F4JXF9 · NRPC1_ARATH
- ProteinDNA-directed RNA polymerase III subunit 1
- GeneNRPC1
- StatusUniProtKB reviewed (Swiss-Prot)
- Amino acids1376 (go to sequence)
- Protein existenceEvidence at transcript level
- Annotation score3/5
Function
function
DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates. Largest and catalytic core component of RNA polymerase III which synthesizes small RNAs, such as 5S rRNA and tRNAs. Forms the polymerase active center together with the second largest subunit. A single-stranded DNA template strand of the promoter is positioned within the central active site cleft of Pol III. A bridging helix emanates from NRPC1 and crosses the cleft near the catalytic site and is thought to promote translocation of Pol III by acting as a ratchet that moves the RNA-DNA hybrid through the active site by switching from straight to bent conformations at each step of nucleotide addition.
Catalytic activity
- a ribonucleoside 5'-triphosphate + RNA(n) = diphosphate + RNA(n+1)
Features
Showing features for binding site.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Binding site | 75 | Zn2+ 1 (UniProtKB | ChEBI) | ||||
Sequence: C | ||||||
Binding site | 78 | Zn2+ 1 (UniProtKB | ChEBI) | ||||
Sequence: C | ||||||
Binding site | 85 | Zn2+ 1 (UniProtKB | ChEBI) | ||||
Sequence: C | ||||||
Binding site | 88 | Zn2+ 1 (UniProtKB | ChEBI) | ||||
Sequence: H | ||||||
Binding site | 115 | Zn2+ 2 (UniProtKB | ChEBI) | ||||
Sequence: C | ||||||
Binding site | 118 | Zn2+ 2 (UniProtKB | ChEBI) | ||||
Sequence: C | ||||||
Binding site | 170 | Zn2+ 2 (UniProtKB | ChEBI) | ||||
Sequence: C | ||||||
Binding site | 499 | Mg2+ (UniProtKB | ChEBI); catalytic | ||||
Sequence: D | ||||||
Binding site | 501 | Mg2+ (UniProtKB | ChEBI); catalytic | ||||
Sequence: D | ||||||
Binding site | 503 | Mg2+ (UniProtKB | ChEBI); catalytic | ||||
Sequence: D |
GO annotations
Aspect | Term | |
---|---|---|
Cellular Component | DNA-directed RNA polymerase complex | |
Cellular Component | nucleus | |
Molecular Function | DNA binding | |
Molecular Function | DNA-directed 5'-3' RNA polymerase activity | |
Molecular Function | metal ion binding | |
Biological Process | DNA-templated transcription |
Keywords
- Molecular function
- Biological process
- Ligand
Names & Taxonomy
Protein names
- Recommended nameDNA-directed RNA polymerase III subunit 1
- Alternative names
Gene names
Organism names
- Strain
- Taxonomic lineageEukaryota > Viridiplantae > Streptophyta > Embryophyta > Tracheophyta > Spermatophyta > Magnoliopsida > eudicotyledons > Gunneridae > Pentapetalae > rosids > malvids > Brassicales > Brassicaceae > Camelineae > Arabidopsis
Accessions
- Primary accessionF4JXF9
- Secondary accessions
Proteomes
Organism-specific databases
Genome annotation databases
Subcellular Location
PTM/Processing
Features
Showing features for chain.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Chain | PRO_0000434010 | 1-1376 | DNA-directed RNA polymerase III subunit 1 | |||
Sequence: METKMEIEFTKKPYIEDVGPLKIKSINFSVLSDLEVMKAAEVQVWNIGLYDHSFKPYENGLLDPRMGPPNKKSICTTCEGNFQNCPGHYGYLKLDLPVYNVGYFNFILDILKCICKRCSNMLLDEKLYEDHLRKMRNPRMEPLKKTELAKAVVKKCSTMASQRIITCKKCGYLNGMVKKIAAQFGIGISHDRSKIHGGEIDECKSAISHTKQSTAAINPLTYVLDPNLVLGLFKRMSDKDCELLYIAYRPENLIITCMLVPPLSIRPSVMIGGIQSNENDLTARLKQIILGNASLHKILSQPTSSPKNMQVWDTVQIEVARYINSEVRGCQNQPEEHPLSGILQRLKGKGGRFRANLSGKRVEFTGRTVISPDPNLKITEVGIPILMAQILTFPECVSRHNIEKLRQCVRNGPNKYPGARNVRYPDGSSRTLVGDYRKRIADELAIGCIVDRHLQEGDVVLFNRQPSLHRMSIMCHRARIMPWRTLRFNESVCNPYNADFDGDEMNMHVPQTEEARTEAITLMGVQNNLCTPKNGEILVASTQDFLTSSFLITRKDTFYDRAAFSLICSYMGDGMDSIDLPTPTILKPIELWTGKQIFSVLLRPNASIRVYVTLNVKEKNFKKGEHGFDETMCINDGWVYFRNSELISGQLGKATLGNGNKDGLYSILLRDYNSHAAAVCMNRLAKLSARWIGIHGFSIGIDDVQPGEELSKERKDSIQFGYDQCHRKIEEFNRGNLQLKAGLDGAKSLEAEITGILNTIREATGKACMSGLHWRNSPLIMSQCGSKGSPINISQMVACVGQQTVNGHRAPDGFIDRSLPHFPRMSKSPAAKGFVANSFYSGLTATEFFFHTMGGREGLVDTAVKTASTGYMSRRLMKALEDLLVHYDNTVRNASGCILQFTYGDDGMDPALMEGKDGAPLNFNRLFLKVQATCPPRSHHTYLSSEELSQKFEEELVRHDKSRVCTDAFVKSLREFVSLLGVKSASPPQVLYKASGVTDKQLEVFVKICVFRYREKKIEAGTAIGTIGAQSIGEPGTQMTLKTFHFAGVASMNITQGVPRINEIINASKNISTPVISAELENPLELTSARWVKGRIEKTTLGQVAESIEVLMTSTSASVRIILDNKIIEEACLSITPWSVKNSILKTPRIKLNDNDIRVLDTGLDITPVVDKSRAHFNLHNLKNVLPNIIVNGIKTVERVVVAEDMDKSKQIDGKTKWKLFVEGTNLLAVMGTPGINGRTTTSNNVVEVSKTLGIEAARTTIIDEIGTVMGNHGMSIDIRHMMLLADVMTYRGEVLGIQRTGIQKMDKSVLMQASFERTGDHLFSAAASGKVDNIEGVTECVIMGIPMKLGTGILKVLQRTDDLPKLKYGPDPIIS |
Proteomic databases
PTM databases
Expression
Gene expression databases
Interaction
Subunit
Component of the RNA polymerase III (Pol III) complex consisting of 17 subunits.
Protein-protein interaction databases
Structure
Family & Domains
Features
Showing features for region.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Region | 845-857 | Bridging helix | ||||
Sequence: ATEFFFHTMGGRE |
Sequence similarities
Belongs to the RNA polymerase beta' chain family.
Phylogenomic databases
Family and domain databases
Sequence
- Sequence statusComplete
This entry describes 1 isoforms produced by Alternative splicing. A number of isoforms are produced. According to EST sequences.
F4JXF9-1
This isoform has been chosen as the canonical sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.
- Name1
- Length1,376
- Mass (Da)153,006
- Last updated2011-06-28 v1
- Checksum12B86B2A04751C4E
Computationally mapped potential isoform sequences
There is 1 potential isoform mapped to this entry
Entry | Entry name | Gene name | Length | ||
---|---|---|---|---|---|
F4JXF8 | F4JXF8_ARATH | NRPC1 | 1391 |
Sequence caution
Keywords
- Coding sequence diversity
- Technical term
Sequence databases
Nucleotide Sequence | Protein Sequence | Molecule Type | Status | |
---|---|---|---|---|
AB019231 EMBL· GenBank· DDBJ | BAA96933.1 EMBL· GenBank· DDBJ | Genomic DNA | Sequence problems. | |
CP002688 EMBL· GenBank· DDBJ | AED97269.1 EMBL· GenBank· DDBJ | Genomic DNA | ||
AK229965 EMBL· GenBank· DDBJ | - | mRNA | No translation available. | |
AK230117 EMBL· GenBank· DDBJ | - | mRNA | No translation available. |