family.

Family and domain databases

Sequence

  • Sequence status
    Complete
  • Length
    318
  • Mass (Da)
    35,563
  • Last updated
    2011-10-19 v1
  • Checksum
    068B150A8F26C8DB
MPKRGIKGAVVEDAEEPKTEPEARKRKGGAKKNEKEAVGEGAVLYEDPADQKTSPSGKSATLKICPWNVDGRRAWIKKKGVDWIKEEAPDILCLQETKCSENKLPVELQELSGLPHQYWSAPSDKEGYSGVGLLSRQCPLKVSYGIGEEEHDQEGRVIVAGYDAFVLVTAYVPNAGRGLVRLEYRQRWDEAFRKFLKGLASRKPLVLCGDLNVAHEESDVRNPKGNKKNAGFTPQERQGFGELLQAVPLTDSFRHLYPNTAYAYTFWTYMMNARSKNVGWRLDYFLLSQSLLPAVCDSKIRSKALGSDHCPITLYLAL

Features

Showing features for compositional bias.

TypeIDPosition(s)Description
Compositional bias1-41Basic and acidic residues

Sequence databases

Nucleotide SequenceProtein SequenceMolecule TypeStatus
JI861604
EMBL· GenBank· DDBJ
AEJ84092.1
EMBL· GenBank· DDBJ
mRNA

Similar Proteins

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