F8G0M4 · HSPB_PSEP6
- Protein6-hydroxy-3-succinoylpyridine 3-monooxygenase HspB
- GenehspB
- StatusUniProtKB reviewed (Swiss-Prot)
- Amino acids393 (go to sequence)
- Protein existenceEvidence at protein level
- Annotation score5/5
Function
function
Involved in the nicotine degradation (PubMed:21949128).
Catalyzes the cleavage of 6-hydroxy-3-succinoylpyridine (HSP) by incorporation of oxygen at the 3-position to produce to 2,5-dihydroxypyridine (DHP) and succinic semialdehyde (PubMed:21949128).
Catalyzes the cleavage of 6-hydroxy-3-succinoylpyridine (HSP) by incorporation of oxygen at the 3-position to produce to 2,5-dihydroxypyridine (DHP) and succinic semialdehyde (PubMed:21949128).
Miscellaneous
The catalytic efficiency of HspB is higher than that of HspA.
Catalytic activity
- 4-(6-hydroxypyridin-3-yl)-4-oxobutanoate + 2 H+ + 2 NADH + O2 = 2,5-dihydroxypyridine + H2O + 2 NAD+ + succinate semialdehyde
Cofactor
Note: Binds 1 FAD per subunit.
Activity regulation
Inhibited by Cu2+ and Zn2+.
Kinetics
KM | SUBSTRATE | pH | TEMPERATURE[C] | NOTES | EVIDENCE | |
---|---|---|---|---|---|---|
0.175 mM | HSP | |||||
0.2 mM | NADH |
kcat is 2 sec-1.
pH Dependence
Optimum pH is 8. At pH below 7.0 or above 9.0, there is substantial loss of activity.
Temperature Dependence
Optimum temperature is 25 degrees Celsius. The enzyme activity measured at 5 degrees Celsius is maintained up to 25 degrees Celsius but is lost quickly at higher temperatures.
Pathway
Alkaloid degradation; nicotine degradation.
Features
Showing features for binding site.
GO annotations
Aspect | Term | |
---|---|---|
Molecular Function | FAD binding | |
Molecular Function | monooxygenase activity | |
Biological Process | alkaloid metabolic process | |
Biological Process | nicotine catabolic process |
Keywords
- Molecular function
- Biological process
- Ligand
Enzyme and pathway databases
Names & Taxonomy
Protein names
- Recommended name6-hydroxy-3-succinoylpyridine 3-monooxygenase HspB
- EC number
- Alternative names
Gene names
Organism names
- Strain
- Taxonomic lineageBacteria > Pseudomonadota > Gammaproteobacteria > Pseudomonadales > Pseudomonadaceae > Pseudomonas
Accessions
- Primary accessionF8G0M4
Phenotypes & Variants
Disruption phenotype
Cells lacking this gene are not able to grow in nicotine medium.
PTM/Processing
Features
Showing features for initiator methionine, chain.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Initiator methionine | 1 | Removed | ||||
Sequence: M | ||||||
Chain | PRO_0000422325 | 2-393 | 6-hydroxy-3-succinoylpyridine 3-monooxygenase HspB | |||
Sequence: SMKQRVIIVGGGPVGLLTALGLAKAGTNVVVLEAESQPSDSPRALVYHFPVLPHLKRLGVLDDCVAAGLMRQNFAWRVHSTSEMIFWDLSCLEGDVELPYALHLGQDKLSRILIEHLKALPNVEVRYSSPVVDCEVGPRSVRVVLGGESPGVIVEGDWLIGADGANSFVRREVLNQNFFGITWPQRYVATNTRFDFDKLGFGKTTMQVDDVYGSVICNIDADSLWRVTFMEDPNLPMEGIRGRIDQVFKELLPTNDPYEVVAFSPYRMHQRVTDRMRNGRVILIGDAAHVTNPTGGLGLTGGMFDAFALTSVLNQVIHDGRSEDILDVFEADRRRKFIELVSPRASDNLRNLYHQKPGEGKNDWVNNTRSISKDIDRMRDALRFPETMETFL |
Expression
Induction
Expression is up-regulated in the presence of nicotine.
Interaction
Subunit
Homodimer.
Structure
Sequence
- Sequence statusComplete
- Length393
- Mass (Da)43,971
- Last updated2011-09-21 v1
- ChecksumB650BF5A199C8089
Keywords
- Technical term
Sequence databases
Nucleotide Sequence | Protein Sequence | Molecule Type | Status | |
---|---|---|---|---|
GQ857548 EMBL· GenBank· DDBJ | ADN26547.1 EMBL· GenBank· DDBJ | Genomic DNA | ||
CP002870 EMBL· GenBank· DDBJ | AEJ14602.1 EMBL· GenBank· DDBJ | Genomic DNA |