F4J4C8 · EVN_ARATH
- ProteinDolichol kinase EVAN
- GeneEVN
- StatusUniProtKB reviewed (Swiss-Prot)
- Amino acids569 (go to sequence)
- Protein existenceEvidence at transcript level
- Annotation score4/5
Function
function
Essential for pollen development. Involved in protein N-glycosylation in the endoplasmic reticulum (ER), especially in the female gametophyte. Mediates pollen tube (PT) reception in synergids through protein glycosylation.
Catalytic activity
- a di-trans,poly-cis-dolichol + CTP = a di-trans,poly-cis-dolichyl phosphate + CDP + H+
a di-trans,poly-cis-dolichol RHEA-COMP:19495
+ CHEBI:37563 = a di-trans,poly-cis-dolichyl phosphate RHEA-COMP:19498
+ CHEBI:58069 + CHEBI:15378
GO annotations
all annotations | all molecular function | nucleotide binding | molecular_function | nucleic acid binding | dna binding | chromatin binding | dna-binding transcription factor activity | rna binding | cytoskeletal motor activity | catalytic activity | nuclease activity | signaling receptor binding | structural molecule activity | transporter activity | binding | protein binding | translation factor activity, rna binding | lipid binding | kinase activity | transferase activity | hydrolase activity | oxygen binding | enzyme regulator activity | carbohydrate binding | signaling receptor activity | translation regulator activity | transcription regulator activity | other molecular function | all biological process | carbohydrate metabolic process | generation of precursor metabolites and energy | nucleobase-containing compound metabolic process | dna metabolic process | translation | lipid metabolic process | transport | response to stress | cell cycle | cell communication | signal transduction | cell-cell signaling | multicellular organism development | circadian rhythm | biological_process | metabolic process | catabolic process | biosynthetic process | response to light stimulus | response to external stimulus | tropism | response to biotic stimulus | response to abiotic stimulus | response to endogenous stimulus | embryo development | post-embryonic development | fruit ripening | abscission | pollination | flower development | cellular process | programmed cell death | photosynthesis | cellular component organization | cell growth | protein metabolic process | cellular homeostasis | secondary metabolic process | reproductive process | cell differentiation | protein modification process | growth | epigenetic regulation of gene expression | response to chemical | anatomical structure development | regulation of molecular function | other biological process | all cellular component | cellular_component | extracellular region | cell wall | intracellular anatomical structure | nucleus | nuclear envelope | nucleoplasm | nucleolus | cytoplasm | mitochondrion | lysosome | endosome | vacuole | peroxisome | endoplasmic reticulum | golgi apparatus | cytosol | ribosome | cytoskeleton | plasma membrane | chloroplast | plastid | thylakoid | membrane | external encapsulating structure | other cellular component | |||
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Aspect | Term | |
---|---|---|
Cellular Component | endoplasmic reticulum | |
Cellular Component | endoplasmic reticulum membrane | |
Molecular Function | dolichol kinase activity | |
Biological Process | pollen development | |
Biological Process | pollen tube reception | |
Biological Process | protein glycosylation |
Keywords
- Molecular function
Enzyme and pathway databases
Names & Taxonomy
Protein names
- Recommended nameDolichol kinase EVAN
- EC number
Gene names
Organism names
- Strain
- Taxonomic lineageEukaryota > Viridiplantae > Streptophyta > Embryophyta > Tracheophyta > Spermatophyta > Magnoliopsida > eudicotyledons > Gunneridae > Pentapetalae > rosids > malvids > Brassicales > Brassicaceae > Camelineae > Arabidopsis
Accessions
- Primary accessionF4J4C8
- Secondary accessions
Proteomes
Organism-specific databases
Genome annotation databases
Subcellular Location
UniProt Annotation
GO Annotation
Endoplasmic reticulum membrane ; Multi-pass membrane protein
Features
Showing features for topological domain, transmembrane.
Type | ID | Position(s) | Description | ||
---|---|---|---|---|---|
Topological domain | 1-22 | Cytoplasmic | |||
Transmembrane | 23-43 | Helical | |||
Topological domain | 44-67 | Lumenal | |||
Transmembrane | 68-88 | Helical | |||
Topological domain | 89-108 | Cytoplasmic | |||
Transmembrane | 109-129 | Helical | |||
Topological domain | 130-147 | Lumenal | |||
Transmembrane | 148-168 | Helical | |||
Topological domain | 169-178 | Cytoplasmic | |||
Transmembrane | 179-199 | Helical | |||
Topological domain | 200-207 | Lumenal | |||
Transmembrane | 208-228 | Helical | |||
Topological domain | 229-252 | Cytoplasmic | |||
Transmembrane | 253-273 | Helical | |||
Topological domain | 274-296 | Lumenal | |||
Transmembrane | 297-317 | Helical | |||
Topological domain | 318-340 | Cytoplasmic | |||
Transmembrane | 341-361 | Helical | |||
Topological domain | 362-369 | Lumenal | |||
Transmembrane | 370-390 | Helical | |||
Topological domain | 391-393 | Cytoplasmic | |||
Transmembrane | 394-414 | Helical | |||
Topological domain | 415-440 | Lumenal | |||
Transmembrane | 441-461 | Helical | |||
Topological domain | 462-464 | Cytoplasmic | |||
Transmembrane | 465-485 | Helical | |||
Topological domain | 486-508 | Lumenal | |||
Transmembrane | 509-529 | Helical | |||
Topological domain | 530-548 | Cytoplasmic | |||
Transmembrane | 549-569 | Helical | |||
Keywords
- Cellular component
Phenotypes & Variants
Disruption phenotype
Pollen tube (PT) overgrowth inside the female gametophyte (FG) without PT rupture. Degenerated pollen grains before maturation, during the early tricellular stage.
Variants
![](/variants.8e7f84.jpg)
We now provide the "Disease & Variants" viewer in its own tab.
The viewer provides 60 variants from UniProt as well as other sources including ClinVar and dbSNP.
Expression
Gene expression databases
Interaction
Protein-protein interaction databases
Structure
Family & Domains
Features
Showing features for region.
Type | ID | Position(s) | Description | ||
---|---|---|---|---|---|
Region | 487-503 | CTP-binding | |||
Sequence similarities
Belongs to the polyprenol kinase family.
Keywords
- Domain
Phylogenomic databases
Family and domain databases
Sequence
- Sequence statusComplete
- Length569
- Mass (Da)63,136
- Last updated2011-06-28 v1
- MD5 Checksum3821F6C31993DE5F1D0B04BC9E774F67
Computationally mapped potential isoform sequences
There are 2 potential isoforms mapped to this entry
Entry | Entry name | Gene name | Length | ||
---|---|---|---|---|---|
A0A1I9LMJ1 | A0A1I9LMJ1_ARATH | DOK1 | 521 | ||
A0A1I9LMJ2 | A0A1I9LMJ2_ARATH | DOK1 | 552 |
Sequence caution
Features
Showing features for sequence conflict.
Type | ID | Position(s) | Description | ||
---|---|---|---|---|---|
Sequence conflict | 411 | in Ref. 3; AAL67067 | |||
Sequence conflict | 419 | in Ref. 3; AAL67067 | |||
Keywords
- Technical term
Sequence databases
Nucleotide Sequence | Protein Sequence | Molecule Type | Status | |
---|---|---|---|---|
AL353992 EMBL· GenBank· DDBJ | CAB89319.1 EMBL· GenBank· DDBJ | Genomic DNA | Sequence problems. | |
CP002686 EMBL· GenBank· DDBJ | AEE77982.1 EMBL· GenBank· DDBJ | Genomic DNA | ||
AY074371 EMBL· GenBank· DDBJ | AAL67067.1 EMBL· GenBank· DDBJ | mRNA |