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F9X9V1 · NRPS2_ZYMTI

Function

function

Nonribosomal peptide synthetase; part of the gene cluster 14 that mediates the biosynthesis of a ferrichrome A-like siderophore which may contribute to organismal virulence (Probable). The first step of siderophore biosynthesis is performed by the HMG-CoA synthase (HMGS) MYCGRDRAFT_54740 which catalyzes the generation of HMG-CoA and CoA using acetoacetyl-CoA and acetyl-CoA as substrates (By similarity).
The enoyl-CoA isomerase/hydratase MYCGRDRAFT_76805 then catalyzes the conversion of HMG-CoA to methylglutaconyl-CoA (By similarity).
The acyltransferase MYCGRDRAFT_85486 then fuses methylglutaconyl-CoA with hydroxyornithine to yield methylglutaconyl hydroxyornithine (By similarity).
Methylglutaconyl hydroxyornithine is then available for use by the nonribosomal peptide synthetase NRPS2 to generate the ferrichrome A-like siderophore (By similarity).

Pathway

Siderophore biosynthesis.

GO annotations

all annotationsall molecular functionvirus receptor activitydna bindingrna bindingcytoskeletal motor activitycatalytic activitygtpase activitystructural molecule activitytransporter activitycytoskeletal protein bindinglipid bindingcyclase activityantioxidant activityoxidoreductase activitytransferase activityhydrolase activitylyase activityisomerase activityligase activityprotein tag activitycargo receptor activityhistone bindingprotein folding chaperonetranslation regulator activitynutrient reservoir activityreceptor ligand activitymolecular transducer activitymolecular adaptor activitytoxin activitycell adhesion mediator activitymolecular function regulator activityvirus coreceptor activitycatalytic activity, acting on a proteincatalytic activity, acting on dnacatalytic activity, acting on rnamolecular carrier activitytranscription regulator activitygeneral transcription initiation factor activitymolecular sensor activitymolecular sequestering activityatp-dependent activityother molecular functionall biological processmitotic cell cyclecytokinesiscytoplasmic translationimmune system processmuscle system processcirculatory system processrenal system processrespiratory system processcarbohydrate metabolic processgeneration of precursor metabolites and energydna replicationdna repairdna recombinationchromatin organizationdna-templated transcriptionregulation of dna-templated transcriptiontrna metabolic processprotein foldingprotein glycosylationamino acid metabolic processmodified amino acid metabolic processlipid metabolic processvitamin metabolic processsulfur compound metabolic processintracellular protein transportnucleocytoplasmic transportautophagyinflammatory responsemitochondrion organizationcytoskeleton organizationmicrotubule-based movementperoxisome organizationlysosome organizationchromosome segregationcell adhesionestablishment or maintenance of cell polarityprogrammed cell deathphotosynthesismrna metabolic processsnrna metabolic processvesicle-mediated transportreproductive processdigestive system processsignalingcell differentiationprotein catabolic processextracellular matrix organizationregulatory ncrna-mediated gene silencingtelomere organizationcell junction organizationwound healingribosome biogenesiscilium organizationanatomical structure developmentcell motilitynervous system processendocrine processprotein maturationtransmembrane transportnucleobase-containing small molecule metabolic processhepaticobiliary system processmembrane organizationprotein-containing complex assemblycell wall organization or biogenesisnitrogen cycle metabolic processprotein localization to plasma membranedefense response to other organismdetoxificationmeiotic nuclear divisionmitotic nuclear divisionmitochondrial gene expressioncarbohydrate derivative metabolic processother biological processall cellular componentnuclear chromosomeextracellular regionextracellular spacecell wallnucleusnuclear envelopenucleoplasmchromosomenucleolusmitochondrionlysosomeendosomevacuoleperoxisomeendoplasmic reticulumgolgi apparatuslipid dropletmicrotubule organizing centercytosolribosomecytoskeletonplasma membraneciliumplastidthylakoidexternal encapsulating structureextracellular matrixcytoplasmic vesicleorganelleother cellular component
Cell color indicative of number of GO terms
AspectTerm
Cellular Componentcytoplasm
Molecular Functionligase activity
Molecular Functionphosphopantetheine binding
Biological Processamino acid activation for nonribosomal peptide biosynthetic process
Biological Processsecondary metabolite biosynthetic process

Keywords

Enzyme and pathway databases

Names & Taxonomy

Protein names

  • Recommended name
    Nonribosomal peptide synthetase 2
  • EC number
  • Alternative names
    • Ferrichrome A-like siderophore biosynthesis protein NRPS2

Gene names

    • Name
      NRPS2
    • ORF names
      MYCGRDRAFT_92222

Organism names

Accessions

  • Primary accession
    F9X9V1

Proteomes

Subcellular Location

PTM/Processing

Features

Showing features for chain, modified residue.

Type
IDPosition(s)Description
ChainPRO_00004510931-4476Nonribosomal peptide synthetase 2
Modified residue749O-(pantetheine 4'-phosphoryl)serine
Modified residue1558O-(pantetheine 4'-phosphoryl)serine
Modified residue2761O-(pantetheine 4'-phosphoryl)serine
Modified residue3341O-(pantetheine 4'-phosphoryl)serine
Modified residue3928O-(pantetheine 4'-phosphoryl)serine

Keywords

Interaction

Protein-protein interaction databases

Structure

3D structure databases

Family & Domains

Features

Showing features for region, domain, compositional bias.

Type
IDPosition(s)Description
Region156-587Adenylation 1
Domain714-790Carrier 1
Region826-1261Condensation 1
Domain1524-1598Carrier 2
Region1645-2106Condensation 2
Region2151-2585Adenylation 2
Domain2724-2800Carrier 3
Region2867-3232Condensation 3
Domain3307-3381Carrier 4
Region3418-3854Condensation 4
Compositional bias3858-3878Polar residues
Region3858-3888Disordered
Domain3889-3967Carrier 5
Region4030-4300Condensation 5

Domain

NRP synthetases are composed of discrete domains (adenylation (A), thiolation (T) or peptidyl carrier protein (PCP) and condensation (C) domains) which when grouped together are referred to as a single module. Each module is responsible for the recognition (via the A domain) and incorporation of a single amino acid into the growing peptide product. Thus, an NRP synthetase is generally composed of one or more modules and can terminate in a thioesterase domain (TE) that releases the newly synthesized peptide from the enzyme. Occasionally, epimerase (E) domains (responsible for L- to D- amino acid conversion) are present within the NRP synthetase. NRPS2 has the following architecture: A-T-C-T-C-A-T-C-T-C-T-C (Probable). The lack of corresponding A domains in the 3 modules suggests that A domains have to be used iteratively to incorporate the six amino acids in the siderophore (By similarity).

Sequence similarities

Belongs to the NRP synthetase family.

Keywords

Phylogenomic databases

Family and domain databases

Sequence

  • Sequence status
    Complete
  • Length
    4,476
  • Mass (Da)
    492,986
  • Last updated
    2011-10-19 v1
  • MD5 Checksum
    8757674627DA6C16C5DBD7F88E02B482

Features

Showing features for compositional bias.

TypeIDPosition(s)Description
Compositional bias3858-3878Polar residues

Keywords

Sequence databases

Nucleotide SequenceProtein SequenceMolecule TypeStatus
CM001199
EMBL· GenBank· DDBJ
EGP88586.1
EMBL· GenBank· DDBJ
Genomic DNA

Genome annotation databases

Similar Proteins

Disclaimer

Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care. Our staff consists of biologists and biochemists that are not trained to give medical advice.
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