F8G0P1 · PNAO_PSEP6
- ProteinPseudooxynicotine dehydrogenase
- Genepnao
- StatusUniProtKB reviewed (Swiss-Prot)
- Amino acids496 (go to sequence)
- Protein existenceEvidence at protein level
- Annotation score5/5
Function
function
Involved in nicotine degradation (PubMed:26634650, PubMed:35835223).
Catalyzes the deamination of pseudooxynicotine to 3-succinoylsemialdehyde-pyridine (PubMed:26634650, PubMed:35835223).
Functions as a dehydrogenase that uses the c-type cytochrome protein CycN as the physiological electron acceptor (PubMed:35835223).
O2 is a poor electron acceptor (PubMed:35835223).
Pnao is oxidized by CycN 230 times faster than O2 at equivalent oxidant concentrations (PubMed:35835223).
Catalyzes the deamination of pseudooxynicotine to 3-succinoylsemialdehyde-pyridine (PubMed:26634650, PubMed:35835223).
Functions as a dehydrogenase that uses the c-type cytochrome protein CycN as the physiological electron acceptor (PubMed:35835223).
O2 is a poor electron acceptor (PubMed:35835223).
Pnao is oxidized by CycN 230 times faster than O2 at equivalent oxidant concentrations (PubMed:35835223).
Catalytic activity
- 2 Fe(III)-[cytochrome c] + H2O + pseudooxynicotine = 4-oxo-4-(pyridin-3-yl)butanal + 2 Fe(II)-[cytochrome c] + 2 H+ + methylamineThis reaction proceeds in the forward direction.
Cofactor
Note: Binds 1 FAD per subunit.
Activity regulation
Strongly inhibited by Na2MoO4 and FeCl3 (PubMed:26634650).
Activity is nearly twice as high in the presence of Na2WO4 (PubMed:26634650).
Activity is nearly twice as high in the presence of Na2WO4 (PubMed:26634650).
Kinetics
KM | SUBSTRATE | pH | TEMPERATURE[C] | NOTES | EVIDENCE | |
---|---|---|---|---|---|---|
0.073 mM | pseudooxynicotine | 30 |
kcat is 0.790 sec-1 (at 30 degrees Celsius).
pH Dependence
Optimum pH is 8.5.
Pathway
Alkaloid degradation; nicotine degradation.
Features
Showing features for binding site.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Binding site | 69 | FAD (UniProtKB | ChEBI) | ||||
Sequence: A | ||||||
Binding site | 88 | FAD (UniProtKB | ChEBI) | ||||
Sequence: E | ||||||
Binding site | 96 | FAD (UniProtKB | ChEBI) | ||||
Sequence: R | ||||||
Binding site | 113 | FAD (UniProtKB | ChEBI) | ||||
Sequence: W | ||||||
Binding site | 285 | FAD (UniProtKB | ChEBI) | ||||
Sequence: V | ||||||
Binding site | 461 | FAD (UniProtKB | ChEBI) | ||||
Sequence: S | ||||||
Binding site | 471 | FAD (UniProtKB | ChEBI) | ||||
Sequence: I |
GO annotations
Aspect | Term | |
---|---|---|
Cellular Component | periplasmic space | |
Molecular Function | oxidoreductase activity | |
Biological Process | alkaloid metabolic process | |
Biological Process | nicotine catabolic process |
Keywords
- Molecular function
- Biological process
- Ligand
Enzyme and pathway databases
Names & Taxonomy
Protein names
- Recommended namePseudooxynicotine dehydrogenase
- EC number
- Alternative names
Gene names
Organism names
- Strain
- Taxonomic lineageBacteria > Pseudomonadota > Gammaproteobacteria > Pseudomonadales > Pseudomonadaceae > Pseudomonas
Accessions
- Primary accessionF8G0P1
Subcellular Location
Phenotypes & Variants
Disruption phenotype
Deletion mutant can degrade nicotine, but it cannot further degrade N-methylmyosime and use nicotine as the sole carbon and nitrogen source.
Features
Showing features for mutagenesis.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Mutagenesis | 180 | Extremely unstable at high temperature. | ||||
Sequence: P → S |
PTM/Processing
Features
Showing features for signal, chain.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Signal | 1-42 | Tat-type signal | ||||
Sequence: MTKDGDEGSKSGVSRRKFLGSAAVGVATAGIASQLLTLSAPA | ||||||
Chain | PRO_0000459073 | 43-496 | Pseudooxynicotine dehydrogenase | |||
Sequence: EAAVKTNVGPSRAGVGYDVIVIGGGFAGVTAAREASRSGLKTLILEGRSRLGGRTFTSKLQNQKVELGGTWVHWTQPNVWTEIMHYGLEVEETVGLANPETVIWVTEDNVKRAPAAEAFEIFGSACNEYYKEARNIYPRPFEPFFERKKLQHVDGLSAADYLEKLPLTREQKDMMDSWLSGNGHNYPETIAYSEIMRWFALSNFNMPTMFDSIARYKIKTGTHSLLEAIMADGNSEVKLSTPVTKVNQDKDKVTVTTEDGVFTASAVIVAVPINTLHDIEYSPKLSAAKVDMGSQRHAGAGVKGYIRVKQNVGNVMTYAPARNKLTPFTSVFTDHVDESGTLLIAFSADPKLIDINDIKAVEKALQPLLPGVEVTASYGYDWNLDPFSKGTWCTYRPNQTTRYLTELQKREGRLFFAGSDMANGWRGFIDGAIENGREVGHQVATYLKRENDNA |
Post-translational modification
Predicted to be exported by the Tat system. The position of the signal peptide cleavage has not been experimentally proven.
Expression
Induction
Expression is up-regulated in the presence of nicotine.
Interaction
Subunit
Homodimer.
Structure
Sequence
- Sequence statusComplete
- Sequence processingThe displayed sequence is further processed into a mature form.
- Length496
- Mass (Da)54,415
- Last updated2011-09-21 v1
- Checksum9EA5F992E35E2433
Keywords
- Technical term
Sequence databases
Nucleotide Sequence | Protein Sequence | Molecule Type | Status | |
---|---|---|---|---|
CP002870 EMBL· GenBank· DDBJ | AEJ14619.1 EMBL· GenBank· DDBJ | Genomic DNA |