F4KEV7 · FHIT_ARATH

Function

function

Possesses dinucleoside triphosphate hydrolase activity (PubMed:18694747).
Cleaves P1-P3-bis(5'-adenosyl) triphosphate (Ap3A) to yield AMP and ADP (PubMed:18694747).
Exhibits adenylylsulfatase activity, hydrolyzing adenosine 5'-phosphosulfate to yield AMP and sulfate (PubMed:18694747).
Exhibits adenosine 5'-monophosphoramidase activity, hydrolyzing purine nucleotide phosphoramidates with a single phosphate group such as adenosine 5'monophosphoramidate (AMP-NH2) to yield AMP and NH2 (PubMed:18694747).
Exhibits adenylylsulfate-ammonia adenylyltransferase, catalyzing the ammonolysis of adenosine 5'-phosphosulfate resulting in the formation of adenosine 5'-phosphoramidate (PubMed:26181368).

Catalytic activity

Kinetics

KM SUBSTRATE pH TEMPERATURE[C] NOTES EVIDENCE
3 μMadenosine 5'-phosphoramidate6.8

Features

Showing features for binding site, active site.

TypeIDPosition(s)Description
Binding site52substrate
Binding site108substrate
Active site121Tele-AMP-histidine intermediate
Binding site123substrate

GO annotations

AspectTerm
Molecular Functionadenosine 5'-monophosphoramidase activity
Molecular Functionadenylylsulfatase activity
Molecular Functionadenylylsulfate-ammonia adenylyltransferase activity
Molecular Functionbis(5'-adenosyl)-triphosphatase activity
Molecular Functionnucleotide binding

Keywords

Enzyme and pathway databases

Names & Taxonomy

Protein names

  • Recommended name
    Bifunctional bis(5'-adenosyl)-triphosphatase/adenylylsulfatase FHIT
  • EC number
  • Alternative names
    • Adenosine 5'-monophosphoramidase FHIT
      (EC:3.9.1.-
      ) . EC:3.9.1.- (UniProtKB | ENZYME | Rhea)
    • Adenylylsulfatase (EC:3.6.2.1
      ) . EC:3.6.2.1 (UniProtKB | ENZYME | Rhea)
    • Adenylylsulfate-ammonia adenylyltransferase (EC:2.7.7.51
      ) . EC:2.7.7.51 (UniProtKB | ENZYME | Rhea)
    • Fragile histidine triad protein

Gene names

    • Name
      FHIT
    • ORF names
      MCK7.11
    • Ordered locus names
      At5g58240

Organism names

  • Taxonomic identifier
  • Strain
    • cv. Columbia
  • Taxonomic lineage
    Eukaryota > Viridiplantae > Streptophyta > Embryophyta > Tracheophyta > Spermatophyta > Magnoliopsida > eudicotyledons > Gunneridae > Pentapetalae > rosids > malvids > Brassicales > Brassicaceae > Camelineae > Arabidopsis

Accessions

  • Primary accession
    F4KEV7
  • Secondary accessions
    • Q84WL2
    • Q9LVM4

Proteomes

Organism-specific databases

Genome annotation databases

PTM/Processing

Features

Showing features for chain.

TypeIDPosition(s)Description
ChainPRO_00004367451-180Bifunctional bis(5'-adenosyl)-triphosphatase/adenylylsulfatase FHIT

Proteomic databases

PTM databases

Expression

Gene expression databases

Interaction

Protein-protein interaction databases

Structure

Family & Domains

Features

Showing features for domain, motif.

TypeIDPosition(s)Description
Domain27-134HIT
Motif119-123Histidine triad motif

Phylogenomic databases

Family and domain databases

Sequence & Isoform

Align isoforms (2)
  • Sequence status
    Complete

This entry describes 2 isoforms produced by Alternative splicing.

F4KEV7-1

This isoform has been chosen as the canonical sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

  • Length
    180
  • Mass (Da)
    20,400
  • Last updated
    2011-06-28 v1
  • Checksum
    8C88EDB0E9D923FE
MLNLQVTGKTILSSIRCQRKMSSTCSSYAFGPYKIDPREVFYATPLSYAMVNLRPLLPAHVLVCPRRLVPRFTDLTADETSDLWLTAQKVGSKLETFHNASSLTLAIQDGPQAGQTVPHVHIHILPRKGGDFEKNDEIYDALDEKEKELKQKLDLDKDRVDRSIQEMADEASQYRSLFDC

F4KEV7-2

  • Name
    2
  • See also
    sequence in UniParc or sequence clusters in UniRef
  • Differences from canonical

Computationally mapped potential isoform sequences

There is 1 potential isoform mapped to this entry

View all
EntryEntry nameGene nameLength
A0A1P8BCS8A0A1P8BCS8_ARATHFHIT128

Features

Showing features for alternative sequence, sequence conflict.

TypeIDPosition(s)Description
Alternative sequenceVSP_0584131-20in isoform 2
Sequence conflict59in Ref. 3; AAO24535 and 4; BAF00120

Keywords

Sequence databases

Nucleotide SequenceProtein SequenceMolecule TypeStatus
AB019228
EMBL· GenBank· DDBJ
BAA96915.1
EMBL· GenBank· DDBJ
Genomic DNA
CP002688
EMBL· GenBank· DDBJ
AED97022.1
EMBL· GenBank· DDBJ
Genomic DNA
CP002688
EMBL· GenBank· DDBJ
AED97023.1
EMBL· GenBank· DDBJ
Genomic DNA
BT003103
EMBL· GenBank· DDBJ
AAO24535.1
EMBL· GenBank· DDBJ
mRNA
AK228164
EMBL· GenBank· DDBJ
BAF00120.1
EMBL· GenBank· DDBJ
mRNA

Genome annotation databases

Similar Proteins

Disclaimer

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