F7C7C7 · F7C7C7_MACMU
- ProteinRNA ligase 1
- GeneRLIG1
- StatusUniProtKB unreviewed (TrEMBL)
- Organism
- Amino acids328 (go to sequence)
- Protein existencePredicted
- Annotation score2/5
Function
function
Functions as an RNA ligase, in vitro. The ligation reaction entails three nucleotidyl transfer steps. In the first step, the RNA ligase reacts with ATP in the absence of nucleic acid to form a covalent ligase-AMP intermediate and release pyrophosphate. In step 2, the ligase-AMP binds to the nucleic acid and transfers the adenylate to the 5'-PO4 terminus to form an adenylylated intermediate. In step 3, the RNA ligase directs the attack of the 3'-OH on the 5'-phosphoanhydride linkage, resulting in a repaired 3'-5' phosphodiester and release of AMP. Exhibits selectivity for single-stranded RNA substrates and may not have nick-sealing activity on double-stranded DNA-RNA hybrids. May play a role in maintaining RNA integrity under stress conditions, for example in response to reactive oxygen species (ROS).
Catalytic activity
Cofactor
Protein has several cofactor binding sites:
GO annotations
Aspect | Term | |
---|---|---|
Molecular Function | RNA ligase (ATP) activity | |
Biological Process | response to reactive oxygen species | |
Biological Process | RNA repair |
Keywords
- Molecular function
- Biological process
Enzyme and pathway databases
Names & Taxonomy
Protein names
- Recommended nameRNA ligase 1
- EC number
- Alternative names
Gene names
Organism names
- Organism
- Strain
- Taxonomic lineageEukaryota > Metazoa > Chordata > Craniata > Vertebrata > Euteleostomi > Mammalia > Eutheria > Euarchontoglires > Primates > Haplorrhini > Catarrhini > Cercopithecidae > Cercopithecinae > Macaca
Accessions
- Primary accessionF7C7C7
Proteomes
Organism-specific databases
Expression
Gene expression databases
Structure
Sequence
- Sequence statusComplete
- Length328
- Mass (Da)36,736
- Last updated2019-12-11 v3
- ChecksumE24603AEFEA3D0F9
Computationally mapped potential isoform sequences
There are 2 potential isoforms mapped to this entry
Entry | Entry name | Gene name | Length | ||
---|---|---|---|---|---|
F7C7B8 | F7C7B8_MACMU | RLIG1 | 325 | ||
A0A1D5RKK0 | A0A1D5RKK0_MACMU | RLIG1 | 329 |
Keywords
- Technical term