F5XWJ7 · F5XWJ7_RAMTT
- ProteinDihydroorotate dehydrogenase (quinone)
- GenepyrD
- StatusUniProtKB unreviewed (TrEMBL)
- Amino acids354 (go to sequence)
- Protein existenceInferred from homology
- Annotation score3/5
Function
function
Catalyzes the conversion of dihydroorotate to orotate with quinone as electron acceptor.
Catalytic activity
- (S)-dihydroorotate + a quinone = orotate + a quinol
Cofactor
Note: Binds 1 FMN per subunit.
Pathway
Pyrimidine metabolism; UMP biosynthesis via de novo pathway; orotate from (S)-dihydroorotate (quinone route): step 1/1.
Features
Showing features for binding site, active site.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Binding site | 65-69 | FMN (UniProtKB | ChEBI) | ||||
Sequence: AGLDK | ||||||
Binding site | 69 | substrate | ||||
Sequence: K | ||||||
Binding site | 89 | FMN (UniProtKB | ChEBI) | ||||
Sequence: T | ||||||
Binding site | 114-118 | substrate | ||||
Sequence: NRLGF | ||||||
Binding site | 150 | FMN (UniProtKB | ChEBI) | ||||
Sequence: N | ||||||
Binding site | 183 | FMN (UniProtKB | ChEBI) | ||||
Sequence: N | ||||||
Binding site | 183 | substrate | ||||
Sequence: N | ||||||
Active site | 186 | Nucleophile | ||||
Sequence: S | ||||||
Binding site | 188 | substrate | ||||
Sequence: N | ||||||
Binding site | 228 | FMN (UniProtKB | ChEBI) | ||||
Sequence: K | ||||||
Binding site | 256 | FMN (UniProtKB | ChEBI) | ||||
Sequence: T | ||||||
Binding site | 257-258 | substrate | ||||
Sequence: NT | ||||||
Binding site | 279 | FMN (UniProtKB | ChEBI) | ||||
Sequence: G | ||||||
Binding site | 308 | FMN (UniProtKB | ChEBI) | ||||
Sequence: G | ||||||
Binding site | 329-330 | FMN (UniProtKB | ChEBI) | ||||
Sequence: YT |
GO annotations
Aspect | Term | |
---|---|---|
Cellular Component | cytoplasm | |
Cellular Component | plasma membrane | |
Molecular Function | dihydroorotate dehydrogenase (quinone) activity | |
Biological Process | 'de novo' pyrimidine nucleobase biosynthetic process | |
Biological Process | 'de novo' UMP biosynthetic process |
Keywords
- Molecular function
- Biological process
- Ligand
Enzyme and pathway databases
Names & Taxonomy
Protein names
- Recommended nameDihydroorotate dehydrogenase (quinone)
- EC number
- Alternative names
Gene names
Organism names
- Strain
- Taxonomic lineageBacteria > Pseudomonadota > Betaproteobacteria > Burkholderiales > Comamonadaceae > Ramlibacter
Accessions
- Primary accessionF5XWJ7
Proteomes
Subcellular Location
UniProt Annotation
GO Annotation
Cell membrane ; Peripheral membrane protein
Keywords
- Cellular component
Interaction
Structure
Family & Domains
Features
Showing features for domain.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Domain | 50-340 | Dihydroorotate dehydrogenase catalytic | ||||
Sequence: VELAGLRFPNRVGLAAGLDKNARCIDAFAGMGFGFVEVGTVTPLAQPGNAKPRMFRLPQAQALVNRLGFNNEGLEAFVANVQRSAVRRRSLHDGGIVLGLNIGKNAATPIEDATRDYLTGLEGVYPHADYVTVNISSPNTQNLRALQSDEALDALLAALARRRAELEQRHGRRVPLFVKIAPDLDEAQVRVIAATLQRHGMDGVIATNTTLSRDAVQGLPHAQEAGGLSGAPVLEASNRVIRQLRAALGPRFPIVGVGGVMSGPDAVSKIEAGADLVQVYTGLIYRGPALA |
Sequence similarities
Belongs to the dihydroorotate dehydrogenase family. Type 2 subfamily.
Phylogenomic databases
Family and domain databases
Sequence
- Sequence statusComplete
- Length354
- Mass (Da)38,074
- Last updated2011-07-27 v1
- Checksum0381C85A4F92EF2A
Keywords
- Technical term
Sequence databases
Nucleotide Sequence | Protein Sequence | Molecule Type | Status | |
---|---|---|---|---|
CP000245 EMBL· GenBank· DDBJ | AEG92951.1 EMBL· GenBank· DDBJ | Genomic DNA |