F4B9S5 · F4B9S5_ACIHW
- ProteinCarbamoyl phosphate synthase small chain
- GenecarA
- StatusUniProtKB unreviewed (TrEMBL)
- Organism
- Amino acids370 (go to sequence)
- Protein existenceInferred from homology
- Annotation score4/5
Function
function
Small subunit of the glutamine-dependent carbamoyl phosphate synthetase (CPSase). CPSase catalyzes the formation of carbamoyl phosphate from the ammonia moiety of glutamine, carbonate, and phosphate donated by ATP, constituting the first step of 2 biosynthetic pathways, one leading to arginine and/or urea and the other to pyrimidine nucleotides. The small subunit (glutamine amidotransferase) binds and cleaves glutamine to supply the large subunit with the substrate ammonia.
Catalytic activity
- 2 ATP + H2O + hydrogencarbonate + L-glutamine = 2 ADP + carbamoyl phosphate + 2 H+ + L-glutamate + phosphate
- H2O + L-glutamine = L-glutamate + NH4+
Pathway
Amino-acid biosynthesis; L-arginine biosynthesis; carbamoyl phosphate from bicarbonate: step 1/1.
Pyrimidine metabolism; UMP biosynthesis via de novo pathway; (S)-dihydroorotate from bicarbonate: step 1/3.
Features
Showing features for binding site, active site.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Binding site | 51 | L-glutamine (UniProtKB | ChEBI) | ||||
Sequence: S | ||||||
Binding site | 232 | L-glutamine (UniProtKB | ChEBI) | ||||
Sequence: G | ||||||
Binding site | 234 | L-glutamine (UniProtKB | ChEBI) | ||||
Sequence: G | ||||||
Active site | 260 | Nucleophile | ||||
Sequence: C | ||||||
Binding site | 261 | L-glutamine (UniProtKB | ChEBI) | ||||
Sequence: L | ||||||
Binding site | 264 | L-glutamine (UniProtKB | ChEBI) | ||||
Sequence: Q | ||||||
Binding site | 302 | L-glutamine (UniProtKB | ChEBI) | ||||
Sequence: N | ||||||
Binding site | 304 | L-glutamine (UniProtKB | ChEBI) | ||||
Sequence: G | ||||||
Binding site | 305 | L-glutamine (UniProtKB | ChEBI) | ||||
Sequence: Y | ||||||
Active site | 344 | |||||
Sequence: H | ||||||
Active site | 346 | |||||
Sequence: E |
GO annotations
Aspect | Term | |
---|---|---|
Molecular Function | ATP binding | |
Molecular Function | carbamoyl-phosphate synthase (glutamine-hydrolyzing) activity | |
Molecular Function | glutaminase activity | |
Biological Process | 'de novo' pyrimidine nucleobase biosynthetic process | |
Biological Process | 'de novo' UMP biosynthetic process | |
Biological Process | arginine biosynthetic process | |
Biological Process | glutamine metabolic process |
Keywords
- Molecular function
- Biological process
- Ligand
Enzyme and pathway databases
Protein family/group databases
Names & Taxonomy
Protein names
- Recommended nameCarbamoyl phosphate synthase small chain
- EC number
- Alternative names
Gene names
Organism names
- Organism
- Strain
- Taxonomic lineageArchaea > Thermoproteota > Thermoprotei > Sulfolobales > Sulfolobaceae > Acidianus
Accessions
- Primary accessionF4B9S5
Proteomes
Interaction
Subunit
Composed of two chains; the small (or glutamine) chain promotes the hydrolysis of glutamine to ammonia, which is used by the large (or ammonia) chain to synthesize carbamoyl phosphate. Tetramer of heterodimers (alpha,beta)4.
Protein-protein interaction databases
Structure
Family & Domains
Features
Showing features for region, domain.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Region | 1-182 | CPSase | ||||
Sequence: MICKRGYKGYIYLEDGTLIEGCGFGAKGIKAGEVVFTTSMNGYPESLTDPSYRGQILVITHPLVGNYGIPKPIVENGILRNFESERIQVEGLVVAEETDPYKWNSQMSLHDWLKSEGVPGVSDVDTRMLVKKVRNKGVMMGVISSGIELDDPKKYLEKKYDEIDFTQFTSPKSPIIHQGNGK | ||||||
Domain | 7-144 | Carbamoyl-phosphate synthase small subunit N-terminal | ||||
Sequence: YKGYIYLEDGTLIEGCGFGAKGIKAGEVVFTTSMNGYPESLTDPSYRGQILVITHPLVGNYGIPKPIVENGILRNFESERIQVEGLVVAEETDPYKWNSQMSLHDWLKSEGVPGVSDVDTRMLVKKVRNKGVMMGVIS |
Sequence similarities
Belongs to the CarA family.
Belongs to the CarB family.
Keywords
- Domain
Phylogenomic databases
Family and domain databases
Sequence
- Sequence statusComplete
- Length370
- Mass (Da)41,360
- Last updated2011-06-28 v1
- ChecksumA9092DA9BE227015
Sequence databases
Nucleotide Sequence | Protein Sequence | Molecule Type | Status | |
---|---|---|---|---|
CP002535 EMBL· GenBank· DDBJ | AEE93991.1 EMBL· GenBank· DDBJ | Genomic DNA |