G0LET5 · BOA7_BOTFB

Function

function

Cytochrome P450 monooxygenase; part of the gene cluster B that mediates the biosynthesis of botcinic acid and its botcinin derivatives, acetate-derived polyketides that contribute to virulence when combined with the sesquiterpene botrydial (PubMed:21722295).
Botcinic acid and its derivatives have been shown to induce chlorosis and necrosis during host plant infection, but also have antifungal activities (PubMed:21722295).
Two polyketide synthases, BOA6 and BOA9, are involved in the biosynthesis of botcinins. BOA6 mediates the formation of the per-methylated tetraketide core by condensation of four units of malonyl-CoA with one unit of acetyl-CoA, which would be methylated in activated methylene groups to yield a bicyclic acid intermediate that could then either be converted to botrylactone derivatives or lose the starter acetate unit through a retro-Claisen type C-C bond cleavage to yield botcinin derivatives (PubMed:23203902).
The second polyketide synthase, BOA9, is probably required for the biosynthesis of the tetraketide side chain of botcinins (Probable). The methyltransferase (MT) domain within BOA6 is probably responsible for the incorporation of four methyl groups (Probable). The trans-enoyl reductase BOA5 might take over the enoyl reductase function of BOA6 that misses an ER domain (Probable). The monooxygenases BOA2, BOA3 and BOA4 might be involved in further hydroxylations at C4, C5 and C8, whereas BOA7, close to BOA9, could potentially be involved in the hydroxylation at C4 in the side chain of botcinins (Probable)

Cofactor

heme (UniProtKB | Rhea| CHEBI:30413 )

Pathway

Polyketide biosynthesis.

Features

Showing features for binding site.

TypeIDPosition(s)Description
Binding site478Fe (UniProtKB | ChEBI) of heme (UniProtKB | ChEBI); axial binding residue

GO annotations

AspectTerm
Cellular Componentmembrane
Molecular Functionheme binding
Molecular Functioniron ion binding
Molecular Functionmonooxygenase activity
Molecular Functionoxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen

Keywords

Enzyme and pathway databases

Names & Taxonomy

Protein names

  • Recommended name
    Cytochrome P450 monooxygenase BOA7
  • EC number
  • Alternative names
    • Botcinic acid biosynthesis cluster B protein 7

Gene names

    • Name
      BOA7

Organism names

Accessions

  • Primary accession
    G0LET5

Organism-specific databases

Subcellular Location

Membrane
; Single-pass membrane protein

Features

Showing features for transmembrane.

TypeIDPosition(s)Description
Transmembrane20-37Helical

Keywords

PTM/Processing

Features

Showing features for chain, glycosylation.

Type
IDPosition(s)Description
ChainPRO_00004446411-535Cytochrome P450 monooxygenase BOA7
Glycosylation65N-linked (GlcNAc...) asparagine
Glycosylation148N-linked (GlcNAc...) asparagine
Glycosylation180N-linked (GlcNAc...) asparagine
Glycosylation420N-linked (GlcNAc...) asparagine

Keywords

PTM databases

Expression

Induction

Expression of the botcinic acid clusters genes BOA1-13 and BOA17 is coregulated by BCG1 during both in vitro and in planta growth.

Structure

Family & Domains

Sequence similarities

Belongs to the cytochrome P450 family.

Keywords

Phylogenomic databases

Family and domain databases

Sequence

  • Sequence status
    Complete
  • Length
    535
  • Mass (Da)
    60,666
  • Last updated
    2011-10-19 v1
  • Checksum
    37E6C15C38083B59
MYQRLKEINFRKPLELEWNSIFLILGFFVLAAILIAWTQRPDEELLLERLKDLDLPIVGVGPNINVSESLEIGTQTYPNSPYVVPADRVPIVILPNSAINIIKSLPEAKISFEKEVYARHLAHLLLKKDQKIFSEPILNSIKQDLTRNISKTLDTLWDEVDYAFEKNIGTLPDDDDGWKNVSVYGKVLNVVALLSGRVFVGAPLCRDEEWIKATIAYTIILGVTVGMLWKRPWWQRKILAPLYFRTLVGVHKKAEELLKPLLEREAALDPSEWEKKAGEQNDGQLIRWLLSHTPQKGGKIDVKQLAHDQLTVSLAAIHTTSITISHLLYDLATYPEHVAPLRTELESVIADHKTAGGNGKLSKVELTKLWKMDSFIKESQRLNPPILVQMRRYLTSPLALPSGHILPSGTFCGVDAQMTNRTVPYYEASPITHQQTPFDTFDGFRFSKLRSVPGNENRYQFVTSSTESLNFGHGTHACPGRFFASNEIKIAFAEVLLKWDVRLKPGEGRPANLYTDTNVMPNMKGEVQMRRRELI

Sequence databases

Nucleotide SequenceProtein SequenceMolecule TypeStatus
FR717895
EMBL· GenBank· DDBJ
CBX55165.1
EMBL· GenBank· DDBJ
Genomic DNA

Genome annotation databases

Similar Proteins

Disclaimer

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