F9UST4 · LARE_LACPL
- ProteinPyridinium-3,5-bisthiocarboxylic acid mononucleotide synthase
- GenelarE
- StatusUniProtKB reviewed (Swiss-Prot)
- Amino acids276 (go to sequence)
- Protein existenceEvidence at protein level
- Annotation score5/5
Function
function
Involved in the biosynthesis of a nickel-pincer cofactor ((SCS)Ni(II) pincer complex). Catalyzes the ATP-dependent incorporation of two sulfur atoms in pyridinium-3,5-biscarboxylic acid mononucleotide (P2CMN) to yield pyridinium-3,5-bisthiocarboxylic acid mononucleotide (P2TMN). The source of sulfur is the enzyme itself: Cys-176 of LarE is the sulfur donor, thereby being converted into dehydroalanine, and is not regenerated in vivo. Thus, two molecules of LarE undergo sacrificial sulfur transfer to create one P2TMN (PubMed:27114550).
Binds nickel (PubMed:24710389).
Is required for the activation of the lactate racemase LarA (PubMed:24710389).
May also be involved in the activation of other nickel-pincer cofactor-dependent enzymes (PubMed:27114550).
Binds nickel (PubMed:24710389).
Is required for the activation of the lactate racemase LarA (PubMed:24710389).
May also be involved in the activation of other nickel-pincer cofactor-dependent enzymes (PubMed:27114550).
Catalytic activity
- [LarE protein]-L-cysteine + ATP + pyridinium-3,5-dicarboxylate mononucleotide = [LarE protein]-dehydroalanine + AMP + diphosphate + H+ + pyridinium-3-carboxylate-5-thiocarboxylate mononucleotide
Features
Showing features for active site.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Active site | 176 | Nucleophile and sulfur donor | ||||
Sequence: C |
GO annotations
Aspect | Term | |
---|---|---|
Molecular Function | ATP binding | |
Molecular Function | lyase activity | |
Molecular Function | sulfurtransferase activity |
Keywords
- Molecular function
- Ligand
Enzyme and pathway databases
Names & Taxonomy
Protein names
- Recommended namePyridinium-3,5-bisthiocarboxylic acid mononucleotide synthase
- EC number
- Alternative names
Gene names
Organism names
- Strain
- Taxonomic lineageBacteria > Bacillota > Bacilli > Lactobacillales > Lactobacillaceae > Lactiplantibacillus
Accessions
- Primary accessionF9UST4
Proteomes
Phenotypes & Variants
Disruption phenotype
Deletion of this gene leads to a loss of lactate racemase activity.
Features
Showing features for mutagenesis.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Mutagenesis | 30 | Loss of activity. | ||||
Sequence: D → A | ||||||
Mutagenesis | 176 | Loss of activity. | ||||
Sequence: C → A |
PTM/Processing
Features
Showing features for chain, modified residue.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Chain | PRO_0000441655 | 1-276 | Pyridinium-3,5-bisthiocarboxylic acid mononucleotide synthase | |||
Sequence: MATLATKKATLVAALKDLQRVTVAFSGGIDSTLVLKMALDVLGRDNVTAVVANSELFTDEEFDKAMSLAEELGANVQGTTLDYLSDDHIKNNTPDSWYYAKKMFYSRLNDIAANNGSAAVLDGMIKNDENDYRPGLKARSEAGARSLLQEADFFKTDVRALAQELGLTNWNKVASCSVSSRFPYGTTLTHDNIAQVMAAEKYLRSLGFPTVRVRFHNDIARIELPEARIGDFLVFNDRVNRQLQSLGFRYVTLDLGGFRSGRMNDTLTKAQLATFA | ||||||
Modified residue | 176 | 2,3-didehydroalanine (Cys) | ||||
Sequence: C |
Expression
Induction
Induced by L-lactate and repressed by D-lactate. Is thus regulated by the L-lactate/D-lactate ratio. Makes part of the lar operon (larABCDE).
Interaction
Protein-protein interaction databases
Structure
Sequence
- Sequence statusComplete
- Length276
- Mass (Da)30,484
- Last updated2011-10-19 v1
- Checksum94B4C899BBAD22EE
Keywords
- Technical term
Sequence databases
Nucleotide Sequence | Protein Sequence | Molecule Type | Status | |
---|---|---|---|---|
AL935263 EMBL· GenBank· DDBJ | CCC77665.1 EMBL· GenBank· DDBJ | Genomic DNA |