G0UQ28 · G0UQ28_TRYCI

Function

function

Bifunctional enzyme that catalyzes the epimerization of the S- and R-forms of NAD(P)HX and the dehydration of the S-form of NAD(P)HX at the expense of ADP, which is converted to AMP. This allows the repair of both epimers of NAD(P)HX, a damaged form of NAD(P)H that is a result of enzymatic or heat-dependent hydration.
Catalyzes the dehydration of the S-form of NAD(P)HX at the expense of ATP, which is converted to ADP. Together with NAD(P)HX epimerase, which catalyzes the epimerization of the S- and R-forms, the enzyme allows the repair of both epimers of NAD(P)HX, a damaged form of NAD(P)H that is a result of enzymatic or heat-dependent hydration.
Catalyzes the epimerization of the S- and R-forms of NAD(P)HX, a damaged form of NAD(P)H that is a result of enzymatic or heat-dependent hydration. This is a prerequisite for the S-specific NAD(P)H-hydrate dehydratase to allow the repair of both epimers of NAD(P)HX.

Catalytic activity

Cofactor

Protein has several cofactor binding sites:
K+ (UniProtKB | Rhea| CHEBI:29103 )

Note: Binds 1 potassium ion per subunit.
Mg2+ (UniProtKB | Rhea| CHEBI:18420 )

Features

Showing features for binding site.

TypeIDPosition(s)Description
Binding site83-87(6S)-NADPHX (UniProtKB | ChEBI)
Binding site84K+ (UniProtKB | ChEBI)
Binding site155K+ (UniProtKB | ChEBI)
Binding site159-165(6S)-NADPHX (UniProtKB | ChEBI)
Binding site170(6S)-NADPHX (UniProtKB | ChEBI)
Binding site188(6S)-NADPHX (UniProtKB | ChEBI)
Binding site191K+ (UniProtKB | ChEBI)
Binding site355(6S)-NADPHX (UniProtKB | ChEBI)
Binding site424-430(6S)-NADPHX (UniProtKB | ChEBI)
Binding site461-465ATP (UniProtKB | ChEBI)
Binding site497-506ATP (UniProtKB | ChEBI)
Binding site507(6S)-NADPHX (UniProtKB | ChEBI)

GO annotations

AspectTerm
Molecular FunctionADP-dependent NAD(P)H-hydrate dehydratase activity
Molecular FunctionATP binding
Molecular FunctionATP-dependent NAD(P)H-hydrate dehydratase activity
Molecular Functionmetal ion binding
Molecular FunctionNAD(P)HX epimerase activity
Biological Processmetabolite repair
Biological Processnicotinamide nucleotide metabolic process

Keywords

Names & Taxonomy

Protein names

  • Recommended name
    Bifunctional NAD(P)H-hydrate repair enzyme
  • Alternative names
    • Nicotinamide nucleotide repair protein

Including 2 domains:

  • Recommended name
    ADP-dependent (S)-NAD(P)H-hydrate dehydratase
  • EC number
  • Alternative names
    • ADP-dependent NAD(P)HX dehydratase
  • Recommended name
    NAD(P)H-hydrate epimerase
  • EC number

Gene names

    • ORF names
      TCIL3000_7_3030

Organism names

  • Taxonomic identifier
  • Strain
    • IL3000
  • Taxonomic lineage
    Eukaryota > Discoba > Euglenozoa > Kinetoplastea > Metakinetoplastina > Trypanosomatida > Trypanosomatidae > Trypanosoma > Nannomonas

Accessions

  • Primary accession
    G0UQ28

Organism-specific databases

PTM/Processing

Keywords

Family & Domains

Features

Showing features for domain.

TypeIDPosition(s)Description
Domain35-245YjeF N-terminal
Domain260-567YjeF C-terminal

Sequence similarities

Belongs to the NnrD/CARKD family.
Belongs to the NnrE/AIBP family.
In the C-terminal section; belongs to the NnrD/CARKD family.
In the N-terminal section; belongs to the NnrE/AIBP family.

Family and domain databases

Sequence

  • Sequence status
    Complete
  • Length
    576
  • Mass (Da)
    61,106
  • Last updated
    2011-10-19 v1
  • MD5 Checksum
    B9BA7784D5AFCCB8D49D183B9E30815A
MSHITKSFLRHVGTTADCTEKQQSLLSSVWSAAWLRRAEVEAARAQGVALYALMQRAAAAAFSVFRREYPHTRHWLILCGSGNNGGDGFEIARLASEQVRVTVVAVEASKSLPIEATTAHAALLERIQQGAEVVMKDSRAWMEDTVLGDVDLVVDGLLGIGMGGPPRDHYAELIERVNKLPQPRVSIDIPSGLNAETGDVKGACVRADHTVTFIAIKPGLLTGLARNYVGKLHYDPLQLADWMLAPEQEQSIFCRRLESSHLVPLLSRSVPSSCIHKGANGKVLLVGGDSGFGGAIAMSAEAALRTGSGLVRVLTRAEHIAPLLCRCPEVMTETLTETSLRLALEWATCVAVGPGLGQAEWGKRALCLVDQHLRKSGIPSVWDADALNILAACNGWRDQGTCGVEDSTLVVSDKRLANRIITPHSGEAARLLGCTVAEVEQDRYHAAEELAAMYGGVVLLKGPGTVLYASEDMRATRLPPAAELLLSSARAVADVGNSGMATGGMGDVLTGIIAGFLAQRLSPWHAACVGCLVHGTAADLAATAQGGERGLTATALFDHLPACINPTPELFKHASL

Sequence databases

Nucleotide SequenceProtein SequenceMolecule TypeStatus
HE575320
EMBL· GenBank· DDBJ
CCC91489.1
EMBL· GenBank· DDBJ
Genomic DNA

Similar Proteins

Disclaimer

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