F4JNX3 · CMKMT_ARATH
- ProteinCalmodulin-lysine N-methyltransferase
- GeneCaMKMT
- StatusUniProtKB reviewed (Swiss-Prot)
- Amino acids304 (go to sequence)
- Protein existenceEvidence at transcript level
- Annotation score5/5
Function
function
Catalyzes the trimethylation of calmodulin (PubMed:24285794).
Regulates roots development probably by modulating auxin signaling responses. May be involved in gravitropism. Involved in abscisic acid (ABA)-mediated and abiotic stress responses, including salt (NaCl), cold, drought and heat stresses (PubMed:24285794).
Regulates roots development probably by modulating auxin signaling responses. May be involved in gravitropism. Involved in abscisic acid (ABA)-mediated and abiotic stress responses, including salt (NaCl), cold, drought and heat stresses (PubMed:24285794).
Catalytic activity
- [calmodulin]-L-lysine + S-adenosyl-L-methionine = [calmodulin]-N6-methyl-L-lysine + H+ + S-adenosyl-L-homocysteine
GO annotations
all annotations | all molecular function | nucleotide binding | molecular_function | nucleic acid binding | dna binding | chromatin binding | dna-binding transcription factor activity | rna binding | cytoskeletal motor activity | catalytic activity | nuclease activity | signaling receptor binding | structural molecule activity | transporter activity | binding | protein binding | translation factor activity, rna binding | lipid binding | kinase activity | transferase activity | hydrolase activity | oxygen binding | enzyme regulator activity | carbohydrate binding | signaling receptor activity | translation regulator activity | transcription regulator activity | other molecular function | all biological process | carbohydrate metabolic process | generation of precursor metabolites and energy | nucleobase-containing compound metabolic process | dna metabolic process | translation | lipid metabolic process | transport | response to stress | cell cycle | cell communication | signal transduction | cell-cell signaling | multicellular organism development | circadian rhythm | biological_process | metabolic process | catabolic process | biosynthetic process | response to light stimulus | response to external stimulus | tropism | response to biotic stimulus | response to abiotic stimulus | response to endogenous stimulus | embryo development | post-embryonic development | fruit ripening | abscission | pollination | flower development | cellular process | programmed cell death | photosynthesis | cellular component organization | cell growth | protein metabolic process | cellular homeostasis | secondary metabolic process | reproductive process | cell differentiation | protein modification process | growth | epigenetic regulation of gene expression | response to chemical | anatomical structure development | regulation of molecular function | other biological process | all cellular component | cellular_component | extracellular region | cell wall | intracellular anatomical structure | nucleus | nuclear envelope | nucleoplasm | nucleolus | cytoplasm | mitochondrion | lysosome | endosome | vacuole | peroxisome | endoplasmic reticulum | golgi apparatus | cytosol | ribosome | cytoskeleton | plasma membrane | chloroplast | plastid | thylakoid | membrane | external encapsulating structure | other cellular component | |||
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Aspect | Term | |
---|---|---|
Cellular Component | cytoplasm | |
Cellular Component | nucleus | |
Molecular Function | calmodulin-lysine N-methyltransferase activity | |
Biological Process | abscisic acid-activated signaling pathway | |
Biological Process | auxin-activated signaling pathway | |
Biological Process | gravitropism | |
Biological Process | methylation | |
Biological Process | regulation of abscisic acid-activated signaling pathway | |
Biological Process | regulation of auxin mediated signaling pathway | |
Biological Process | regulation of response to salt stress | |
Biological Process | regulation of response to water deprivation | |
Biological Process | regulation of root development | |
Biological Process | response to abscisic acid | |
Biological Process | response to auxin | |
Biological Process | response to cold | |
Biological Process | response to heat | |
Biological Process | response to oomycetes | |
Biological Process | response to osmotic stress | |
Biological Process | response to salt | |
Biological Process | response to salt stress | |
Biological Process | root hair cell development |
Keywords
- Molecular function
- Biological process
Enzyme and pathway databases
Names & Taxonomy
Protein names
- Recommended nameCalmodulin-lysine N-methyltransferase
- EC number
- Short namesCLNMT ; CaM KMT
Gene names
Organism names
- Strain
- Taxonomic lineageEukaryota > Viridiplantae > Streptophyta > Embryophyta > Tracheophyta > Spermatophyta > Magnoliopsida > eudicotyledons > Gunneridae > Pentapetalae > rosids > malvids > Brassicales > Brassicaceae > Camelineae > Arabidopsis
Accessions
- Primary accessionF4JNX3
- Secondary accessions
Proteomes
Organism-specific databases
Genome annotation databases
Phenotypes & Variants
Disruption phenotype
Suppressed calmodulin (CaM) methylation, especially in roots. Longer roots with ectopic root hair cells in atrichoblast cell files and the presence of nonhair cells in trichoblast cell files. Increased number of epidermal cells in the root elongation zone. Reduced root growth inhibition mediated by auxins indole-3-acetic acid (IAA) and 2,4-dichlorophenoxyacetic acid (2,4-D). Reduced sensitivity to abscisic acid (ABA)-mediated stress leading to green cotyledons and normal germination in the presence of ABA. Increased tolerance to abiotic stress such as salt (NaCl), cold, drought and heat stresses.
Variants
We now provide the "Disease & Variants" viewer in its own tab.
The viewer provides 29 variants from UniProt as well as other sources including ClinVar and dbSNP.
PTM/Processing
Features
Showing features for chain.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Chain | PRO_0000445938 | 1-304 | Calmodulin-lysine N-methyltransferase | |||
Sequence: MDPTSSSSSALRWKILRQALLRRSDSQSQTETKRISRKATQGFNLIPCQVVDSSPQSDKSREASVCYTLPITGSPKLYLTQRVDNCSDLNDFEISNRYNIDNTGLVCQWPSEEVLAYFCKSQPERFRGKRVIELGSGYGLAGLVIAAATEASEVVISDGNPQVVNYIKRNIETNSMAFGGTSVKAMELHWNQHQLSELTNTFDIIVASDCTFFKEFHKDLARTIKMLLKAKKASEALFFSPKRGDSLEKFMKEIKDIGLHYILTENYDAQVWKRHETLVKGDEAWPNYDKNHCYPLLIQITNQI |
Proteomic databases
Expression
Tissue specificity
Expressed in discreet spatial and tissue-specific patterns including root tips, leaves-tips, floral buds, stamens, hydathodes, stigma, anther, siliques, apical meristems and germinating seeds (PubMed:24260266, PubMed:24285794).
Also observed at high levels in the root stele region (PubMed:24260266).
Also observed at high levels in the root stele region (PubMed:24260266).
Induction
Induced by salt (NaCl) stress (PubMed:24260266, PubMed:24285794).
Accumulates in response to osmotic stress (e.g. mannitol) and upon biotic stress, e.g. inoculation with the oomycete P.tabacina (PubMed:24260266).
Triggered by auxins indole-3-acetic acid (IAA) and 2,4-dichlorophenoxyacetic acid (2,4-D). Repressed by kinetin or abscisic acid (ABA) treatments (PubMed:24285794).
Accumulates in response to osmotic stress (e.g. mannitol) and upon biotic stress, e.g. inoculation with the oomycete P.tabacina (PubMed:24260266).
Triggered by auxins indole-3-acetic acid (IAA) and 2,4-dichlorophenoxyacetic acid (2,4-D). Repressed by kinetin or abscisic acid (ABA) treatments (PubMed:24285794).
Developmental stage
After seed stratification, first observed in the micropylar end, later present in the endosperm region and in the testa, and finally accumulates in the endosperm and emerging radicle. In seedlings, first detected in the root and cotyledon tips, expression level reaches a maximal at the cotyledonary leaf stage and is later confined in the shoot apical meristem. In roots, expressed at the primary root tip, at the basal side of root curvature, and at the lateral root tip (PubMed:24285794).
Also observed in very young primordia, floral buds, and stamens (PubMed:24260266, PubMed:24285794).
In stamens, mostly present in the anther containing immature pollen and, at low levels, in the filament. In siliques, highly expressed in the abscission zone and, to a lower extent, in developing seeds (PubMed:24285794).
Also observed in very young primordia, floral buds, and stamens (PubMed:24260266, PubMed:24285794).
In stamens, mostly present in the anther containing immature pollen and, at low levels, in the filament. In siliques, highly expressed in the abscission zone and, to a lower extent, in developing seeds (PubMed:24285794).
Gene expression databases
Interaction
Structure
Family & Domains
Sequence similarities
Belongs to the class I-like SAM-binding methyltransferase superfamily. CLNMT methyltransferase family.
Phylogenomic databases
Family and domain databases
Sequence & Isoform
- Sequence statusComplete
This entry describes 2 isoforms produced by Alternative splicing.
F4JNX3-1
This isoform has been chosen as the canonical sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.
- Name1
- Length304
- Mass (Da)34,491
- Last updated2011-06-28 v1
- ChecksumB5A77C6FD0AA0484
F4JNX3-2
- Name2
- Differences from canonical
- 1-175: Missing
Features
Showing features for alternative sequence.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Alternative sequence | VSP_059985 | 1-175 | in isoform 2 | |||
Sequence: Missing |
Keywords
- Coding sequence diversity
- Technical term
Sequence databases
Nucleotide Sequence | Protein Sequence | Molecule Type | Status | |
---|---|---|---|---|
AL022373 EMBL· GenBank· DDBJ | CAA18493.1 EMBL· GenBank· DDBJ | Genomic DNA | ||
AL161588 EMBL· GenBank· DDBJ | CAB81508.1 EMBL· GenBank· DDBJ | Genomic DNA | ||
CP002687 EMBL· GenBank· DDBJ | AEE86599.1 EMBL· GenBank· DDBJ | Genomic DNA | ||
BT029304 EMBL· GenBank· DDBJ | ABK32118.1 EMBL· GenBank· DDBJ | mRNA |