G0UJG8 · G0UJG8_TRYCI
- ProteinMannosyltransferase
- StatusUniProtKB unreviewed (TrEMBL)
- Amino acids742 (go to sequence)
- Protein existenceInferred from homology
- Annotation score2/5
Function
function
Mannosyltransferase that operates in the biosynthetic pathway of dolichol-linked oligosaccharides, the glycan precursors employed in protein asparagine (N)-glycosylation. The assembly of dolichol-linked oligosaccharides begins on the cytosolic side of the endoplasmic reticulum membrane and finishes in its lumen. The sequential addition of sugars to dolichol pyrophosphate produces dolichol-linked oligosaccharides containing fourteen sugars, including two GlcNAcs, nine mannoses and three glucoses. Once assembled, the oligosaccharide is transferred from the lipid to nascent proteins by oligosaccharyltransferases. In the lumen of the endoplasmic reticulum, adds the eighth mannose residue in an alpha-1,6 linkage onto Man7GlcNAc2-PP-dolichol to produce Man8GlcNAc2-PP-dolichol.
Catalytic activity
- an alpha-D-Man-(1->2)-alpha-D-Man-(1->2)-alpha-D-Man-(1->3)-[alpha-D-Man-(1->2)-alpha-D-Man-(1->3)-alpha-D-Man-(1->6)]-beta-D-Man-(1->4)-beta-D-GlcNAc-(1->4)-alpha-D-GlcNAc-diphospho-di-trans,poly-cis-dolichol + a di-trans,poly-cis-dolichyl beta-D-mannosyl phosphate = an alpha-D-Man-(1->2)-alpha-D-Man-(1->2)-alpha-D-Man-(1->3)-[alpha-D-Man-(1->2)-alpha-D-Man-(1->3)-[alpha-D-Man-(1->6)]-alpha-D-Man-(1->6)]-beta-D-Man-(1->4)-beta-D-GlcNAc-(1->4)-alpha-D-GlcNAc-diphospho-di-trans,poly-cis-dolichol + a di-trans,poly-cis-dolichyl phosphate + H+This reaction proceeds in the forward direction.
an α-D-Man-(1→2)-α-D-Man-(1→2)-α-D-Man-(1→3)-[α-D-Man-(1→2)-α-D-Man-(1→3)-α-D-Man-(1→6)]-β-D-Man-(1→4)-β-D-GlcNAc-(1→4)-α-D-GlcNAc-diphospho-di-trans,poly-cis-dolichol RHEA-COMP:19518 + a di-trans,poly-cis-dolichyl β-D-mannosyl phosphate RHEA-COMP:19501 = an α-D-Man-(1→2)-α-D-Man-(1→2)-α-D-Man-(1→3)-[α-D-Man-(1→2)-α-D-Man-(1→3)-[α-D-Man-(1→6)]-α-D-Man-(1→6)]-β-D-Man-(1→4)-β-D-GlcNAc-(1→4)-α-D-GlcNAc-diphospho-di-trans,poly-cis-dolichol RHEA-COMP:19519 + a di-trans,poly-cis-dolichyl phosphate RHEA-COMP:19498 + CHEBI:15378
Pathway
Protein modification; protein glycosylation.
GO annotations
Aspect | Term | |
---|---|---|
Cellular Component | endoplasmic reticulum membrane | |
Molecular Function | dolichyl-P-Man:Man(7)GlcNAc(2)-PP-dolichol alpha-1,6-mannosyltransferase | |
Biological Process | protein N-linked glycosylation |
Keywords
- Molecular function
Enzyme and pathway databases
Names & Taxonomy
Protein names
- Recommended nameMannosyltransferase
- EC number
Gene names
Organism names
- Strain
- Taxonomic lineageEukaryota > Discoba > Euglenozoa > Kinetoplastea > Metakinetoplastina > Trypanosomatida > Trypanosomatidae > Trypanosoma > Nannomonas
Accessions
- Primary accessionG0UJG8
Organism-specific databases
Subcellular Location
UniProt Annotation
GO Annotation
Endoplasmic reticulum membrane ; Multi-pass membrane protein
Features
Showing features for transmembrane.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Transmembrane | 62-82 | Helical | ||||
Sequence: FVGPWFIAVTVMIPFTFFSSY | ||||||
Transmembrane | 130-150 | Helical | ||||
Sequence: ASRIALGSLSCLSFWYVATGI | ||||||
Transmembrane | 162-183 | Helical | ||||
Sequence: IGSLVIVPATGRCSIAATNFYL | ||||||
Transmembrane | 203-220 | Helical | ||||
Sequence: FGMILCSFACGCSCRGMY | ||||||
Transmembrane | 232-251 | Helical | ||||
Sequence: ALFRCDTLVLLGPYALFVLV | ||||||
Transmembrane | 257-276 | Helical | ||||
Sequence: FLRGIVVGMASVIAVVIFSV | ||||||
Transmembrane | 312-339 | Helical | ||||
Sequence: YWYFLVALPRAFLLLYPLLIVLICMSWW | ||||||
Transmembrane | 381-398 | Helical | ||||
Sequence: YSILLVPSSLFVALYSAL | ||||||
Transmembrane | 405-424 | Helical | ||||
Sequence: FIMIVFPWFLVPLAAAGARL | ||||||
Transmembrane | 468-496 | Helical | ||||
Sequence: FGMWSTGLVIVLVLLYTGHLATVGFSVYL |
Keywords
- Cellular component
PTM/Processing
Features
Showing features for signal, chain.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Signal | 1-21 | |||||
Sequence: MFLGSVLCFSALLLCIFVVEL | ||||||
Chain | PRO_5003410493 | 22-742 | Mannosyltransferase | |||
Sequence: VCPYTKVEESFNMQAIHDFLLCRGVDCFDHLRSPGVVPRTFVGPWFIAVTVMIPFTFFSSYIVQLVSFFSYCAGYLLTDALQIVVSNGFGGVSSMEEFFALNPMLLSHASRIALGSLSCLSFWYVATGIDRLPGLKLSKGIGSLVIVPATGRCSIAATNFYLLVLLQFHLVFYASRPLPNTFGMILCSFACGCSCRGMYYKAIALLSAAAALFRCDTLVLLGPYALFVLVEGKVTFLRGIVVGMASVIAVVIFSVGMDSYLWGRLVWPEAVVLFFNTVENQSWKWGRQPAYWYFLVALPRAFLLLYPLLIVLICMSWWRVCSTIWSGGNRESAGNINSDLPHCSPLTRVFQVLMDVSEEYSILLVPSSLFVALYSALPHKEMRFIMIVFPWFLVPLAAAGARLCGEFLHESTQGRGGPNHKKESGWEKKGESNEKSRKGQFKGDAGFGMWSTGLVIVLVLLYTGHLATVGFSVYLSGDNYPGAVSLKRLQGTIEADLDNTTSCLNRRLARGGASNRSVSIFVDAFAAMSGINRFQKVHEVPLNSPRKSTAEKAESLLANSGRLTLLLAFPFRALFSVVNSVSAGNSLLQNEDLIHVDAAAGTSLGTIRNLRMAEKGFPTLSLTMQSRGLKMRYIKEETRFDEVTGLYNHEGVDYLIVRYSQRDLHRKHGKFDELFVTRMSNALPRLKYWVYRLLHLKGKNTGADSRDVEGEPFLIALGPRC |
Structure
Family & Domains
Features
Showing features for region, compositional bias.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Region | 433-461 | Disordered | ||||
Sequence: TQGRGGPNHKKESGWEKKGESNEKSRKGQ | ||||||
Compositional bias | 436-461 | Basic and acidic residues | ||||
Sequence: RGGPNHKKESGWEKKGESNEKSRKGQ |
Sequence similarities
Belongs to the glycosyltransferase 22 family.
Keywords
- Domain
Phylogenomic databases
Family and domain databases
Sequence
- Sequence statusComplete
- Length742
- Mass (Da)82,252
- Last updated2011-10-19 v1
- Checksum6E79D50EF7EFC8C9
Features
Showing features for compositional bias.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Compositional bias | 436-461 | Basic and acidic residues | ||||
Sequence: RGGPNHKKESGWEKKGESNEKSRKGQ |