G0LET5 · BOA7_BOTFB
- ProteinCytochrome P450 monooxygenase BOA7
- GeneBOA7
- StatusUniProtKB reviewed (Swiss-Prot)
- Amino acids535 (go to sequence)
- Protein existenceEvidence at transcript level
- Annotation score3/5
Function
function
Cytochrome P450 monooxygenase; part of the gene cluster B that mediates the biosynthesis of botcinic acid and its botcinin derivatives, acetate-derived polyketides that contribute to virulence when combined with the sesquiterpene botrydial (PubMed:21722295).
Botcinic acid and its derivatives have been shown to induce chlorosis and necrosis during host plant infection, but also have antifungal activities (PubMed:21722295).
Two polyketide synthases, BOA6 and BOA9, are involved in the biosynthesis of botcinins. BOA6 mediates the formation of the per-methylated tetraketide core by condensation of four units of malonyl-CoA with one unit of acetyl-CoA, which would be methylated in activated methylene groups to yield a bicyclic acid intermediate that could then either be converted to botrylactone derivatives or lose the starter acetate unit through a retro-Claisen type C-C bond cleavage to yield botcinin derivatives (PubMed:23203902).
The second polyketide synthase, BOA9, is probably required for the biosynthesis of the tetraketide side chain of botcinins (Probable). The methyltransferase (MT) domain within BOA6 is probably responsible for the incorporation of four methyl groups (Probable). The trans-enoyl reductase BOA5 might take over the enoyl reductase function of BOA6 that misses an ER domain (Probable). The monooxygenases BOA2, BOA3 and BOA4 might be involved in further hydroxylations at C4, C5 and C8, whereas BOA7, close to BOA9, could potentially be involved in the hydroxylation at C4 in the side chain of botcinins (Probable)
Botcinic acid and its derivatives have been shown to induce chlorosis and necrosis during host plant infection, but also have antifungal activities (PubMed:21722295).
Two polyketide synthases, BOA6 and BOA9, are involved in the biosynthesis of botcinins. BOA6 mediates the formation of the per-methylated tetraketide core by condensation of four units of malonyl-CoA with one unit of acetyl-CoA, which would be methylated in activated methylene groups to yield a bicyclic acid intermediate that could then either be converted to botrylactone derivatives or lose the starter acetate unit through a retro-Claisen type C-C bond cleavage to yield botcinin derivatives (PubMed:23203902).
The second polyketide synthase, BOA9, is probably required for the biosynthesis of the tetraketide side chain of botcinins (Probable). The methyltransferase (MT) domain within BOA6 is probably responsible for the incorporation of four methyl groups (Probable). The trans-enoyl reductase BOA5 might take over the enoyl reductase function of BOA6 that misses an ER domain (Probable). The monooxygenases BOA2, BOA3 and BOA4 might be involved in further hydroxylations at C4, C5 and C8, whereas BOA7, close to BOA9, could potentially be involved in the hydroxylation at C4 in the side chain of botcinins (Probable)
Cofactor
Pathway
Polyketide biosynthesis.
Features
Showing features for binding site.
Type | ID | Position(s) | Description | ||
---|---|---|---|---|---|
Binding site | 478 | Fe (UniProtKB | ChEBI) of heme (UniProtKB | ChEBI); axial binding residue | |||
GO annotations
Aspect | Term | |
---|---|---|
Cellular Component | membrane | |
Molecular Function | heme binding | |
Molecular Function | iron ion binding | |
Molecular Function | monooxygenase activity | |
Molecular Function | oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen |
Keywords
- Molecular function
- Biological process
- Ligand
Enzyme and pathway databases
Names & Taxonomy
Protein names
- Recommended nameCytochrome P450 monooxygenase BOA7
- EC number
- Alternative names
Gene names
Organism names
- Strain
- Taxonomic lineageEukaryota > Fungi > Dikarya > Ascomycota > Pezizomycotina > Leotiomycetes > Helotiales > Sclerotiniaceae > Botrytis
Accessions
- Primary accessionG0LET5
Organism-specific databases
Subcellular Location
UniProt Annotation
GO Annotation
Membrane ; Single-pass membrane protein
Features
Showing features for transmembrane.
Type | ID | Position(s) | Description | ||
---|---|---|---|---|---|
Transmembrane | 20-37 | Helical | |||
Keywords
- Cellular component
PTM/Processing
Features
Showing features for chain, glycosylation.
Type | ID | Position(s) | Description | ||
---|---|---|---|---|---|
Chain | PRO_0000444641 | 1-535 | Cytochrome P450 monooxygenase BOA7 | ||
Glycosylation | 65 | N-linked (GlcNAc...) asparagine | |||
Glycosylation | 148 | N-linked (GlcNAc...) asparagine | |||
Glycosylation | 180 | N-linked (GlcNAc...) asparagine | |||
Glycosylation | 420 | N-linked (GlcNAc...) asparagine | |||
Keywords
- PTM
PTM databases
Expression
Induction
Expression of the botcinic acid clusters genes BOA1-13 and BOA17 is coregulated by BCG1 during both in vitro and in planta growth.
Structure
Sequence
- Sequence statusComplete
- Length535
- Mass (Da)60,666
- Last updated2011-10-19 v1
- Checksum37E6C15C38083B59
Sequence databases
Nucleotide Sequence | Protein Sequence | Molecule Type | Status | |
---|---|---|---|---|
FR717895 EMBL· GenBank· DDBJ | CBX55165.1 EMBL· GenBank· DDBJ | Genomic DNA |