F7J186 · NVD1_CIOIN
- ProteinCholesterol 7-desaturase nvd 1
- Genenvd-Ci-1
- StatusUniProtKB reviewed (Swiss-Prot)
- Amino acids470 (go to sequence)
- Protein existenceEvidence at protein level
- Annotation score4/5
Function
function
Catalyzes the production of 7-dehydrocholesterol (7-DHC or cholesta-5,7-dien-3beta-ol) by inserting a double bond (desaturating) at the C7-C8 single bond of cholesterol. Essential regulator of steroid biosynthesis as this reaction is the first step in the synthesis of the steroid hormone Delta7-dafachronic acid.
Catalytic activity
- cholesterol + H+ + NADPH + O2 = 7-dehydrocholesterol + 2 H2O + NADP+This reaction proceeds in the forward direction.
Cofactor
Note: Binds 1 [2Fe-2S] cluster per subunit.
Pathway
Steroid hormone biosynthesis; dafachronic acid biosynthesis.
Features
Showing features for binding site.
GO annotations
Aspect | Term | |
---|---|---|
Cellular Component | cytoplasm | |
Cellular Component | membrane | |
Molecular Function | 2 iron, 2 sulfur cluster binding | |
Molecular Function | cholesterol 7-desaturase (NAD(P)H) activity | |
Molecular Function | metal ion binding | |
Molecular Function | oxidoreductase activity | |
Biological Process | cholesterol metabolic process |
Keywords
- Molecular function
- Biological process
- Ligand
Enzyme and pathway databases
Chemistry
Names & Taxonomy
Protein names
- Recommended nameCholesterol 7-desaturase nvd 1
- EC number
- Alternative names
Gene names
Organism names
- Taxonomic lineageEukaryota > Metazoa > Chordata > Tunicata > Ascidiacea > Phlebobranchia > Cionidae > Ciona
Accessions
- Primary accessionF7J186
- Secondary accessions
Proteomes
Subcellular Location
UniProt Annotation
GO Annotation
Membrane ; Single-pass membrane protein
Features
Showing features for transmembrane.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Transmembrane | 67-87 | Helical | ||||
Sequence: LALCIAGFSVLMYFLYVLVFV |
Keywords
- Cellular component
PTM/Processing
Features
Showing features for chain.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Chain | PRO_0000452607 | 1-470 | Cholesterol 7-desaturase nvd 1 | |||
Sequence: MISRIPRLLFENILVPVADVLTKSVSNFAGHKSDQSYNSNATRVSTISDEEIEFYLNLKSAVLLLCLALCIAGFSVLMYFLYVLVFVPYNRVKRLGSIGYQNHEEGHLSKKDIANLVRRRRKVGDDLPPVFPNGWFRLVDSQQLEPGQVKQVTALGEHFAVFRSKSGKASILDAYCPHMGGNLAVGGIVKNDCLECPFHGWRFDGDGKCVAIPYSEKIPTFAKTKSWPCREVNKAIFVWFHCDGKEPEWEIPDISEISTGKFTYKGRVEHHANTHIQDVPENGSDLAHLSHLHVPHAMSGANLSTQYSSWWNFAEHIFKAQCIGPADGEPHISLFYLTHYLHVFKRFKLLSLNLNVYQIGPGIVHLHFDSPFGKGVFVQTLTPVEPLHLVLTHNLHASWSIPVWLGRIFLYLEAIQVDRDVMIWNNKTFEPRPKLLKEDSLIAKYRRWYSQFYTENSPRLTLKSEDGNGW |
Interaction
Protein-protein interaction databases
Structure
Family & Domains
Features
Showing features for domain.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Domain | 136-238 | Rieske | ||||
Sequence: FRLVDSQQLEPGQVKQVTALGEHFAVFRSKSGKASILDAYCPHMGGNLAVGGIVKNDCLECPFHGWRFDGDGKCVAIPYSEKIPTFAKTKSWPCREVNKAIFV |
Sequence similarities
Belongs to the cholesterol 7-desaturase family.
Keywords
- Domain
Phylogenomic databases
Family and domain databases
Sequence
- Sequence statusComplete
- Length470
- Mass (Da)53,548
- Last updated2021-06-02 v2
- Checksum83D5152CFD14105C
Sequence caution
Features
Showing features for sequence conflict.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Sequence conflict | 296 | in Ref. 1; BAK39961 | ||||
Sequence: H → N |
Keywords
- Technical term
Sequence databases
Nucleotide Sequence | Protein Sequence | Molecule Type | Status | |
---|---|---|---|---|
AB607952 EMBL· GenBank· DDBJ | BAK39961.1 EMBL· GenBank· DDBJ | mRNA | ||
EAAA01002278 EMBL· GenBank· DDBJ | - | Genomic DNA | No translation available. |