F4I907 · GLYR2_ARATH
- ProteinGlyoxylate/succinic semialdehyde reductase 2, chloroplastic
- GeneGLYR2
- StatusUniProtKB reviewed (Swiss-Prot)
- Amino acids358 (go to sequence)
- Protein existenceEvidence at protein level
- Annotation score5/5
Function
function
Catalyzes the NADPH-dependent reduction of glyoxylate to glycolate as well as succinic semialdehyde (SSA) to gamma-hydroxybutyrate in vitro. May function in redox homeostasis and play a role in oxidative stress tolerance by detoxifying glyoxylate and SSA generated in glycolate metabolism and GABA metabolism, respectively.
Miscellaneous
Although GLYR2 acts as an aldo/keto reductase, it has no significant homology with either mammalian and bacterial NADPH-dependent SSA reductases.
Catalytic activity
- glycolate + NADP+ = glyoxylate + H+ + NADPH
Activity regulation
The ratio of NADPH/NADP+ may regulate enzymatic activity.
Kinetics
KM | SUBSTRATE | pH | TEMPERATURE[C] | NOTES | EVIDENCE | |
---|---|---|---|---|---|---|
34 μM | glyoxylate (check for NADPH) | |||||
8.96 mM | succinate semialdehyde | |||||
34 μM | NADPH | in the presence of glyoxylate as cosubstrate | ||||
12 μM | NADPH | in the presence of succinate semialdehyde as cosubstrate |
Vmax | pH | TEMPERATURE[C] | NOTES | EVIDENCE | |
---|---|---|---|---|---|
40.6 μmol/min/mg | toward glyoxylate | ||||
30.7 μmol/min/mg | toward succinate semialdehyde |
pH Dependence
Optimum pH is 6.8-7.8.
Features
Showing features for binding site, active site.
GO annotations
Aspect | Term | |
---|---|---|
Cellular Component | chloroplast | |
Cellular Component | chloroplast stroma | |
Molecular Function | glyoxylate reductase (NADPH) activity | |
Molecular Function | NAD binding | |
Molecular Function | NADP binding |
Keywords
- Molecular function
- Biological process
- Ligand
Enzyme and pathway databases
Names & Taxonomy
Protein names
- Recommended nameGlyoxylate/succinic semialdehyde reductase 2, chloroplastic
- EC number
- Short namesAtGLYR2; AtGR2; SSA reductase 2
Gene names
Organism names
- Strain
- Taxonomic lineageEukaryota > Viridiplantae > Streptophyta > Embryophyta > Tracheophyta > Spermatophyta > Magnoliopsida > eudicotyledons > Gunneridae > Pentapetalae > rosids > malvids > Brassicales > Brassicaceae > Camelineae > Arabidopsis
Accessions
- Primary accessionF4I907
- Secondary accessions
Proteomes
Organism-specific databases
Genome annotation databases
Subcellular Location
PTM/Processing
Features
Showing features for transit peptide, chain.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Transit peptide | 1-44 | Chloroplast | ||||
Sequence: MPLVSLSFASSSSKAMALCSICPRIPLRFRPKPISPFLSKPQIC | ||||||
Chain | PRO_0000421033 | 45-358 | Glyoxylate/succinic semialdehyde reductase 2, chloroplastic | |||
Sequence: LAYRVYSSLQSTTPSTRDELGTVSIGFLGMGIMGSPMAQNLIKAGCDVTVWNRTKSKCDPLVGLGAKYKSSPEEVTATCDLTFAMLADPESAIDVACGKNGAIFGISSGKGYVDVSTVDVASSILISKQIKDTGALFLEAPVSGSKKPAEDGQLIFLTAGDKPLYEKAAPFLDIMGKSKFYLGEVGNGAAMKLVVNMIMGSMMASFAEGILLSQKVGLDPNVLVEVVSQGAINAPMYSLKGPSMIKSVYPTAFPLKHQQKDMRLALGLAESVSQSTPIAAAANELYKVAKSYGLSDEDFSAVIEALKAAKSREA |
Proteomic databases
PTM databases
Expression
Induction
By cold and heat stresses. Down-regulated by ozone.
Gene expression databases
Interaction
Binary interactions
Type | Entry 1 | Entry 2 | Number of experiments | Intact | |
---|---|---|---|---|---|
BINARY | F4I907 | SCL3 Q9LPR8 | 3 | EBI-4453651, EBI-4429250 |
Protein-protein interaction databases
Structure
Family & Domains
Sequence similarities
Belongs to the HIBADH-related family. NP60 subfamily.
Keywords
- Domain
Phylogenomic databases
Family and domain databases
Sequence
- Sequence statusComplete
- Sequence processingThe displayed sequence is further processed into a mature form.
- Length358
- Mass (Da)37,781
- Last updated2011-06-28 v1
- ChecksumC46D7A0338B5BF3B
Computationally mapped potential isoform sequences
There is 1 potential isoform mapped to this entry
Entry | Entry name | Gene name | Length | ||
---|---|---|---|---|---|
A0A1P8AS42 | A0A1P8AS42_ARATH | GLYR2 | 299 |
Keywords
- Technical term
Sequence databases
Nucleotide Sequence | Protein Sequence | Molecule Type | Status | |
---|---|---|---|---|
AC034257 EMBL· GenBank· DDBJ | - | Genomic DNA | No translation available. | |
CP002684 EMBL· GenBank· DDBJ | AEE29618.1 EMBL· GenBank· DDBJ | Genomic DNA | ||
AY085690 EMBL· GenBank· DDBJ | AAM62909.1 EMBL· GenBank· DDBJ | mRNA | ||
AY093135 EMBL· GenBank· DDBJ | AAM13134.1 EMBL· GenBank· DDBJ | mRNA | ||
BT008734 EMBL· GenBank· DDBJ | AAP42747.1 EMBL· GenBank· DDBJ | mRNA |