F4B9S5 · F4B9S5_ACIHW

Function

function

Small subunit of the glutamine-dependent carbamoyl phosphate synthetase (CPSase). CPSase catalyzes the formation of carbamoyl phosphate from the ammonia moiety of glutamine, carbonate, and phosphate donated by ATP, constituting the first step of 2 biosynthetic pathways, one leading to arginine and/or urea and the other to pyrimidine nucleotides. The small subunit (glutamine amidotransferase) binds and cleaves glutamine to supply the large subunit with the substrate ammonia.

Catalytic activity

Pathway

Amino-acid biosynthesis; L-arginine biosynthesis; carbamoyl phosphate from bicarbonate: step 1/1.
Pyrimidine metabolism; UMP biosynthesis via de novo pathway; (S)-dihydroorotate from bicarbonate: step 1/3.

Features

Showing features for binding site, active site.

TypeIDPosition(s)Description
Binding site51L-glutamine (UniProtKB | ChEBI)
Binding site232L-glutamine (UniProtKB | ChEBI)
Binding site234L-glutamine (UniProtKB | ChEBI)
Active site260Nucleophile
Binding site261L-glutamine (UniProtKB | ChEBI)
Binding site264L-glutamine (UniProtKB | ChEBI)
Binding site302L-glutamine (UniProtKB | ChEBI)
Binding site304L-glutamine (UniProtKB | ChEBI)
Binding site305L-glutamine (UniProtKB | ChEBI)
Active site344
Active site346

GO annotations

AspectTerm
Molecular FunctionATP binding
Molecular Functioncarbamoyl-phosphate synthase (glutamine-hydrolyzing) activity
Molecular Functionglutaminase activity
Biological Process'de novo' pyrimidine nucleobase biosynthetic process
Biological Process'de novo' UMP biosynthetic process
Biological Processarginine biosynthetic process
Biological Processglutamine metabolic process

Keywords

Enzyme and pathway databases

Protein family/group databases

Names & Taxonomy

Protein names

  • Recommended name
    Carbamoyl phosphate synthase small chain
  • EC number
  • Alternative names
    • Carbamoyl phosphate synthetase glutamine chain

Gene names

    • Name
      carA
    • Ordered locus names
      Ahos_1107

Organism names

Accessions

  • Primary accession
    F4B9S5

Proteomes

Interaction

Subunit

Composed of two chains; the small (or glutamine) chain promotes the hydrolysis of glutamine to ammonia, which is used by the large (or ammonia) chain to synthesize carbamoyl phosphate. Tetramer of heterodimers (alpha,beta)4.

Protein-protein interaction databases

Family & Domains

Features

Showing features for region, domain.

TypeIDPosition(s)Description
Region1-182CPSase
Domain7-144Carbamoyl-phosphate synthase small subunit N-terminal

Sequence similarities

Belongs to the CarA family.
Belongs to the CarB family.

Keywords

Phylogenomic databases

Family and domain databases

Sequence

  • Sequence status
    Complete
  • Length
    370
  • Mass (Da)
    41,360
  • Last updated
    2011-06-28 v1
  • Checksum
    A9092DA9BE227015
MICKRGYKGYIYLEDGTLIEGCGFGAKGIKAGEVVFTTSMNGYPESLTDPSYRGQILVITHPLVGNYGIPKPIVENGILRNFESERIQVEGLVVAEETDPYKWNSQMSLHDWLKSEGVPGVSDVDTRMLVKKVRNKGVMMGVISSGIELDDPKKYLEKKYDEIDFTQFTSPKSPIIHQGNGKGIIVLVDCGIKHGILYQLNSRGYTVVRVPCNFTANQIMDYSPQGVVFGNGPGNPKLLQGLIKTYKDIVEYKVPIMGICLGHQITSLALGGNVRKMKYGHRAINKPVIDVTDNKCYITTHNHGYGVFKEDVPKDMKVWFYNPDDGVVEGMIHNKLPIITTQFHPEARPGPWDVTWVFDKFKKVIESGRP

Sequence databases

Nucleotide SequenceProtein SequenceMolecule TypeStatus
CP002535
EMBL· GenBank· DDBJ
AEE93991.1
EMBL· GenBank· DDBJ
Genomic DNA

Genome annotation databases

Similar Proteins

Disclaimer

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