F9X9V1 · NRPS2_ZYMTI
- ProteinNonribosomal peptide synthetase 2
- GeneNRPS2
- StatusUniProtKB reviewed (Swiss-Prot)
- Amino acids4476 (go to sequence)
- Protein existenceInferred from homology
- Annotation score3/5
Function
function
Nonribosomal peptide synthetase; part of the gene cluster 14 that mediates the biosynthesis of a ferrichrome A-like siderophore which may contribute to organismal virulence (Probable). The first step of siderophore biosynthesis is performed by the HMG-CoA synthase (HMGS) MYCGRDRAFT_54740 which catalyzes the generation of HMG-CoA and CoA using acetoacetyl-CoA and acetyl-CoA as substrates (By similarity).
The enoyl-CoA isomerase/hydratase MYCGRDRAFT_76805 then catalyzes the conversion of HMG-CoA to methylglutaconyl-CoA (By similarity).
The acyltransferase MYCGRDRAFT_85486 then fuses methylglutaconyl-CoA with hydroxyornithine to yield methylglutaconyl hydroxyornithine (By similarity).
Methylglutaconyl hydroxyornithine is then available for use by the nonribosomal peptide synthetase NRPS2 to generate the ferrichrome A-like siderophore (By similarity).
The enoyl-CoA isomerase/hydratase MYCGRDRAFT_76805 then catalyzes the conversion of HMG-CoA to methylglutaconyl-CoA (By similarity).
The acyltransferase MYCGRDRAFT_85486 then fuses methylglutaconyl-CoA with hydroxyornithine to yield methylglutaconyl hydroxyornithine (By similarity).
Methylglutaconyl hydroxyornithine is then available for use by the nonribosomal peptide synthetase NRPS2 to generate the ferrichrome A-like siderophore (By similarity).
Pathway
Siderophore biosynthesis.
GO annotations
all annotations | all molecular function | virus receptor activity | dna binding | rna binding | cytoskeletal motor activity | catalytic activity | gtpase activity | structural molecule activity | transporter activity | cytoskeletal protein binding | lipid binding | cyclase activity | antioxidant activity | oxidoreductase activity | transferase activity | hydrolase activity | lyase activity | isomerase activity | ligase activity | protein tag activity | cargo receptor activity | histone binding | protein folding chaperone | translation regulator activity | nutrient reservoir activity | receptor ligand activity | molecular transducer activity | molecular adaptor activity | toxin activity | cell adhesion mediator activity | molecular function regulator activity | virus coreceptor activity | catalytic activity, acting on a protein | catalytic activity, acting on dna | catalytic activity, acting on rna | molecular carrier activity | transcription regulator activity | general transcription initiation factor activity | molecular sensor activity | molecular sequestering activity | atp-dependent activity | other molecular function | all biological process | mitotic cell cycle | cytokinesis | cytoplasmic translation | immune system process | muscle system process | circulatory system process | renal system process | respiratory system process | carbohydrate metabolic process | generation of precursor metabolites and energy | dna replication | dna repair | dna recombination | chromatin organization | dna-templated transcription | regulation of dna-templated transcription | trna metabolic process | protein folding | protein glycosylation | amino acid metabolic process | modified amino acid metabolic process | lipid metabolic process | vitamin metabolic process | sulfur compound metabolic process | intracellular protein transport | nucleocytoplasmic transport | autophagy | inflammatory response | mitochondrion organization | cytoskeleton organization | microtubule-based movement | peroxisome organization | lysosome organization | chromosome segregation | cell adhesion | establishment or maintenance of cell polarity | programmed cell death | photosynthesis | mrna metabolic process | snrna metabolic process | vesicle-mediated transport | reproductive process | digestive system process | signaling | cell differentiation | protein catabolic process | extracellular matrix organization | regulatory ncrna-mediated gene silencing | telomere organization | cell junction organization | wound healing | ribosome biogenesis | cilium organization | anatomical structure development | cell motility | nervous system process | endocrine process | protein maturation | transmembrane transport | nucleobase-containing small molecule metabolic process | hepaticobiliary system process | membrane organization | protein-containing complex assembly | cell wall organization or biogenesis | nitrogen cycle metabolic process | protein localization to plasma membrane | defense response to other organism | detoxification | meiotic nuclear division | mitotic nuclear division | mitochondrial gene expression | carbohydrate derivative metabolic process | other biological process | all cellular component | nuclear chromosome | extracellular region | extracellular space | cell wall | nucleus | nuclear envelope | nucleoplasm | chromosome | nucleolus | mitochondrion | lysosome | endosome | vacuole | peroxisome | endoplasmic reticulum | golgi apparatus | lipid droplet | microtubule organizing center | cytosol | ribosome | cytoskeleton | plasma membrane | cilium | plastid | thylakoid | external encapsulating structure | extracellular matrix | cytoplasmic vesicle | organelle | other cellular component | |||
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Aspect | Term | |
---|---|---|
Cellular Component | cytoplasm | |
Molecular Function | ligase activity | |
Molecular Function | phosphopantetheine binding | |
Biological Process | amino acid activation for nonribosomal peptide biosynthetic process | |
Biological Process | secondary metabolite biosynthetic process |
Keywords
- Molecular function
- Biological process
Enzyme and pathway databases
Names & Taxonomy
Protein names
- Recommended nameNonribosomal peptide synthetase 2
- EC number
- Alternative names
Gene names
Organism names
- Strain
- Taxonomic lineageEukaryota > Fungi > Dikarya > Ascomycota > Pezizomycotina > Dothideomycetes > Dothideomycetidae > Mycosphaerellales > Mycosphaerellaceae > Zymoseptoria
Accessions
- Primary accessionF9X9V1
Proteomes
Subcellular Location
UniProt Annotation
GO Annotation
PTM/Processing
Features
Showing features for chain, modified residue.
Type | ID | Position(s) | Description | ||
---|---|---|---|---|---|
Chain | PRO_0000451093 | 1-4476 | Nonribosomal peptide synthetase 2 | ||
Modified residue | 749 | O-(pantetheine 4'-phosphoryl)serine | |||
Modified residue | 1558 | O-(pantetheine 4'-phosphoryl)serine | |||
Modified residue | 2761 | O-(pantetheine 4'-phosphoryl)serine | |||
Modified residue | 3341 | O-(pantetheine 4'-phosphoryl)serine | |||
Modified residue | 3928 | O-(pantetheine 4'-phosphoryl)serine | |||
Keywords
- PTM
Interaction
Protein-protein interaction databases
Family & Domains
Features
Showing features for region, domain, compositional bias.
Type | ID | Position(s) | Description | ||
---|---|---|---|---|---|
Region | 156-587 | Adenylation 1 | |||
Domain | 714-790 | Carrier 1 | |||
Region | 826-1261 | Condensation 1 | |||
Domain | 1524-1598 | Carrier 2 | |||
Region | 1645-2106 | Condensation 2 | |||
Region | 2151-2585 | Adenylation 2 | |||
Domain | 2724-2800 | Carrier 3 | |||
Region | 2867-3232 | Condensation 3 | |||
Domain | 3307-3381 | Carrier 4 | |||
Region | 3418-3854 | Condensation 4 | |||
Compositional bias | 3858-3878 | Polar residues | |||
Region | 3858-3888 | Disordered | |||
Domain | 3889-3967 | Carrier 5 | |||
Region | 4030-4300 | Condensation 5 | |||
Domain
NRP synthetases are composed of discrete domains (adenylation (A), thiolation (T) or peptidyl carrier protein (PCP) and condensation (C) domains) which when grouped together are referred to as a single module. Each module is responsible for the recognition (via the A domain) and incorporation of a single amino acid into the growing peptide product. Thus, an NRP synthetase is generally composed of one or more modules and can terminate in a thioesterase domain (TE) that releases the newly synthesized peptide from the enzyme. Occasionally, epimerase (E) domains (responsible for L- to D- amino acid conversion) are present within the NRP synthetase. NRPS2 has the following architecture: A-T-C-T-C-A-T-C-T-C-T-C (Probable). The lack of corresponding A domains in the 3 modules suggests that A domains have to be used iteratively to incorporate the six amino acids in the siderophore (By similarity).
Sequence similarities
Belongs to the NRP synthetase family.
Keywords
- Domain
Phylogenomic databases
Family and domain databases
Sequence
- Sequence statusComplete
- Length4,476
- Mass (Da)492,986
- Last updated2011-10-19 v1
- MD5 Checksum8757674627DA6C16C5DBD7F88E02B482
Features
Showing features for compositional bias.
Type | ID | Position(s) | Description | ||
---|---|---|---|---|---|
Compositional bias | 3858-3878 | Polar residues | |||
Keywords
- Technical term
Sequence databases
Nucleotide Sequence | Protein Sequence | Molecule Type | Status | |
---|---|---|---|---|
CM001199 EMBL· GenBank· DDBJ | EGP88586.1 EMBL· GenBank· DDBJ | Genomic DNA |