F8WRK9 · CEEP_RHOMR
- ProteinCellobiose 2-epimerase
- Genece
- StatusUniProtKB reviewed (Swiss-Prot)
- Amino acids412 (go to sequence)
- Protein existenceEvidence at protein level
- Annotation score3/5
Function
function
Catalyzes the reversible epimerization of cellobiose to 4-O-beta-D-glucopyranosyl-D-mannose (Glc-Man). Can also use lactose, epilactose, mannobiose and cellotriose. Highly specific for oligosaccharides linked by the beta-1,4-glycosidic linkage. Shows preference for lactose.
Catalytic activity
- D-cellobiose = beta-D-glucosyl-(1->4)-D-mannopyranose
Kinetics
KM | SUBSTRATE | pH | TEMPERATURE[C] | NOTES | EVIDENCE | |
---|---|---|---|---|---|---|
27.2 mM | cellobiose | |||||
28.8 mM | lactose |
kcat is 80.8 sec-1 for cellobiose. kcat is 111 sec-1 for lactose.
pH Dependence
Optimum pH is 6.3.
Temperature Dependence
Optimum temperature is 80 degrees Celsius.
GO annotations
Aspect | Term | |
---|---|---|
Molecular Function | cellobiose epimerase activity | |
Molecular Function | N-acylglucosamine 2-epimerase activity | |
Biological Process | carbohydrate metabolic process | |
Biological Process | N-acetylglucosamine metabolic process | |
Biological Process | N-acetylmannosamine metabolic process |
Keywords
- Molecular function
Enzyme and pathway databases
Names & Taxonomy
Protein names
- Recommended nameCellobiose 2-epimerase
- EC number
- Short namesCE
Gene names
Organism names
- Strain
- Taxonomic lineageBacteria > Rhodothermota > Rhodothermia > Rhodothermales > Rhodothermaceae > Rhodothermus
Accessions
- Primary accessionF8WRK9
PTM/Processing
Features
Showing features for chain.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Chain | PRO_0000421447 | 1-412 | Cellobiose 2-epimerase | |||
Sequence: MSTETIPDVRRLRALQAEVHEELTENILKFWATRTHDPVHGGFVGRVGPDGRPHPEAPRGAILNARILWTFAAAYRQLGTPLYREMAERAYRYFVRHFVDAEHGGVYWMVAADGRPLDTRKHVYAQSFAIYALSEWHRATGGEAALALARSIYDLIETHCADRVHGGYVEACDRAWRPLEDARLSAKDAPEPRSMNTHLHVLEAYANLYRVWPETELAARLQALIELFLRAIYHPATGHLILFFDERWRPRSRAVSFGHDIEASWLLLEAVDVLGQATLRPRVQQASLHLARATLAEGRAPDGSLYYEIGEQGHLDTDRHWWPQAEALVGFLNAYQESGEVLFYEAAEDVWRYIRERQRDTRGGEWFARVRDDGAPYPDDKVDFWKGPYHNGRACLEAIQRLRHLLEHVRSR |
Structure
Sequence
- Sequence statusComplete
- Length412
- Mass (Da)47,387
- Last updated2011-10-19 v1
- Checksum0EEE153737F40DBD
Keywords
- Technical term