F7C7C7 · F7C7C7_MACMU

Function

function

Functions as an RNA ligase, in vitro. The ligation reaction entails three nucleotidyl transfer steps. In the first step, the RNA ligase reacts with ATP in the absence of nucleic acid to form a covalent ligase-AMP intermediate and release pyrophosphate. In step 2, the ligase-AMP binds to the nucleic acid and transfers the adenylate to the 5'-PO4 terminus to form an adenylylated intermediate. In step 3, the RNA ligase directs the attack of the 3'-OH on the 5'-phosphoanhydride linkage, resulting in a repaired 3'-5' phosphodiester and release of AMP. Exhibits selectivity for single-stranded RNA substrates and may not have nick-sealing activity on double-stranded DNA-RNA hybrids. May play a role in maintaining RNA integrity under stress conditions, for example in response to reactive oxygen species (ROS).

Catalytic activity

  • ATP + (ribonucleotide)n-3'-hydroxyl + 5'-phospho-(ribonucleotide)m = (ribonucleotide)n+m + AMP + diphosphate.
    EC:6.5.1.3 (UniProtKB | ENZYME | Rhea)

Cofactor

Protein has several cofactor binding sites:
Mg2+ (UniProtKB | Rhea| CHEBI:18420 )

Mn2+ (UniProtKB | Rhea| CHEBI:29035 )

GO annotations

AspectTerm
Molecular FunctionRNA ligase (ATP) activity
Biological Processresponse to reactive oxygen species
Biological ProcessRNA repair

Keywords

  • Molecular function
  • Biological process

Enzyme and pathway databases

Names & Taxonomy

Protein names

  • Recommended name
    RNA ligase 1
  • EC number
  • Alternative names
    • RNA ligase

Gene names

    • Name
      RLIG1

Organism names

  • Taxonomic identifier
  • Strain
    • 17573
  • Taxonomic lineage
    Eukaryota > Metazoa > Chordata > Craniata > Vertebrata > Euteleostomi > Mammalia > Eutheria > Euarchontoglires > Primates > Haplorrhini > Catarrhini > Cercopithecidae > Cercopithecinae > Macaca

Accessions

  • Primary accession
    F7C7C7

Proteomes

Organism-specific databases

Expression

Gene expression databases

Family & Domains

Phylogenomic databases

Family and domain databases

Sequence

  • Sequence status
    Complete
  • Length
    328
  • Mass (Da)
    36,736
  • Last updated
    2019-12-11 v3
  • Checksum
    E24603AEFEA3D0F9
LGRSRGGWARSASGLDPAGDCCRASPQLDWALLESALSTRTACWAHHEALGLRAAENAVCVCDGGERGAFRQKGASDQPYLWARLDRKPNKQAEKRFKNFLHSKENPKEFFWNVEEDFKPAPECWIPAKEIEQINGNPVPDENGHIPGWVPVEKNNKQYCWHSSVVNYEFEIALVLKHHPDDSGLLEISAVPLSDLLEQTLELIGTNINGNPYGLGSKKHPLHLLIPHGAFQVRNLPSLKHNDLLSWFEGCKEGKIEGIVWHCSDGCLIKVHRHHLGLCWPIPDTYMNSKPVIINMNLNKCDSAFDIRFREPGGQRSKGASLCQEIST

Computationally mapped potential isoform sequences

There are 2 potential isoforms mapped to this entry

View all
EntryEntry nameGene nameLength
F7C7B8F7C7B8_MACMURLIG1325
A0A1D5RKK0A0A1D5RKK0_MACMURLIG1329

Keywords

Genome annotation databases

Similar Proteins

Disclaimer

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