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G0SAN7 · DUO1_CHATD

Function

function

Component of the DASH complex that connects microtubules with kinetochores and couples microtubule depolymerisation to chromosome movement; it is involved in retrieving kinetochores to the spindle poles before their re-orientation on the spindle in early mitosis and allows microtubule depolymerization to pull chromosomes apart and resist detachment during anaphase. Kinetochores, consisting of a centromere-associated inner segment and a microtubule-contacting outer segment, play a crucial role in chromosome segregation by mediating the physical connection between centromeric DNA and microtubules. Kinetochores also serve as an input point for the spindle assembly checkpoint, which delays anaphase until all chromosomes have bioriented on the mitotic spindle.

GO annotations

all annotationsall molecular functionvirus receptor activitydna bindingrna bindingcytoskeletal motor activitycatalytic activitygtpase activitystructural molecule activitytransporter activitycytoskeletal protein bindinglipid bindingcyclase activityantioxidant activityoxidoreductase activitytransferase activityhydrolase activitylyase activityisomerase activityligase activityprotein tag activitycargo receptor activityhistone bindingprotein folding chaperonetranslation regulator activitynutrient reservoir activityreceptor ligand activitymolecular transducer activitymolecular adaptor activitytoxin activitycell adhesion mediator activitymolecular function regulator activityvirus coreceptor activitycatalytic activity, acting on a proteincatalytic activity, acting on dnacatalytic activity, acting on rnamolecular carrier activitytranscription regulator activitygeneral transcription initiation factor activitymolecular sensor activitymolecular sequestering activityatp-dependent activityother molecular functionall biological processmitotic cell cyclecytokinesiscytoplasmic translationimmune system processmuscle system processcirculatory system processrenal system processrespiratory system processcarbohydrate metabolic processgeneration of precursor metabolites and energydna replicationdna repairdna recombinationchromatin organizationdna-templated transcriptionregulation of dna-templated transcriptiontrna metabolic processprotein foldingprotein glycosylationamino acid metabolic processmodified amino acid metabolic processlipid metabolic processvitamin metabolic processsulfur compound metabolic processintracellular protein transportnucleocytoplasmic transportautophagyinflammatory responsemitochondrion organizationcytoskeleton organizationmicrotubule-based movementperoxisome organizationlysosome organizationchromosome segregationcell adhesionestablishment or maintenance of cell polarityprogrammed cell deathphotosynthesismrna metabolic processsnrna metabolic processvesicle-mediated transportreproductive processdigestive system processsignalingcell differentiationprotein catabolic processextracellular matrix organizationregulatory ncrna-mediated gene silencingtelomere organizationcell junction organizationwound healingribosome biogenesiscilium organizationanatomical structure developmentcell motilitynervous system processendocrine processprotein maturationtransmembrane transportnucleobase-containing small molecule metabolic processhepaticobiliary system processmembrane organizationprotein-containing complex assemblycell wall organization or biogenesisnitrogen cycle metabolic processprotein localization to plasma membranedefense response to other organismdetoxificationmeiotic nuclear divisionmitotic nuclear divisionmitochondrial gene expressioncarbohydrate derivative metabolic processother biological processall cellular componentnuclear chromosomeextracellular regionextracellular spacecell wallnucleusnuclear envelopenucleoplasmchromosomenucleolusmitochondrionlysosomeendosomevacuoleperoxisomeendoplasmic reticulumgolgi apparatuslipid dropletmicrotubule organizing centercytosolribosomecytoskeletonplasma membraneciliumplastidthylakoidexternal encapsulating structureextracellular matrixcytoplasmic vesicleorganelleother cellular component
Cell color indicative of number of GO terms
AspectTerm
Cellular Componentcytoplasm
Cellular ComponentDASH complex
Cellular Componentkinetochore
Cellular Componentmicrotubule
Cellular Componentmitotic spindle
Cellular Componentspindle pole
Biological Processattachment of mitotic spindle microtubules to kinetochore
Biological Processattachment of spindle microtubules to kinetochore
Biological Processcell division
Biological Processmitotic sister chromatid biorientation
Biological Processprotein transport along microtubule to mitotic spindle pole body

Keywords

Names & Taxonomy

Protein names

  • Recommended name
    DASH complex subunit DUO1
  • Alternative names
    • Outer kinetochore protein DUO1

Gene names

    • Name
      DUO1
    • ORF names
      CTHT_0042930

Organism names

Accessions

  • Primary accession
    G0SAN7

Proteomes

PTM/Processing

Features

Showing features for chain.

TypeIDPosition(s)Description
ChainPRO_00004594631-232DASH complex subunit DUO1

Interaction

Subunit

Component of the DASH complex consisting of ASK1, DAD1, DAD2, DAD3, DAD4, DAM1, DUO1, HSK3, SPC19 and SPC34, with a stoichiometry of one copy of each subunit per complex (PubMed:29724956).
Multiple DASH complexes oligomerize to form a ring that encircles spindle microtubules and organizes the rod-like NDC80 complexes of the outer kinetochore (PubMed:29724956).
DASH complex oligomerization strengthens microtubule attachments (By similarity).
On cytoplasmic microtubules, DASH complexes appear to form patches instead of rings (By similarity).

Protein-protein interaction databases

Family & Domains

Features

Showing features for compositional bias, region, coiled coil.

Type
IDPosition(s)Description
Compositional bias1-18Basic and acidic residues
Region1-44Disordered
Compositional bias20-34Polar residues
Coiled coil128-171
Compositional bias133-167Basic and acidic residues
Region133-232Disordered
Compositional bias194-215Polar residues

Sequence similarities

Belongs to the DASH complex DUO1 family.

Keywords

Phylogenomic databases

Family and domain databases

Sequence

  • Sequence status
    Complete
  • Length
    232
  • Mass (Da)
    25,297
  • Last updated
    2011-10-19 v1
  • MD5 Checksum
    9BE530CE72C32EE5134AF479F458121C
MADEMDFYSSDYHNDDAFETPSKPPTSTSGRPKGSGAQGGGGMRFDTEEAREAALRKELEGVRKINEVIEGMIGTLERAKGNMGTVSQTVTNATTLLNTWTRMLSQTEHNQRLILNPEWKGATQDLLELEAEERRRQEEVERRAAEAERRREEARRKAEEEERRRAAAAAAAAAPAGRSVGRGTTRGRVRGSGLTRGASSSASGSETTRTTSGIARGGFGYTRGTARYRGAK

Features

Showing features for compositional bias.

TypeIDPosition(s)Description
Compositional bias1-18Basic and acidic residues
Compositional bias20-34Polar residues
Compositional bias133-167Basic and acidic residues
Compositional bias194-215Polar residues

Keywords

Sequence databases

Nucleotide SequenceProtein SequenceMolecule TypeStatus
GL988043
EMBL· GenBank· DDBJ
EGS19809.1
EMBL· GenBank· DDBJ
Genomic DNA

Genome annotation databases

Similar Proteins

Disclaimer

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